Jmol-Application/C4/Proteins-and-Macromolecules/Khasi
From Script | Spoken-Tutorial
| Time | Narration |
| 00:01 | Pdiang sngewbha sha kane ka tutorial halor Proteins and Macromolecules. |
| 00:06 | Ha kane ka tutorial, ngin pule ban: |
| 00:09 | Load ki structure jong ki protein na ka Protein Data Bank (PDB) |
| 00:13 | Download ki file '.pdb' naka PDB database |
| 00:18 | Display ka secondary structure jong ki protein haki format ba pher ba pher. |
| 00:24 | Highlight ki hydrogen bonds bad ki disulfide bonds. |
| 00:29 | Ban bud ïa kane ka tutorial, phi dei ban tip bha shaphang ka rukom treikam naka Jmol Application window. |
| 00:37 | Lada em, peit ïa ki tutorial ba kum kane kiba don haka website jong ngi. |
| 00:42 | Ban record ïa kane ka tutorial, nga pyndonkam ka: |
| 00:46 | Ubuntu OS version 12.04 |
| 00:50 | Jmol version 12.2.2 |
| 00:54 | Java version 7 |
| 00:57 | Mozilla Firefox Browser 22.0. |
| 01:02 | Structure Analysis jong ki biomolecules baheh kum ki - |
| 01:06 | Proteins bad Macromolecules |
| 01:10 | Nucleic acids, DNA bad RNA |
| 01:13 | Crystal structures bad polymers lah ban leh dakaba pyndonkam ka Jmol Application. |
| 01:19 | Hangne, Nga la plie ka Jmol window bathymmai. |
| 01:24 | ïaki 3D structure jong ki biomolecules lah ban peit dakaba download beit beit naka database. |
| 01:29 | Ban leh kumta, click ha ka File menu, hiar sha Get PDB. |
| 01:36 | KA Input dialog-box ka mih haka screen. |
| 01:40 | Ngi dei ban type ia ki saw tylli ki dak PDB code naka bynta u protein, bala thmu haka Input box. |
| 01:48 | ïa Une u code lah ban ioh naka Protein Data Bank (PDB) website. |
| 01:53 | Kane ka dei ka web-page jong ka Protein Data Bank. |
| 01:57 | Ka don ïaka thup jingtip shaphang ki biomolecules kum ki proteins bad ki nucleic acids. |
| 02:04 | Kum ka nuksa, To ngin pyrshang ban wad ïa u PDB code naka bynta u Pancreatic Enzyme Insulin naka PDB website. |
| 02:13 | Ha u search box, type ïaka kyrteng jong u protein kum "human insulin". Ñion u Enter key haka keyboard. |
| 02:24 | Mynta, haka web-page kaba la pyni, Hiar shapoh. |
| 02:28 | KA list jong ki Human Insulin structure ba ngi tip ryngkat bad ki PDB codes ka paw haka screen. |
| 02:36 | Kumka nuksa, to ngin jied Human Insulin ryngkat u code 4EX1. |
| 02:44 | Click ha ka kyrteng jong u protein. |
| 02:47 | KA windowka plie ryngkat bad baroh ki jingbatai jong ka structure. |
| 02:52 | Ki thup jingtip kum- |
| 02:54 | Primary Citation |
| 02:56 | Molecular Description bad |
| 02:58 | Structure Validation ki don ha kane ka page. |
| 03:02 | Ngi lah ban save ïa ki structure jong ki proteins kum ki file '.pdb' bad peit ïa ki haka 3D mode haka Jmol. |
| 03:12 | Click ha ka link Download Files, ba shong ha khlieh ka mon ha kyndong jong ka page. |
| 03:20 | Naka drop-down menu, jied ïa u jingjied PDB file (gz) . |
| 03:28 | KA dialog-box ka plie haka screen. |
| 03:32 | JIed ïa u jingjied Save file. |
| 03:35 | Click ha u jingshon OK. |
| 03:39 | Ka structure jong u protein ynsa save kum 4EX1.pdb.gz, haka Downloads folder. |
| 03:51 | Kumjuh ruh, phi lah ban download ïaki file '.pdb' ba donkam jong ki proteins bapher bapher bad save ïaki hala ki jong ki jong ki file. |
