Jmol-Application/C4/Proteins-and-Macromolecules/Khasi

Time	Narration
00:01	Pdiang sngewbha sha kane ka tutorial halor Proteins and Macromolecules.
00:06	Ha kane ka tutorial, ngin pule ban:
00:09	Load ki structure jong ki protein na ka Protein Data Bank (PDB)
00:13	Download ki file '.pdb' naka PDB database
00:18	Display ka secondary structure jong ki protein haki format ba pher ba pher.
00:24	Highlight ki hydrogen bonds bad ki disulfide bonds.
00:29	Ban bud ïa kane ka tutorial, phi dei ban tip bha shaphang ka rukom treikam naka Jmol Application window.
00:37	Lada em, peit ïa ki tutorial ba kum kane kiba don haka website jong ngi.
00:42	Ban record ïa kane ka tutorial, nga pyndonkam ka:
00:46	Ubuntu OS version 12.04
00:50	Jmol version 12.2.2
00:54	Java version 7
00:57	Mozilla Firefox Browser 22.0.
01:02	Structure Analysis jong ki biomolecules baheh kum ki -
01:06	Proteins bad Macromolecules
01:10	Nucleic acids, DNA bad RNA
01:13	Crystal structures bad polymers lah ban leh dakaba pyndonkam ka Jmol Application.
01:19	Hangne, Nga la plie ka Jmol window bathymmai.
01:24	ïaki 3D structure jong ki biomolecules lah ban peit dakaba download beit beit naka database.
01:29	Ban leh kumta, click ha ka File menu, hiar sha Get PDB.
01:36	KA Input dialog-box ka mih haka screen.
01:40	Ngi dei ban type ia ki saw tylli ki dak PDB code naka bynta u protein, bala thmu haka Input box.
01:48	ïa Une u code lah ban ioh naka Protein Data Bank (PDB) website.
01:53	Kane ka dei ka web-page jong ka Protein Data Bank.
01:57	Ka don ïaka thup jingtip shaphang ki biomolecules kum ki proteins bad ki nucleic acids.
02:04	Kum ka nuksa, To ngin pyrshang ban wad ïa u PDB code naka bynta u Pancreatic Enzyme Insulin naka PDB website.
02:13	Ha u search box, type ïaka kyrteng jong u protein kum "human insulin". Ñion u Enter key haka keyboard.
02:24	Mynta, haka web-page kaba la pyni, Hiar shapoh.
02:28	KA list jong ki Human Insulin structure ba ngi tip ryngkat bad ki PDB codes ka paw haka screen.
02:36	Kumka nuksa, to ngin jied Human Insulin ryngkat u code 4EX1.
02:44	Click ha ka kyrteng jong u protein.
02:47	KA windowka plie ryngkat bad baroh ki jingbatai jong ka structure.
02:52	Ki thup jingtip kum-
02:54	Primary Citation
02:56	Molecular Description bad
02:58	Structure Validation ki don ha kane ka page.
03:02	Ngi lah ban save ïa ki structure jong ki proteins kum ki file '.pdb' bad peit ïa ki haka 3D mode haka Jmol.
03:12	Click ha ka link Download Files, ba shong ha khlieh ka mon ha kyndong jong ka page.
03:20	Naka drop-down menu, jied ïa u jingjied PDB file (gz) .
03:28	KA dialog-box ka plie haka screen.
03:32	JIed ïa u jingjied Save file.
03:35	Click ha u jingshon OK.
03:39	Ka structure jong u protein ynsa save kum 4EX1.pdb.gz, haka Downloads folder.
03:51	Kumjuh ruh, phi lah ban download ïaki file '.pdb' ba donkam jong ki proteins bapher bapher bad save ïaki hala ki jong ki jong ki file.
04:02	Mynta, to ngin leit pat sha ka Jmol window ban peit ïa ka 3D structure jong u Insulin.
04:09	Lada phi long connect sha ka internet, phi lah ban download beit beit ïa ka structure u protein haka Jmol panel.
04:15	Type ïaki saw tylli ki dak PDB code “4EX1” haka text-box bad click ha u jingshon OK.
04:25	Lada phim long connect sha ka internet te click ha u icon Open a File ha u tool-bar.
04:34	KA dialog-box ka plie ha ka panel.
04:38	ïaid sha ka jaka shong jong ka file 4EX1.pdb.gz . kata. Sha ka Downloads folder.
04:47	JIed ïaka Downloads folder bad click ha u jingshon Open .
04:52	JIed ïaka file 4EX1.pdb.gz bad click ha u jingshon Open.
05:00	Ka '3D' Structure jong u Insulin ka plie haka screen.
