Jmol-Application/C4/3D-Models-of-Enzymes/Khasi
From Script | Spoken-Tutorial
| Time | Narration |
| 00:01 | Khublei ïaphi baroh. Pdiang sngewbha sha kane ka tutorial halor 3D Models of Enzymes in Jmol . |
| 00:08 | Ha kane ka tutorial, ngin pule ban: * Load structure jong Human Pancreatic Hexokinase haka Jmol panel |
| 00:16 | Pynpher ia ka display jong ka secondary structure |
| 00:20 | Highlight ki amino acid residue haka active site. |
| 00:25 | Highlight substrate bad ki cofactor jong ki enzyme. |
| 00:30 | Bad, peit ka Ramachandran plot na bynta u protein. |
| 00:35 | Ban bud ïa kane ka tutorial, phi dei ban don ka jing tip ïaka basic biochemistry |
| 00:41 | bad tip bha shaphang ka rukom treikam naka Jmol Application window. |
| 00:46 | Sngewbha peit ïaka tutorial Proteins and Macromolecules haka Jmol Application series. |
| 00:53 | lah ban ioh ïaka na ka link harum. |
| 00:57 | Ban record ïa kane ka tutorial, nga pyndonkam ka:* Ubuntu Operating System version 12.04 |
| 01:05 | Jmol version 12.2.2. |
| 01:08 | Java version 7 bad * Mozilla Firefox browser 22.0. |
| 01:16 | Plie ïaka Jmol window bad load ka structure jong u hexokinase enzyme. |
| 01:22 | Nga long connect sha ka internet. Te, ngan load beit ïaka structure beit beit naka PDB website. |
| 01:28 | Ban leh kumta, plie ïaka File menu, hiar shapoh bad click ha u jingjied Get PDB. |
| 01:37 | Ka Input dialog-box ka paw haka screen. Type ïaki saw tylli ki dak PDB code na bynta ka hexokinase, kata, 3IDH haka text-box. |
| 01:50 | ïa une u code la ioh na ka website jong ka Protein Data Bank. |
| 01:55 | Lada phim don ka internet connection ba long: plie ïaka file pdb pyndonkam da u icon Open a file ha u tool bar. |
| 02:06 | Click ha u jingshon OK. |
| 02:09 | ïa ki 3D structure jong u hexokinase bala tip ruh kum ka glucokinase la plie haka screen. |
| 02:16 | Plie ïaka Console window pyndonkam daka File menu. |
| 02:21 | Kumba la pyni ha ka Console, ka structure haka panel ka dei naka bynta ka Human Pancreatic Glucokinase ryngkat ka substrate Glucose. |
| 02:31 | Khang noh ka Console. |
| 02:34 | Haka panel, ngi don ïaka model ball and stick jong ka hexokinase. |
| 02:40 | Weng ïaki water molecules na ka protein model haka panel. |
| 02:44 | ïa Kane ka rukom la batai bniah bha haka Jmol tutorial Proteins and macromolecules. |
| 02:53 | Shaphang u hexokinase Enzyme- |
| 02:56 | Hexokinase u dei u monomeric protein jong ki 465 amino acids. |
| 03:02 | u don artylli ki domains, Ka domain baheh bad ka domain barit. |
| 03:07 | Ka active-site na bynta kane ka enzyme la pynshong haka cleft hapdeng ki artylli ki domain. |
| 03:14 | Ka Active-site na bynta ka hexokinase ka don 3 tylli ki amino acid residue: Aspergine ha 204, Aspergine haka kyrdan ba 231 bad Glutamic acid ha 256. |
| 03:30 | U Alpha-D-Glucose u dei u substrate naka bynta une u enzyme. |
| 03:34 | Mynta to ngin leit phai pat sha ka Jmol panel. |
| 03:38 | Ngi lah ban jied bad highlight ïa ki jingpynlong jong u enzymes kum Substrate, Cofactors lane Amino acid residues haka active site. |
| 03:49 | Ban jied ïa uno uno u component- plie ïaka pop-up menu dakaba pyndonkam u right-click. |
| 03:55 | Hiar sha u jingjied Select. |
| 03:57 | Naka sub-menu, Proteins, jied By Residue name. |
| 04:04 | Ngi don ki amino acid residue ba marwei bala list hangne. |
| 04:10 | Click haka kyrteng jong u amino acid ban jied ïa u. |
| 04:14 | ïa ki amino acids ruh la group hapoh ki heading kum: Polar, Non-polar, Basic, Acidic, Uncharged ter ter. |
| 04:26 | Kiba la List haka Hetero menu, ki long ki metal ion potassium bad substrate glucose. |
| 04:34 | Ngi lah ban pynpher ïaka display jong u enzyme ban lap suk ïa ka substrate binding site.` |
| 04:41 | To ngin pynkylla ïaka display bad ka rong jong ki atoms jong ki protein. |
| 04:46 | Plie ïaka pop-up menu, leit sha Select bad hiar sha u jingjied Protein. Click ha All. |
| 04:55 | Plie biang ïaka pop-up menu, Hiar sha u Style, nangta sha u Scheme. bAd click ha u jingjied Sticks. |
| 05:05 | Mynta ngi don haka panel, ïa ka protein haka sticks display. |