| 04:02 | Mynta, to ngin leit pat sha ka Jmol window ban peit ïa ka 3D structure jong u Insulin. |
| 04:09 | Lada phi long connect sha ka internet, phi lah ban download beit beit ïa ka structure u protein haka Jmol panel. |
| 04:15 | Type ïaki saw tylli ki dak PDB code “4EX1” haka text-box bad click ha u jingshon OK. |
| 04:25 | Lada phim long connect sha ka internet te click ha u icon Open a File ha u tool-bar. |
| 04:34 | KA dialog-box ka plie ha ka panel. |
| 04:38 | ïaid sha ka jaka shong jong ka file 4EX1.pdb.gz . kata. Sha ka Downloads folder. |
| 04:47 | JIed ïaka Downloads folder bad click ha u jingshon Open . |
| 04:52 | JIed ïaka file 4EX1.pdb.gz bad click ha u jingshon Open. |
| 05:00 | Ka '3D' Structure jong u Insulin ka plie haka screen. |
| 05:05 | Ka display balong hi jong u protein haka panel ka dei ball and stick. |
| 05:12 | ïaka model jong u protein haka panel la pyni khlem ki hydrogen atoms. |
| 05:17 | Ban pyni ïa u model ryngkat ki hydrogen atoms, plie ïaka modelkit menu. |
| 05:23 | Hiar sha u jingjied add hydrogens bad click ha u. |
| 05:28 | Mynta la pyni ïa U model ryngkat ki hydrogen atom haka panel. |
| 05:33 | ka protein structure la pyni ruh ryngkat ki water molecules. |
| 05:38 | Ban buhrieh ïaki water molecules, bud ïaki synjam harum kumba la pyni. |
| 05:43 | Nyngkong, plie ïaka pop-up menu bad leit sha Select. |
| 05:48 | Naka sub-menu, Jied Hetero bad click ha u jingjied “All Water. |
| 05:55 | Plie biang ïaka pop-up menu, leit sha Style >> Scheme bad click ha u jingjied CPK spacefill . |
| 06:05 | Kane kan pynkylla ïa baroh ki water molecules sha ka CPK Spacefill display. |
| 06:11 | Plie biang ïaka pop-up menu bad leit sha Style, hiar sha Atoms bad click ha u jingjied Off . |
| 06:22 | Mynta ngi don ka Insulin structure bakhlem kano kano ka water molecules ha ka panel. |
| 06:27 | Nangta, to ngin pule kumno ban pyni ïa ka structure ba –ar jong u protein haki format bapher bapher. |
| 06:35 | Plie ïaka pop-up menu. |
| 06:37 | Leit sha u jingjied Select . |
| 06:39 | Hiar sha u Protein bad click ha u jingjied All . |
| 06:44 | Plie biang ïa ka pop-up menu bad hiar sha Style, nangta Scheme. |
| 06:50 | Ka sub-menu ka plie bad ki jingjied kum CPK Spacefill, Ball and Stick, Sticks, Wireframe, cartoon, trace ter ter. |
| 07:02 | Click ha u jingjied Cartoon naka sub-menu. |
| 07:07 | Kane ka display ka pyni ïa ka structure ba- ar jong u protein kum helices, random coils, strands, sheets ter ter. |
| 07:17 | Na bynta ki jingjied ba khambun jong ka - |
| 07:19 | Plie ïa ka pop-up menu bad hiar sha Style, nangta sha Structures. |
| 07:25 | Hangne , ngi iohi shuh shuh ki jingjied ban pyni ïaki structure ba-ar jong u protein. |
| 07:31 | Kum ka nuksa, click ha u jingjied Strands. |
| 07:35 | ïa u protein la pyni mynta haka panel kum ki Strands. |
| 07:40 | Ban pynkylla ïaka rong jong ka display: Plie ïaka Pop-up-menu. Hiar sha Color. Jied Atoms bad click ha u jingjied Blue. |
| 07:52 | Khmih ïaka jingkylla rong ha ka panel. |
| 07:56 | Ban pynkylla biang ïaka structure sha ka Ball-and-stick display: |
| 07:59 | Plie ïaka pop-up menu, jied Style, nangta Scheme bad click ha u jingjied Ball and stick . |
