Jmol-Application/C4/3D-Models-of-Enzymes/Khasi

Time	Narration
00:01	Khublei ïaphi baroh. Pdiang sngewbha sha kane ka tutorial halor 3D Models of Enzymes in Jmol .
00:08	Ha kane ka tutorial, ngin pule ban: * Load structure jong Human Pancreatic Hexokinase haka Jmol panel
00:16	Pynpher ia ka display jong ka secondary structure
00:20	Highlight ki amino acid residue haka active site.
00:25	Highlight substrate bad ki cofactor jong ki enzyme.
00:30	Bad, peit ka Ramachandran plot na bynta u protein.
00:35	Ban bud ïa kane ka tutorial, phi dei ban don ka jing tip ïaka basic biochemistry
00:41	bad tip bha shaphang ka rukom treikam naka Jmol Application window.
00:46	Sngewbha peit ïaka tutorial Proteins and Macromolecules haka Jmol Application series.
00:53	lah ban ioh ïaka na ka link harum.
00:57	Ban record ïa kane ka tutorial, nga pyndonkam ka:* Ubuntu Operating System version 12.04
01:05	Jmol version 12.2.2.
01:08	Java version 7 bad * Mozilla Firefox browser 22.0.
01:16	Plie ïaka Jmol window bad load ka structure jong u hexokinase enzyme.
01:22	Nga long connect sha ka internet. Te, ngan load beit ïaka structure beit beit naka PDB website.
01:28	Ban leh kumta, plie ïaka File menu, hiar shapoh bad click ha u jingjied Get PDB.
01:37	Ka Input dialogue-box ka paw haka screen. Type ïaki saw tylli ki dak PDB code na bynta ka hexokinase, kata, 3IDH haka text-box.
01:50	ïa une u code la ioh na ka website jong ka Protein Data Bank.
01:55	Lada phim don ka internet connection ba long: plie ïaka file pdb pyndonkam da u icon Open a file ha u tool bar.
02:06	Click ha u jingshon OK.
02:09	ïa ki 3D structure jong u hexokinase bala tip ruh kum ka glucokinase la plie haka screen.
02:16	Plie ïaka Console window pyndonkam daka File menu.
02:21	Kumba la pyni ha ka Console, ka structure haka panel ka dei naka bynta ka Human Pancreatic Glucokinase ryngkat ka substrate Glucose.
02:31	Khang noh ka Console.
02:34	Haka panel, ngi don ïaka model ball and stick jong ka hexokinase.
02:40	Weng ïaki water molecules na ka protein model haka panel.
02:44	ïa Kane ka rukom la batai bniah bha haka Jmol tutorial Proteins and macromolecules.
02:53	Shaphang u hexokinase Enzyme-
02:56	Hexokinase u dei u monomeric protein jong ki 465 amino acids.
03:02	u don artylli ki domains, Ka domain baheh bad ka domain barit.
03:07	Ka active-site na bynta kane ka enzyme la pynshong haka cleft hapdeng ki artylli ki domain.
03:14	Ka Active-site na bynta ka hexokinase ka don 3 tylli ki amino acid residue: Aspergine ha 204, Aspergine haka kyrdan ba 231 bad Glutamic acid ha 256.
03:30	U Alpha-D-Glucose u dei u substrate naka bynta une u enzyme.
03:34	Mynta to ngin leit phai pat sha ka Jmol panel.
03:38	Ngi lah ban jied bad highlight ïa ki jingpynlong jong u enzymes kum Substrate, Cofactors lane Amino acid residues haka active site.
03:49	Ban jied ïa uno uno u component- plie ïaka pop-up menu dakaba pyndonkam u right-click.
03:55	Hiar sha u jingjied Select.
03:57	Naka sub-menu, Proteins, jied By Residue name.
04:04	Ngi don ki amino acid residue ba marwei bala list hangne.
04:10	Click haka kyrteng jong u amino acid ban jied ïa u.
04:14	ïa ki amino acids ruh la group hapoh ki heading kum: Polar, Non-polar, Basic, Acidic, Uncharged ter ter.
04:26	Kiba la List haka Hetero menu, ki long ki metal ion potassium bad substrate glucose.
04:34	Ngi lah ban pynpher ïaka display jong u enzyme ban lap suk ïa ka substrate binding site.`
04:41	To ngin pynkylla ïaka display bad ka rong jong ki atoms jong ki protein.
04:46	Plie ïaka pop-up menu, leit sha Select bad hiar sha u jingjied Protein. Click ha All.
04:55	Plie biang ïaka pop-up menu, Hiar sha u Style, nangta sha u Scheme. bAd click ha u jingjied Sticks.
05:05	Mynta ngi don haka panel, ïa ka protein haka sticks display.
