Biopython/C2/Manipulating-Sequences/Khasi

Time	Narration
00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Manipulating Sequences.
00:06 Ha kane ka tutorial, ngin pyndonkam ki tools ka Biopython: Ban pynmih ia u random DNA sequence
00:13 Phiah ia u DNA sequence ha ki jaka ba lah buh
00:17 Pyndait lang ia artylli ki sequences ban pynlong kawei ka sequence ba thymmai
00:22 Wad ia ka jingjrong jong ka sequence
00:26 Niew ia ki number jong ki base lane ki dkhot jong u string
00:31 Wad ia u base ba la thmu lane bynta jong u string
00:35 Pynkylla ia ka sequence object sha ka mutable sequence object
00:40 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka undergraduate Biochemistry lane Bioinformatics
00:47 Bad ka basic Python programming
00:51 Lada em, peit ia ki tutorials Python ha ka link ba la ai
00:56 Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 14.10
01:03 Python version 2.7.8
01:07 Ipython interpreter version 2.3.0
01:12 Biopython version 1.64.
01:16 To ngin ia plie iaka terminal bad sdang ia ka ipython interpreter
01:21 Nion lang ia u Ctrl, Alt and t key
01:26 Ha ka prompt, type: "ipython" bad sa nion Enter
01:31 Ka Ipython prompt kan sa mih ha ka screen
01:35 Da kaba pyndonkam ia ka Biopython, ngi lah ban pynmih ia ka sequence object na ka bynta ka random DNA sequence ha kano kano ka jingjrong
01:44 To mynta ngin ia pynmih ia ka sequence object na ka bynta ka DNA sequence kaba 20 bases
01:50 Ha ka prompt, type: "import random", bad nion Enter.
01:56 Nangta, import Seq module na Bio package.
02:01 Bunsien Seq ngi ju pronounce kum seek.
02:06 Ha ka prompt, type: From Bio dot Seq import Seq. Nion Enter.
02:15 Ngin pyndonkam da ka Bio.Alphabet module ban pyntikna ia ki alphabets ha ka DNA sequence
02:22 Type: from Bio dot Alphabet import generic underscore dna. Nion Enter.
02:32 Type ia ki command harum ban shna ia ka sequence object na ka bynta ka randon DNA sequence.
02:38 Buh ia ka sequence ha ka variable dna1
02:42 Sngewbha peit: ha kane ka command, pyndonkam ar tylli ki single quotes ha ka jaka ka double quote. Nion Enter
02:50 Na ka bynta ka output, type: dna1. Nion Enter
02:55 Ka output ka pyni ia ka sequence object na ka bynta ka random DNA sequence.
03:00 Lada phi kwah ka sequence ba thymmai, nion u up-arrow key ban ioh ia ka juh ka command kum haneng. Nion Enter.
03:11 Na ka bynta ka output, type ia ka kyrteng ka variable dna1. Nion Enter
03:17 Ka output ka pyni iaka DNA sequence ba thymmai kaba pher na kaba nyngkong.
03:23 Shaphang ka Sequence Objects
03:25 Ka sequence objects ha ka jingshisha ka long kum ka Python strings.
03:30 Te, bud beit ia ka rukom leh kumba leh ia ka Python strings.
03:35 Ha ka Python, ngi niew ia ki characters ha ka string kaba sdang na 0, ha jaka u 1.
03:41 U character ba nyngkong ha ka sequence u dei u zero.
03:45 Phai biang sha ka terminal.
03:47 Teng teng ngi hap ban trei tang shi bynta jong ka sequence
03:52 Mynta, ngin ia peit kumno ban sei ia ki bynta jong ka string bad buh ia ki kum ki sequence objects.
03:58 Nuksa, ngin slice ia ka DNA sequence ha ki ar bynta.
04:04 Nyngkong, hapdeng ki bases 6 bad 7.
04:08 Kane kan sei ia ka fragment na kaba sdang jong ka sequence sha ka base ba 6 ha ka sequence.
04:15 Ka slice ba ar kan dei hapdeng ka bases 11 bad 12.
04:20 Ka fragment ba ar kan dei na ka base ba 12 shaduh ba kut jong ka sequence.
04:26 Type ia ka command ba harum, ha ka prompt, ban sei ia ka fragment ba nyngkong.
04:31 String1 equal to dna1 within brackets 0 semi-colon 6.
04:39 string1 ka dei ka variable ban lum ia ka fragment ba nyngkong.
04:43 Ki command ba bud kin long beit kum ha ka Python.
04:47 Hapoh kine ki brackets dei ki jaka ba sdang bad ba sangeh ba ba la pyniakhlad da u colon.
04:53 Kine ki jaka ki kynthup lang ia kaba sdang hynrei khlem kynthup ïaki ha ka jaka ba sangeh. Nion Enter.
05:01 Ban peit ia ka output, type: "string1", nion Enter.
05:04 Ka output ka pyni ia ka fragment ba nyngkong kum ka sequence object.
05:10 Ban sei ia ka string ba ar na ka sequence, nion ia u up-arrow key bad edit ia ka command kumne harum:
05:17 Pynkylla ia ka ka kyrteng jong u variable sha ka string2 bad jaka sha u 11 bad 20.
05:24 Na ka bynta ka output, type: "string2". Nion Enter.
05:30 Mynta ngi ioh ia ka fragment ba ar ruh kum ka sequence object.
