Biopython/C2/Manipulating-Sequences/Khasi
From Script | Spoken-Tutorial
| Time | Narration |
| 00:01 | Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Manipulating Sequences. |
| 00:06 | Ha kane ka tutorial, ngin pyndonkam ki tools ka Biopython: Ban pynmih ia u random DNA sequence |
| 00:13 | Phiah ia u DNA sequence ha ki jaka ba lah buh |
| 00:17 | Pyndait lang ia artylli ki sequences ban pynlong kawei ka sequence ba thymmai |
| 00:22 | Wad ia ka jingjrong jong ka sequence |
| 00:26 | Niew ia ki number jong ki base lane ki dkhot jong u string |
| 00:31 | Wad ia u base ba la thmu lane bynta jong u string |
| 00:35 | Pynkylla ia ka sequence object sha ka mutable sequence object |
| 00:40 | Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka undergraduate Biochemistry lane Bioinformatics |
| 00:47 | Bad ka basic Python programming |
| 00:51 | Lada em, peit ia ki tutorials Python ha ka link ba la ai |
| 00:56 | Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 14.10 |
| 01:03 | Python version 2.7.8 |
| 01:07 | Ipython interpreter version 2.3.0 |
| 01:12 | Biopython version 1.64. |
| 01:16 | To ngin ia plie iaka terminal bad sdang ia ka ipython interpreter |
| 01:21 | Nion lang ia u Ctrl, Alt and t key |
| 01:26 | Ha ka prompt, type: "ipython" bad sa nion Enter |
| 01:31 | Ka Ipython prompt kan sa mih ha ka screen |
| 01:35 | Da kaba pyndonkam ia ka Biopython, ngi lah ban pynmih ia ka sequence object na ka bynta ka random DNA sequence ha kano kano ka jingjrong |
| 01:44 | To mynta ngin ia pynmih ia ka sequence object na ka bynta ka DNA sequence kaba 20 bases |
| 01:50 | Ha ka prompt, type: "import random", bad nion Enter. |
| 01:56 | Nangta, import Seq module na Bio package. |
| 02:01 | Bunsien Seq ngi ju pronounce kum seek. |
| 02:06 | Ha ka prompt, type: From Bio dot Seq import Seq. Nion Enter. |
| 02:15 | Ngin pyndonkam da ka Bio.Alphabet module ban pyntikna ia ki alphabets ha ka DNA sequence |
| 02:22 | Type: from Bio dot Alphabet import generic underscore dna. Nion Enter. |
| 02:32 | Type ia ki command harum ban shna ia ka sequence object na ka bynta ka randon DNA sequence. |
| 02:38 | Buh ia ka sequence ha ka variable dna1 |
| 02:42 | Sngewbha peit: ha kane ka command, pyndonkam ar tylli ki single quotes ha ka jaka ka double quote. Nion Enter |
| 02:50 | Na ka bynta ka output, type: dna1. Nion Enter |
| 02:55 | Ka output ka pyni ia ka sequence object na ka bynta ka random DNA sequence. |
| 03:00 | Lada phi kwah ka sequence ba thymmai, nion u up-arrow key ban ioh ia ka juh ka command kum haneng. Nion Enter. |
| 03:11 | Na ka bynta ka output, type ia ka kyrteng ka variable dna1. Nion Enter |
| 03:17 | Ka output ka pyni iaka DNA sequence ba thymmai kaba pher na kaba nyngkong. |
| 03:23 | Shaphang ka Sequence Objects |
| 03:25 | Ka sequence objects ha ka jingshisha ka long kum ka Python strings. |
| 03:30 | Te, bud beit ia ka rukom leh kumba leh ia ka Python strings. |
| 03:35 | Ha ka Python, ngi niew ia ki characters ha ka string kaba sdang na 0, ha jaka u 1. |
| 03:41 | U character ba nyngkong ha ka sequence u dei u zero. |
