Biopython/C2/Introduction-to-Biopython/Khasi
From Script | Spoken-Tutorial
Revision as of 15:36, 23 April 2018 by Hezekiah2016 (Talk | contribs)
| Time | Narration | ||
| 00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Introduction to Biopython.
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| 00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka Biopython | |||
| 00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System | |||
| 00:15 Bad ka translation ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka Biopython | |||
| 00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka | |||
| 00:25 Undergraduate Biochemistry lane Bioinformatics | |||
| 00:29 bad ka basic Python programming | |||
| 00:31 Peit ia ki Python' tutorial ha ka link bala ai | |||
| 00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 12.04 | |||
| 00:41 Python version 2.7.3 | |||
| 00:44 Ipython version 0.12.1 bad | |||
| 00:48 Biopython version 1.58. | |||
| 00:51 Ka Biopython ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology | |||
| 00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics | |||
| 01:03 Ia ki tiar jong ka Biopython lah ju pyndonkam na ka bynta | |||
| 01:05 Parsing, kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka FASTA, Genbank, bad kumta ter ter
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| 01:14 Ban download data na ka database websites kum ka NCBI, ExPASY, bad kumta ter ter | |||
| 01:22 | Ban Run ïaki Bioinformatic algorithms kum ka BLAST. | ||
| 01:26 | Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih | ||
| 01:31 | Kum ka nuksa – ban ioh complements, transcription, translation bad kumta ter ter | ||
| 01:38 | Ki code ban peit ia ka alignment | ||
| 01:40 | bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher | ||
| 01:46 | Jingpyntip shaphang ban download | ||
| 01:48 Ka Biopython kam dei kaba wan lang bad ka Python; ngi hap ban download ïaka laka jong | |||
| 01:54 | Ban tip kham bniah, peit ia ka link ba harum. | ||
| 01:59 | Ban install ha ka system Linux | ||
| 02:02 | Install ia ka Python, Ipython bad Biopythonda kaba pyndonkam da ka Synaptic Package Manager | ||
| 02:08 | Ki software ba donkam kin sa install hi | ||
| 02:13 | Ngi hap ban install hi ia ki software jong ka graphic outputs bad plots | ||
| 02:18 | Plie ia ka terminal da kaba nion lang ia u Ctrl, Alt bad T key | ||
| 02:24 | Nga lah dep install lypa ia ka Python, Ipython bad Biopython ha system jong nga | ||
| 02:30 | Plie ia ka Ipython interpreter da kaba type "ipython" bad sa nion Enter | ||
| 02:35 | Ka IPythonprompt ka paw ha screen | ||
| 02:38 | Ban pyntikna ba lah install ia kaBiopython, ha ka prompt, type "import Bio", bad sa press Enter | ||
| 02:48 | Lada phim ioh message error ei ei, ka mut ka Biopython lah install | ||
| 02:54 | Hangne, to ngan pynkynmaw ia phi ba kaPython language ka long case sensitive | ||
| 02:59 | Da phikir bha haba type ki keywords, ki variables lane functions | ||
| 03:04 | Kumba long, u lain haneng, u, “i” ha import dei u lowercase bad u “B”dei u uppercase ha Bio | ||
| 03:12 | Ha kane ka tutorial, ngin pyndonkam da ka Biopythonmodules ban pynkylla ia ka DNA sequence | ||
| 03:19 | Ki rukom pyndonkam ki long harum | ||
| 03:22 | Nyngkong eh, shna ia ka sequence object ban code ia ka DNA strand | ||
| 03:27 | Nangta, sa ka transcription ban code ia ka DNA strand sha ka mRNA | ||
| 03:32 | Khatduh eh, translation jong ka mRNA sha ka protein sequence. | ||
| 03:37 | Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa | ||
| 03:42 | Ka code naka bynta u protein sequence barit | ||
| 03:46 | ka synjam ba nyngkong ka dei ban shna ia ka sequence object na ka bynta ka coding DNA strand kaba hajrong | ||
| 03:52 | To ngin ia phai biang sha ka terminal | ||
| 03:55 | Ban shna ia ka sequence object, import ïaka ka Seq module na ka Bio package | ||
| 04:02 | Ka Seq module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects | ||
| 04:08 | Ha ka prompt, type: from Bio dot Seq import Seq bad sa nion Enter | ||
| 04:17 | Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka sequence object jong phi. | ||
| 04:24 | Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka nucleotides lane amino acids | ||