| 08:08 | Ngi lah ruh ban highlight ki hydrogen bonds bad di-sulpfide bonds ha u protein model. |
| 08:14 | Ban display ki hydrogen bonds: Plie ïaka pop-up menu bad Hiar sha u Style bad nangta sha u jingjied Hydrogen Bonds. |
| 08:25 | Ki jingjied jong The Hydrogen Bonds haka pop-up menu ka don ki jinglong kiba harum: |
| 08:30 | Calculate, Set Hydrogen Bonds Side Chain, |
| 08:35 | Set Hydrogen Bonds in the Backbone bad ruh ka don ki jingjied ban pynkylla ïaka jingrben jong ki bond. |
| 08:42 | Click ha u jingjied Calculate ban pyni ïaki hydrogen bonds haka model. |
| 08:47 | ïa ki hydrogen bonds la pyni kum ki dash bajrong haka rong lieh bad rong saw. |
| 08:53 | Ban pynkylla ïaka jingrben jong ki hydrogen bonds, click ha u jingjied 0.10 A naka pop-up-menu. |
| 09:02 | Mynta ngi lah ban iohi ïaka jingrben jong ki hydrogen bonds haka panel. |
| 09:06 | Ngi lah ruh ban pynkylla ïaka rong jong ki hydrogen bonds. |
| 09:11 | Naka Pop-up-menu, Hiar sha Color, nangta Hydrogen Bonds, nangta click ha u jingjied orange. |
| 09:20 | Ngi don ïa baroh ki hydrogen bonds rong orange haka panel. |
| 09:25 | Ki Disulfide bonds bad sulphur atoms la pyni haka model, haki rong yellow. |
| 09:31 | Ban pynpher ïaki disulfide bonds, plie u jingjied disulfide bonds haka pop-up menu. |
| 09:38 | Click ha ki jing long kiba phi kwah ban pynkylla, kum ki, size, color ter ter. |
| 09:44 | Kumjuh ruh, pyrshang ban plie ïaki file '.pdb' jong ki enzymes bapher bad peit ïaki 3D structure jong ki. |
| 09:51 | To ngin Batai lyngkot. Ha kane ka tutorial, ngi la pule ban: |
| 09:57 | Load ki structure jong ki protein naka Protein Data Bank (PDB). |
| 10:00 | Download ki file '.pdb' naka database. |
| 10:05 | Peit ïaka 3D structure jong u insulin pyndonkam u PDB code. |
| 10:10 | Peit ka Protein structure ba khlem ki water molecules. |
| 10:14 | Display ka secondary structure haki format bapher bapher. |
| 10:17 | Highlight ki hydrogen bonds bad disulfide bonds. |
| 10:22 | Kane ka assignment naka bynta jong phi- |
| 10:24 | Download ïaka file '.pdb' jong ka Human Hemoglobin naka PDB database. |
| 10:31 | Pyni ia ka secondary structure haka cartoon display. |
| 10:35 | Highlight ki “porphyrin” units jong u protein. |
| 10:39 | Peit naka ling harum naka bynta u PDB code. |
| 10:42 | Peit ïaka video badon ha kane ka URL: |
| 10:46 | Ka batai lyngkot ïaka Spoken Tutorial project. |
| 10:50 | Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ïaka. |
| 10:55 | Ka Spoken Tutorial Project team: |
| 10:57 | Ka pynlong workshop dakaba pyndonkam ki spoken tutorials. |
| 11:01 | Ka ai certificates sha kito kiba pass ïaka on-line test. |
| 11:06 | Ban tip kham bniah, sngewbha thoh sha:
contact@spoken-tutorial.org |
| 11:13 | Spoken Tutorial project kadei shi bynta jong ka ‘‘‘Talk to a Teacher project. |
| 11:18 | La kyrshan ïaka daka National Mission on Education Lyngba ka ICT, MHRD, Sorkar India.
|
| 11:25 | Khambun ka jingtip halor kane ka mission kadon ha kane ka link harum: |
| 11:31 | ïa kane ka script la pynkylla sha ka ktien Khasi da I Hezekiah Lyngdoh na Shillong. Khublei ba phi la ïasnohlang. |
