Biopython/C2/Introduction-to-Biopython/Khasi

Time	Narration
00:01	Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Introduction to Biopython.
00:05	Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka Biopython
00:10	Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System
00:15	Bad ka translation ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka Biopython
00:22	Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka
00:25	Undergraduate Biochemistry lane Bioinformatics
00:29	bad ka basic Python programming
00:31	Peit ia ki Python' tutorial ha ka link bala ai
00:35	Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 12.04
00:41	Python version 2.7.3
00:44	Ipython version 0.12.1 bad
00:48	Biopython version 1.58.
00:51	Ka Biopython ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology
00:57	ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics
01:03	Ia ki tiar jong ka Biopython lah ju pyndonkam na ka bynta
01:05	Parsing, kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka FASTA, Genbank, bad kumta ter ter
01:14	Ban download data na ka database websites kum ka NCBI, ExPASY, bad kumta ter ter
01:22	Ban Run ïaki Bioinformatic algorithms kum ka BLAST.
01:26	Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih
01:31	Kum ka nuksa – ban ioh complements, transcription, translation bad kumta ter ter
01:38	Ki code ban peit ia ka alignment
01:40	bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher
01:46	Jingpyntip shaphang ban download
01:48	Ka Biopython kam dei kaba wan lang bad ka Python; ngi hap ban download ïaka laka jong
01:54	Ban tip kham bniah, peit ia ka link ba harum.
01:59	Ban install ha ka system Linux
02:02	Install ia ka Python, Ipython bad Biopythonda kaba pyndonkam da ka Synaptic Package Manager
02:08	Ki software ba donkam kin sa install hi
02:13	Ngi hap ban install hi ia ki software jong ka graphic outputs bad plots
02:18	Plie ia ka terminal da kaba nion lang ia u Ctrl, Alt bad T key
02:24	Nga lah dep install lypa ia ka Python, Ipython bad Biopython ha system jong nga
02:30	Plie ia ka Ipython interpreter da kaba type "ipython" bad sa nion Enter
02:35	Ka IPythonprompt ka paw ha screen
02:38	Ban pyntikna ba lah install ia kaBiopython, ha ka prompt, type "import Bio", bad sa press Enter
02:48	Lada phim ioh message error ei ei, ka mut ka Biopython lah install
02:54	Hangne, to ngan pynkynmaw ia phi ba kaPython language ka long case sensitive
02:59	Da phikir bha haba type ki keywords, ki variables lane functions
03:04	Kumba long, u lain haneng, u, “i” ha import dei u lowercase bad u “B”dei u uppercase ha Bio
03:12	Ha kane ka tutorial, ngin pyndonkam da ka Biopythonmodules ban pynkylla ia ka DNA sequence
03:19	Ki rukom pyndonkam ki long harum
03:22	Nyngkong eh, shna ia ka sequence object ban code ia ka DNA strand
03:27	Nangta, sa ka transcription ban code ia ka DNA strand sha ka mRNA
03:32	Khatduh eh, translation jong ka mRNA sha ka protein sequence.
03:37	Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa
03:42	Ka code naka bynta u protein sequence barit
03:46	ka synjam ba nyngkong ka dei ban shna ia ka sequence object na ka bynta ka coding DNA strand kaba hajrong
03:52	To ngin ia phai biang sha ka terminal
03:55	Ban shna ia ka sequence object, import ïaka ka Seq module na ka Bio package
04:02	Ka Seq module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects
04:08	Ha ka prompt, type: from Bio dot Seq import Seq bad sa nion Enter
04:17	Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka sequence object jong phi.
04:24	Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka nucleotides lane amino acids
04:32	Ban leh kumta, ngin pyndonkam da ka IUPAC module na ka Alphabet package
04:38	Ha ka prompt, type: from Bio dot Alphabet import IUPAC. Sa nion Enter