| 04:32 | Ban leh kumta, ngin pyndonkam da ka IUPAC module na ka Alphabet package | ||
| 04:38 | Ha ka prompt, type: from Bio dot Alphabet import IUPAC. Sa nion Enter | ||
| 04:48 | Kynmaw ba ngi lah pyndonkam import bad from statements ban load "Seq" bad "IUPAC" modules | ||
| 04:56 | Buh ia u sequence object ha ka variable kaba kyrteng cdna | ||
| 05:01 | Ha ka prompt, type: cdna equal to Seq kum ha ki normal string | ||
| 05:08 | Pyndait ia ki sequence hapoh ki double quotes bad parentheses | ||
| 05:13 | Ngi tip ba ka sequence jong ngi ka dei ka DNA ba lyngkhot. Te, type unambiguous DNA alphabet object kum ka argument | ||
| 05:21 | Na ka bynta ka output, type: cdna. Nion Enter | ||
| 05:26 | Ka output ka pyni ia ka DNA sequence kum ka sequence object | ||
| 05:30 | To ngin thoh ia ka coding DNA strand sha ka mRNA. Ba ïadei bad kane. | ||
| 05:35 | Ngin pyndonkam da ka rukom Seq module's built-in “transcribe” | ||
| 05:39 | Type ia ki code kiba harum | ||
| 05:41 | Buh ia ka output ha ka variable mrna | ||
| 05:45 | Ha ka prompt, type: mrna equal to cdna dot transcribe open and close parentheses, nion Enter | ||
| 05:55 | Na ka bynta ka output, type: mrna. Nion Enter
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| 06:01 | Khmih thuh ia ka output. Ka rukom transcribe kan bujli ia ka Thiamin ha ka DNA sequence da ka Uracil | ||
| 06:09 | Kaba bud, ban translate ia kane ka mRNA sha ka corresponding protein sequence pyndonkam da ka rukom jong ka translate | ||
| 06:16 | Type ia kine ki code harum: protein equal to mrna dot translate open and close parentheses. Nion Enter | ||
| 06:27 | Ka translate method RNA lane DNA sequence da kaba pyndom ia ka standard genetic code | ||
| 06:36 | Ka output ka pyni ia ka amino acid sequence. | ||
| 06:40 | Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka stop codons ha ka sequence lah pynkylla. | ||
| 06:47 | Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka stop codon. | ||
| 06:53 | Ha u code ba ha jrong, ngi lah pyndonkam ia u coding DNA strand na ka bynta ka transcription | ||
| 06:59 | Ha Biopython, ka transcribemethod ka treikam tang ha ka coding DNA strand | ||
| 07:04 | Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka transcription ka sdang da ka template strand | ||
| 07:11 | Lada phi sdang da ka template strand, pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka reverse complement method, kumba lah pyni ha ka terminal. | ||
| 07:20 | Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand | ||
| 07:24 | Da kaba pyndonkam da ki rukom ha Biopython ngi la dep ban pynkylla ia ka DNA sequence sha ka protein sequence. | ||
| 07:31 | Ka DNA sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code | ||
| 07:37 | To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang: | ||
| 07:41 | Ki jinglong ba kongsan jong ka Biopython | ||
| 07:43 | Ka thup jingtip shaphang ka download bad installation ha Linux OS | ||
| 07:48 | Ban shna sequence object na ka bynta ka DNA strand | ||
| 07:52 | Transcription ia ka DNA sequence sha ka mRNA | ||
| 07:56 | Translation ia ka mRNA sha ka protein sequence. | ||
| 08:00 | Mynta, na ka bynta ka assignment | ||
| 08:02 | Pynkylla ka DNA sequence ba lah ai sha ka protein sequence. | ||
| 08:06 | Khmih thuh ia ka output | ||
| 08:08 | Ka protein sequence ka don ia ka stop codon | ||
| 08:11 | Kumba ka ju long shisha, pynkylla ia ka DNA shaduh U stop codonuba nyngkong haka frame | ||
| 08:17 | Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum | ||
| 08:20 | Peit thuh ba ngi lah pyndonkam ka argumentto underscore stop ha ka translate method. Peit ka output | ||
| 08:27 | Ia u stop codon hi khlem shym la pynkylla | ||
| 08:31 | U stop symbol u khlem shah kynthup ha kaba kut ka protein sequence. | 08:36 | Kane ka video ka batai lyngkot ia ka Spoken Tutorial project |
| 08:39 | Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka | ||
| 08:43 | Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test | ||
| 08:50 | Ban tip kham bniah, sngewbha thoh sha ngi | ||
| 08:53 | Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India | ||
| 08:59 | Khambuh ki jingtip halor kane ka mission ka don ha kane ka link | ||
| 09:03 | } |