| 03:45 | Phai biang sha ka terminal. |
| 03:47 | Teng teng ngi hap ban trei tang shi bynta jong ka sequence |
| 03:52 | Mynta, ngin ia peit kumno ban sei ia ki bynta jong ka string bad buh ia ki kum ki sequence objects. |
| 03:58 | Nuksa, ngin slice ia ka DNA sequence ha ki ar bynta. |
| 04:04 | Nyngkong, hapdeng ki bases 6 bad 7. |
| 04:08 | Kane kan sei ia ka fragment na kaba sdang jong ka sequence sha ka base ba 6 ha ka sequence. |
| 04:15 | Ka slice ba ar kan dei hapdeng ka bases 11 bad 12. |
| 04:20 | Ka fragment ba ar kan dei na ka base ba 12 shaduh ba kut jong ka sequence. |
| 04:26 | Type ia ka command ba harum, ha ka prompt, ban sei ia ka fragment ba nyngkong. |
| 04:31 | String1 equal to dna1 within brackets 0 semi-colon 6. |
| 04:39 | string1 ka dei ka variable ban lum ia ka fragment ba nyngkong. |
| 04:43 | Ki command ba bud kin long beit kum ha ka Python. |
| 04:47 | Hapoh kine ki brackets dei ki jaka ba sdang bad ba sangeh ba ba la pyniakhlad da u colon. |
| 04:53 | Kine ki jaka ki kynthup lang ia kaba sdang hynrei khlem kynthup ïaki ha ka jaka ba sangeh. Nion Enter. |
| 05:01 | Ban peit ia ka output, type: "string1", nion Enter. |
| 05:04 | Ka output ka pyni ia ka fragment ba nyngkong kum ka sequence object. |
| 05:10 | Ban sei ia ka string ba ar na ka sequence, nion ia u up-arrow key bad edit ia ka command kumne harum: |
| 05:17 | Pynkylla ia ka ka kyrteng jong u variable sha ka string2 bad jaka sha u 11 bad 20. |
| 05:24 | Na ka bynta ka output, type: "string2". Nion Enter. |
| 05:30 | Mynta ngi ioh ia ka fragment ba ar ruh kum ka sequence object. |
| 05:34 | To ngin pynlong ïaki kawei, kata, ban pyndait lang ia baroh ar ki strings ban pynlong u fragment ba thymmai. |
| 05:42 | Buh ia ka sequence ba thymmai ha ka variable dna2. |
| 05:46 | Type: dna2 equal to string1 plus string2. Nion Enter. |
| 05:53 | Sngewbha kynmaw ba: ngim lah ban thep ki sequence da ki alphabets ki bym iahap. |
| 05:59 | Kata ka mut, ngim lah ban pynlong kawei ia ka DNA sequence bad ka protein sequence ban ioh ia ka sequence ba thymmai. |
| 06:07 | Ki ar tylli ki sequences ki dei ban don kajuh ka alphabet attribute. |
| 06:12 | Ban peit ia ka output, type: "dna2". Nion Enter. |
| 06:17 | Ka output ka pyni ia ka sequence ba thymmai kaba dei ka jing pyndait lang jong u string1 bad string2. |
| 06:23 | Ban ioh ia ka jingjrong jong ka sequence ba thymmai, ngin pyndonkam da ka len function. |
| 06:29 | Type: "len" hapoh parenthesis"dna2". Nion Enter. |
| 06:34 | Ka output ka pyni ia ka sequence kaba jrong 15 bases. |
| 06:39 | Ngi lah ruh ban niew ia ka jingdon jong ki bases bapher bapher ha ka sequence. |
| 06:44 | Ban leh ia kane, ngin pyndonkam da ka count function. |
| 06:47 | Kum ka nuksa – ban niew ia ka jingdon jong u alanines ba don ha ka sequence, ngi type ia ka command ka ba harum: dna2 dot count hapoh parenthesis hapoh double quotes alphabet A. |
| 07:02 | Nion Enter. |
| 07:04 | Ka output ka pyni ia ka jingdon jong u alanines ha ka sequence dna2. |
| 07:10 | Ban shem ia u jait base ne u dkhot jong u string, ngin pyndonkam da u find function. |