04:48	Kynmaw ba ngi lah pyndonkam import bad from statements ban load "Seq" bad "IUPAC" modules
04:56	Buh ia u sequence object ha ka variable kaba kyrteng cdna
05:01	Ha ka prompt, type: cdna equal to Seq kum ha ki normal string
05:08	Pyndait ia ki sequence hapoh ki double quotes bad parentheses
05:13	Ngi tip ba ka sequence jong ngi ka dei ka DNA ba lyngkhot. Te, type unambiguous DNA alphabet object kum ka argument
05:21	Na ka bynta ka output, type: cdna. Nion Enter
05:26	Ka output ka pyni ia ka DNA sequence kum ka sequence object
05:30	To ngin thoh ia ka coding DNA strand sha ka mRNA. Ba ïadei bad kane.
05:35	Ngin pyndonkam da ka rukom Seq module's built-in “transcribe”
05:39	Type ia ki code kiba harum
05:41	Buh ia ka output ha ka variable mrna
05:45	Ha ka prompt, type: mrna equal to cdna dot transcribe open and close parentheses, nion Enter
05:55	Na ka bynta ka output, type: mrna. Nion Enter
06:01	Khmih thuh ia ka output. Ka rukom transcribe kan bujli ia ka Thiamin ha ka DNA sequence da ka Uracil
06:09	Kaba bud, ban translate ia kane ka mRNA sha ka corresponding protein sequence pyndonkam da ka rukom jong ka translate
06:16	Type ia kine ki code harum: protein equal to mrna dot translate open and close parentheses. Nion Enter
06:27	Ka translate method RNA lane DNA sequence da kaba pyndom ia ka standard genetic code
06:36	Ka output ka pyni ia ka amino acid sequence.
06:40	Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka stop codons ha ka sequence lah pynkylla.
06:47	Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka stop codon.
06:53	Ha u code ba ha jrong, ngi lah pyndonkam ia u coding DNA strand na ka bynta ka transcription
06:59	Ha Biopython, ka transcribemethod ka treikam tang ha ka coding DNA strand
07:04	Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka transcription ka sdang da ka template strand
07:11	Lada phi sdang da ka template strand, pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka reverse complement method, kumba lah pyni ha ka terminal.
07:20	Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand
07:24	Da kaba pyndonkam da ki rukom ha Biopython ngi la dep ban pynkylla ia ka DNA sequence sha ka protein sequence.
07:31	Ka DNA sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code
07:37	To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang:
07:41	Ki jinglong ba kongsan jong ka Biopython
07:43	Ka thup jingtip shaphang ka download bad installation ha Linux OS
07:48	Ban shna sequence object na ka bynta ka DNA strand
07:52	Transcription ia ka DNA sequence sha ka mRNA
07:56	Translation ia ka mRNA sha ka protein sequence.
08:00	Mynta, na ka bynta ka assignment
08:02	Pynkylla ka DNA sequence ba lah ai sha ka protein sequence.
08:06	Khmih thuh ia ka output
08:08	Ka protein sequence ka don ia ka stop codon
08:11	Kumba ka ju long shisha, pynkylla ia ka DNA shaduh U stop codonuba nyngkong haka frame
08:17	Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum
08:20	Peit thuh ba ngi lah pyndonkam ka argumentto underscore stop ha ka translate method. Peit ka output
08:27	Ia u stop codon hi khlem shym la pynkylla
08:31	U stop symbol u khlem shah kynthup ha kaba kut ka protein sequence.
08:36	Kane ka video ka batai lyngkot ia ka Spoken Tutorial project
08:39	Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka
08:43	Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test
08:50	Ban tip kham bniah, sngewbha thoh sha ngi
08:53	Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India
08:59	Khambuh ki jingtip halor kane ka mission ka don ha kane ka link
09:03	ïa kane ka script la pynkylla sha ka Ktien Khasi da i Yuwanki Yolande Kharlukhi na Shillong, khublei ba phi la ïasnoh lang.

Contributors and Content Editors

Hezekiah2016