| 05:11 | Mynta ban pynkylla ïa ka rong, - plie biang ïaka pop-up menu, leit sha Color >> Atoms bad click ha u jingjied Blue. |
| 05:23 | Ngi don haka screen ïaka model jong ka hexokinase haka rong blue bad haka sticks display. |
| 05:30 | Khmih thuh ïa u substrate Alfa-D-Glucose haka ball and stick display haka cleft. |
| 05:38 | Ban highlight ïa ka substrate- plie ïaka pop-up menu, leit sha Select, nangta Hetero bad click ha GLC-ALFA-D-GLUCOSE. |
| 05:52 | Plie biang ïaka pop-up menu, Hiar sha Style >> Scheme bad click ha u jingjied Sticks. |
| 06:00 | Ban pynkylla ïaka rong – Plie biang ïaka pop-up menu, Hiar sha Color >> Atoms bad click ha u jingjied White. |
| 06:12 | Haka panel, ïa u model jong ka hexokinase ryngkat ka jaka shong jong u substrate la highlight shai bha. |
| 06:20 | Ngi lah ban pynkylla ïa ka rong jong ki amino acids haka active site ban highlight ïaki. |
| 06:26 | ban leh kumta, ngi donkam ban type ki command ha ka Console window. |
| 06:32 | Kumba la la batai mynne, ki amino acids kiba don haka active-site ki long u Aspergine ha ka kyrdan ba 204, Aspergine haka kyrdan ba 231 bad Glutamic acid ha 256. |
| 06:50 | Plie ïaka console window pyndonkam ka File menu. Click ha Console. |
| 06:57 | Nga pyndonkam ka Kmag Screen magnifier ban pyni heh ïaka console window. |
| 07:03 | Ha u $ (dollar) prompt, type: "select" hapoh ki square brackets "Asn" na bynta u aspergine bad khang noh u bracket, "204" kata, ka kyrdan semicolon "color atoms orange". |
| 07:25 | ñion Enter. |
| 07:27 | Khmih thuh ba ki atom jong u aspargine residue mynta ki long rong orange. |
| 07:33 | ñion ïa u up-arrow button haka key board bad edit ïaka command. |
| 07:39 | Edit ïaka jaka shong u amino acid sha 231 bad ka rong jong ki atom sha rong saw. |
| 07:48 | ñion Enter. |
| 07:51 | ñion biang ïa u up-arrow key bad edit ïaka kyrteng jong u amino acid sha GLU, kata u, glutamic acid bad ka kyrdan sha 256. |
| 08:06 | bad, ka rong jong ki atom sha rong green bad ñion Enter. |
| 08:13 | Ngi don haka panel ïa ka 3D model jong ka hexokinase ryngkat ka substrate bad ka active site bala highlight. |
| 08:23 | Bala highlight ruh haka model u dei u potassium atom bala pyni hangne,haka rong purple. |
| 08:30 | Ngi lah ruh ban pyni ïa ki ramachandran plots na bynta u protein bala thmu haka jmol. |
| 08:36 | Ha ka console, ha u dollar($) prompt, type: plot ramachandran |
| 08:45 | ñion Enter. |
| 08:47 | Ha ka screen, ngi don ka ramachandran plot na ka bynta ka hexokinase. |
| 08:54 | Pyrshang ban load ïa ki enzymes bapher bapher pyndonkam da ki pdb files naka database. |
| 09:00 | Pynkylla ïaka display jong ka secondary structure. |
| 09:04 | To ngin Batai lyngkot. Ha kane ka tutorial, ngi la pule ban:: * Load ki structure jong ka Human Pancreatic Hexokinase pyndonkam u PDB code. |
| 09:14 | Pynpher ïaka display jong ka secondary structure. |
| 09:17 | Highlight ki amino acid residue haka active site. |
| 09:21 | Highlight ki substrate bad ki cofactor jong ki enzyme. |
| 09:25 | Bad,Peit ïa ka ramachandran plot naka bynta ki proteins. |
| 09:30 | Kum ka assignment: Load ïa ka dot pdb file jong u enzyme Lysozyme haka Jmol panel. |
| 09:38 | Highlight ïa u substrate bala khum sha u enzyme. |
| 09:42 | Highlight ki amino acids haka active site. |
| 09:46 | Hint: Shim ïa ka pdb file jong Lysozyme naka PDB database. |
| 09:52 | Peit ïaka video badon ha kane ka URL: |
| 09:56 | Ka batai lyngkot ïaka Spoken Tutorial project. |
| 10:00 | Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ïaka. |
| 10:04 | Ka Spoken Tutorial project team: |
| 10:07 | Ka pynlong workshop bad Ka sam certificates. |
| 10:10 | Ban tip kham bniah, sngewbha thoh sha ngi |
| 10:14 | Spoken Tutorial project kadei shi bynta jong ka ‘‘‘Talk to a Teacher project. |
| 10:19 | La kyrshan ïaka daka National Mission on Education Lyngba ka ICT, MHRD, Sorkar India. |
| 10:25 | Khambun ka jingtip halor kane ka mission kadon ha kane ka link: |
| 10:30 | ïa kane ka script la pynkylla sha ka ktien Khasi da I Hezekiah Lyngdoh na Shillong. Khublei ba phi la ïasnohlang. |