05:34 To ngin pynlong ïaki kawei, kata, ban pyndait lang ia baroh ar ki strings ban pynlong u fragment ba thymmai.
05:42 Buh ia ka sequence ba thymmai ha ka variable dna2.
05:46 Type: dna2 equal to string1 plus string2. Nion Enter.
05:53 Sngewbha kynmaw ba: ngim lah ban thep ki sequence da ki alphabets ki bym iahap.
05:59 Kata ka mut, ngim lah ban pynlong kawei ia ka DNA sequence bad ka protein sequence ban ioh ia ka sequence ba thymmai.
06:07 Ki ar tylli ki sequences ki dei ban don kajuh ka alphabet attribute.
06:12 Ban peit ia ka output, type: "dna2". Nion Enter.
06:17 Ka output ka pyni ia ka sequence ba thymmai kaba dei ka jing pyndait lang jong u string1 bad string2.
06:23 Ban ioh ia ka jingjrong jong ka sequence ba thymmai, ngin pyndonkam da ka len function.
06:29 Type: "len" hapoh parenthesis"dna2". Nion Enter.
06:34 Ka output ka pyni ia ka sequence kaba jrong 15 bases.
06:39 Ngi lah ruh ban niew ia ka jingdon jong ki bases bapher bapher ha ka sequence.
06:44 Ban leh ia kane, ngin pyndonkam da ka count function.
06:47 Kum ka nuksa – ban niew ia ka jingdon jong u alanines ba don ha ka sequence, ngi type ia ka command ka ba harum: dna2 dot count hapoh parenthesis hapoh double quotes alphabet A.
07:02 Nion Enter.
07:04 Ka output ka pyni ia ka jingdon jong u alanines ha ka sequence dna2.
07:10 Ban shem ia u jait base ne u dkhot jong u string, ngin pyndonkam da u find function.
07:16 Type: dna2 dot find hapoh parenthesis hapoh double quotes "GC". Nion Enter.
07:26 Ka output ka pyni ia ka jaka ba nyngkong kaba mih ka GC ha u string.
07:32 Ha ka jingshisha ia ka sequence object um ju lah ban pynkylla.
07:35 Ban pynkylla ia ka sequence, ngi dei ban pynkylla iaka sha ka mutable sequence object.
07:41 Ban leh ia kane, type: dna3 equal to dna2 dot to mutable plie bad khang ia u parenthesis. Nion Enter.
07:52 Na ka bynta ka output, type: dna3 Nion Enter.
07:55 Mynta ngin sa lah ban pynkylla ia ka sequence object.
07:59 To ngin ia bujli ia u base na ka sequence.
08:01 Kum ka nuksa – ban bujli ia u base uba don ha ka jaka ba 5 sha ka alanine, type: dna3 within brackets 5 equal to within double quotes alphabet A. Nion Enter.
08:19 Na ka bynta ka output, type: dna3. Nion Enter.
08:24 Khmih thuh ia ka output. Ka cytosine ha ka jaka ba 5 la shah bujli da u alanine.
08:31 Ban bujli ia ka shi bynta jong u string, type ia ka command ba harum.
08:35 Dna3 within brackets 6 semi-colon 10 equal to within double quotes ATGC. Nion Enter.
08:45 Na ka bynta ka output, type: dna3. Nion Enter.
08:52 Ka output ka pyni ia ki 4 tylli ki bases na ka jaka ba 6 haduh 9 kiba shah bujli da ki bases ATGC ba thymmai.
09:01 Shisien ba phi lahdep dep pynkylla ia ka sequence object, pynkylla biang ia ka sha ka “read only” form.
09:07 Type kumne harumdna4 equal to dna3 dot to seq open and close parenthesis. Nion Enter.
09:19 Na ka bynta ka output, type: dna4. Nion Enter.
09:25 To ngin batai lyngkot
09:27 Ha kane ka tutorial, ngi lah pule ban * Generate a random DNA sequence
09:32 Slice ia ka DNA sequence ha ki jaka ba lah buh.
09:36 Pyndait lang ar tylli ki sequence ban ioh ia ka sequence ba thymmai, kata ka mut, ban pynlong kawei.
09:43 Ngi lah pule ruh kumno ban: * use len, count bad find functions.
09:49 Ban pynkylla ia ka sequence object sha ka mutable sequence object bad ban bujli ia u base lane shi bynta jong u string.
09:57 Na ka bynta ka assignment, wad ia uno uno u DNA sequence uba 30 bases.
10:02 Da kaba pyndonkam da u Biopython tools, khein ia ka GC percentage bad molecular weight jong ka sequence.
10:09 Ka assignment ba lah dep jong phi kan long kumne harum.
10:13 Ka output ka pyni ia ka GCcontent ha ka percentage.
10:18 Ka output ka pyni ia ka molecular weight jong ka DNA sequence.
10:23 Kane ka video ka batai lyngkot ia ka Spoken Tutorial project.
10:26 Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ia ka.
10:30 Ngi pynlong workshops bad ai certificates.
10:32 Sngewbha thoh sha ngi
10:35 Ia ka Spoken-Tutorial project la kyrshan da ka National Mission on Education through ICT, MHRD, Government of India.
10:43 ïa kane ka script la pynkylla sha ka Ktien Khasi da u Yuwanki Yolande Kharlukhi na Shillong, Bad ma nga u Hezekiah Lyngdoh, ngan pynkut noh. Khublei ba phi la ïasnoh lang.

Contributors and Content Editors

Hezekiah2016, PoojaMoolya