05:11	Mynta ban pynkylla ïa ka rong, - plie biang ïaka pop-up menu, leit sha Color >> Atoms bad click ha u jingjied Blue.
05:23	Ngi don haka screen ïaka model jong ka hexokinase haka rong blue bad haka sticks display.
05:30	Khmih thuh ïa u substrate Alfa-D-Glucose haka ball and stick display haka cleft.
05:38	Ban highlight ïa ka substrate- plie ïaka pop-up menu, leit sha Select, nangta Hetero bad click ha GLC-ALFA-D-GLUCOSE.
05:52	Plie biang ïaka pop-up menu, Hiar sha Style >> Scheme bad click ha u jingjied Sticks.
06:00	Ban pynkylla ïaka rong – Plie biang ïaka pop-up menu, Hiar sha Color >> Atoms bad click ha u jingjied White.
06:12	Haka panel, ïa u model jong ka hexokinase ryngkat ka jaka shong jong u substrate la highlight shai bha.
06:20	Ngi lah ban pynkylla ïa ka rong jong ki amino acids haka active site ban highlight ïaki.
06:26	ban leh kumta, ngi donkam ban type ki command ha ka Console window.
06:32	Kumba la la batai mynne, ki amino acids kiba don haka active-site ki long u Aspergine ha ka kyrdan ba 204, Aspergine haka kyrdan ba 231 bad Glutamic acid ha 256.
06:50	Plie ïaka console window pyndonkam ka File menu. Click ha Console.
06:57	Nga pyndonkam ka Kmag Screen magnifier ban pyni heh ïaka console window.
07:03	Ha u $ (dollar) prompt, type: "select" hapoh ki square brackets "Asn" na bynta u aspergine bad khang noh u bracket, "204" kata, ka kyrdan semicolon "color atoms orange".
07:25	ñion Enter.
07:27	Khmih thuh ba ki atom jong u aspargine residue mynta ki long rong orange.
07:33	ñion ïa u up-arrow button haka key board bad edit ïaka command.
07:39	Edit ïaka jaka shong u amino acid sha 231 bad ka rong jong ki atom sha rong saw.
07:48	ñion Enter.
07:51	ñion biang ïa u up-arrow key bad edit ïaka kyrteng jong u amino acid sha GLU, kata u, glutamic acid bad ka kyrdan sha 256.
08:06	bad, ka rong jong ki atom sha rong green bad ñion Enter.
08:13	Ngi don haka panel ïa ka 3D model jong ka hexokinase ryngkat ka substrate bad ka active site bala highlight.
08:23	Bala highlight ruh haka model u dei u potassium atom bala pyni hangne,haka rong purple.
08:30	Ngi lah ruh ban pyni ïa ki ramachandran plots na bynta u protein bala thmu haka jmol.
08:36	Ha ka console, ha u dollar($) prompt, type: plot ramachandran
08:45	ñion Enter.
08:47	Ha ka screen, ngi don ka ramachandran plot na ka bynta ka hexokinase.
08:54	Pyrshang ban load ïa ki enzymes bapher bapher pyndonkam da ki pdb files naka database.
09:00	Pynkylla ïaka display jong ka secondary structure.
09:04	To ngin Batai lyngkot. Ha kane ka tutorial, ngi la pule ban:: * Load ki structure jong ka Human Pancreatic Hexokinase pyndonkam u PDB code.
09:14	Pynpher ïaka display jong ka secondary structure.
09:17	Highlight ki amino acid residue haka active site.
09:21	Highlight ki substrate bad ki cofactor jong ki enzyme.
09:25	Bad,Peit ïa ka ramachandran plot naka bynta ki proteins.
09:30	Kum ka assignment: Load ïa ka dot pdb file jong u enzyme Lysozyme haka Jmol panel.
09:38	Highlight ïa u substrate bala khum sha u enzyme.
09:42	Highlight ki amino acids haka active site.
09:46	Hint: Shim ïa ka pdb file jong Lysozyme naka PDB database.
09:52	Peit ïaka video badon ha kane ka URL:
09:56	Ka batai lyngkot ïaka Spoken Tutorial project.
10:00	Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ïaka.
10:04	Ka Spoken Tutorial project team:
10:07	Ka pynlong workshop bad Ka sam certificates.
10:10	Ban tip kham bniah, sngewbha thoh sha ngi
10:14	Spoken Tutorial project kadei shi bynta jong ka ‘‘‘Talk to a Teacher project.
10:19	La kyrshan ïaka daka National Mission on Education Lyngba ka ICT, MHRD, Sorkar India.
10:25	Khambun ka jingtip halor kane ka mission kadon ha kane ka link:
10:30	ïa kane ka script la pynkylla sha ka ktien Khasi da I Hezekiah Lyngdoh na Shillong. Khublei ba phi la ïasnohlang.

Contributors and Content Editors

Hezekiah2016, PoojaMoolya