| 07:16 | Type: dna2 dot find hapoh parenthesis hapoh double quotes "GC". Nion Enter. |
| 07:26 | Ka output ka pyni ia ka jaka ba nyngkong kaba mih ka GC ha u string. |
| 07:32 | Ha ka jingshisha ia ka sequence object um ju lah ban pynkylla. |
| 07:35 | Ban pynkylla ia ka sequence, ngi dei ban pynkylla iaka sha ka mutable sequence object. |
| 07:41 | Ban leh ia kane, type: dna3 equal to dna2 dot to mutable plie bad khang ia u parenthesis. Nion Enter. |
| 07:52 | Na ka bynta ka output, type: dna3 Nion Enter. |
| 07:55 | Mynta ngin sa lah ban pynkylla ia ka sequence object. |
| 07:59 | To ngin ia bujli ia u base na ka sequence. |
| 08:01 | Kum ka nuksa – ban bujli ia u base uba don ha ka jaka ba 5 sha ka alanine, type: dna3 within brackets 5 equal to within double quotes alphabet A. Nion Enter. |
| 08:19 | Na ka bynta ka output, type: dna3. Nion Enter. |
| 08:24 | Khmih thuh ia ka output. Ka cytosine ha ka jaka ba 5 la shah bujli da u alanine. |
| 08:31 | Ban bujli ia ka shi bynta jong u string, type ia ka command ba harum. |
| 08:35 | Dna3 within brackets 6 colon 10 equal to within double quotes ATGC. Nion Enter. |
| 08:45 | Na ka bynta ka output, type: dna3. Nion Enter. |
| 08:52 | Ka output ka pyni ia ki 4 tylli ki bases na ka jaka ba 6 haduh 9 kiba shah bujli da ki bases ATGC ba thymmai. |
| 09:01 | Shisien ba phi lahdep dep pynkylla ia ka sequence object, pynkylla biang ia ka sha ka “read only” form. |
| 09:07 | Type kumne harumdna4 equal to dna3 dot to seq open and close parenthesis. Nion Enter. |
| 09:19 | Na ka bynta ka output, type: dna4. Nion Enter. |
| 09:25 | To ngin batai lyngkot |
| 09:27 | Ha kane ka tutorial, ngi lah pule ban * Generate a random DNA sequence |
| 09:32 | Slice ia ka DNA sequence ha ki jaka ba lah buh. |
| 09:36 | Pyndait lang ar tylli ki sequence ban ioh ia ka sequence ba thymmai, kata ka mut, ban pynlong kawei. |
| 09:43 | Ngi lah pule ruh kumno ban: * use len, count bad find functions. |
| 09:49 | Ban pynkylla ia ka sequence object sha ka mutable sequence object bad ban bujli ia u base lane shi bynta jong u string. |
| 09:57 | Na ka bynta ka assignment, wad ia uno uno u DNA sequence uba 30 bases. |
| 10:02 | Da kaba pyndonkam da u Biopython tools, khein ia ka GC percentage bad molecular weight jong ka sequence. |
| 10:09 | Ka assignment ba lah dep jong phi kan long kumne harum. |
| 10:13 | Ka output ka pyni ia ka GCcontent ha ka percentage. |
| 10:18 | Ka output ka pyni ia ka molecular weight jong ka DNA sequence. |
| 10:23 | Kane ka video ka batai lyngkot ia ka Spoken Tutorial project. |
| 10:26 | Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ia ka. |
| 10:30 | Ngi pynlong workshops bad ai certificates. |
| 10:32 | Sngewbha thoh sha ngi |
| 10:35 | Ia ka Spoken-Tutorial project la kyrshan da ka National Mission on Education through ICT, MHRD, Government of India. |
| 10:43 | ïa kane ka script la pynkylla sha ka Ktien Khasi da u Yuwanki Yolande Kharlukhi na Shillong, Bad ma nga u Hezekiah Lyngdoh, ngan pynkut noh. Khublei ba phi la ïasnoh lang. |