05:05	Ka display balong hi jong u protein haka panel ka dei ball and stick.
05:12	ïaka model jong u protein haka panel la pyni khlem ki hydrogen atoms.
05:17	Ban pyni ïa u model ryngkat ki hydrogen atoms, plie ïaka modelkit menu.
05:23	Hiar sha u jingjied add hydrogens bad click ha u.
05:28	Mynta la pyni ïa U model ryngkat ki hydrogen atom haka panel.
05:33	ka protein structure la pyni ruh ryngkat ki water molecules.
05:38	Ban buhrieh ïaki water molecules, bud ïaki synjam harum kumba la pyni.
05:43	Nyngkong, plie ïaka pop-up menu bad leit sha Select.
05:48	Naka sub-menu, Jied Hetero bad click ha u jingjied “All Water.
05:55	Plie biang ïaka pop-up menu, leit sha Style >> Scheme bad click ha u jingjied CPK spacefill .
06:05	Kane kan pynkylla ïa baroh ki water molecules sha ka CPK Spacefill display.
06:11	Plie biang ïaka pop-up menu bad leit sha Style, hiar sha Atoms bad click ha u jingjied Off .
06:22	Mynta ngi don ka Insulin structure bakhlem kano kano ka water molecules ha ka panel.
06:27	Nangta, to ngin pule kumno ban pyni ïa ka structure ba –ar jong u protein haki format bapher bapher.
06:35	Plie ïaka pop-up menu.
06:37	Leit sha u jingjied Select .
06:39	Hiar sha u Protein bad click ha u jingjied All .
06:44	Plie biang ïa ka pop-up menu bad hiar sha Style, nangta Scheme.
06:50	Ka sub-menu ka plie bad ki jingjied kum CPK Spacefill, Ball and Stick, Sticks, Wireframe, cartoon, trace ter ter.
07:02	Click ha u jingjied Cartoon naka sub-menu.
07:07	Kane ka display ka pyni ïa ka structure ba- ar jong u protein kum helices, random coils, strands, sheets ter ter.
07:17	Na bynta ki jingjied ba khambun jong ka -
07:19	Plie ïa ka pop-up menu bad hiar sha Style, nangta sha Structures.
07:25	Hangne , ngi iohi shuh shuh ki jingjied ban pyni ïaki structure ba-ar jong u protein.
07:31	Kum ka nuksa, click ha u jingjied Strands.
07:35	ïa u protein la pyni mynta haka panel kum ki Strands.
07:40	Ban pynkylla ïaka rong jong ka display: Plie ïaka Pop-up-menu. Hiar sha Color. Jied Atoms bad click ha u jingjied Blue.
07:52	Khmih ïaka jingkylla rong ha ka panel.
07:56	Ban pynkylla biang ïaka structure sha ka Ball-and-stick display:
07:59	Plie ïaka pop-up menu, jied Style, nangta Scheme bad click ha u jingjied Ball and stick .
08:08	Ngi lah ruh ban highlight ki hydrogen bonds bad di-sulpfide bonds ha u protein model.
08:14	Ban display ki hydrogen bonds: Plie ïaka pop-up menu bad Hiar sha u Style bad nangta sha u jingjied Hydrogen Bonds.
08:25	Ki jingjied jong The Hydrogen Bonds haka pop-up menu ka don ki jinglong kiba harum:
08:30	Calculate, Set Hydrogen Bonds Side Chain,
08:35	Set Hydrogen Bonds in the Backbone bad ruh ka don ki jingjied ban pynkylla ïaka jingrben jong ki bond.
08:42	Click ha u jingjied Calculate ban pyni ïaki hydrogen bonds haka model.
08:47	ïa ki hydrogen bonds la pyni kum ki dash bajrong haka rong lieh bad rong saw.
08:53	Ban pynkylla ïaka jingrben jong ki hydrogen bonds, click ha u jingjied 0.10 A naka pop-up-menu.
09:02	Mynta ngi lah ban iohi ïaka jingrben jong ki hydrogen bonds haka panel.
09:06	Ngi lah ruh ban pynkylla ïaka rong jong ki hydrogen bonds.
09:11	Naka Pop-up-menu, Hiar sha Color, nangta Hydrogen Bonds, nangta click ha u jingjied orange.
09:20	Ngi don ïa baroh ki hydrogen bonds rong orange haka panel.
09:25	Ki Disulfide bonds bad sulphur atoms la pyni haka model, haki rong yellow.
09:31	Ban pynpher ïaki disulfide bonds, plie u jingjied disulfide bonds haka pop-up menu.
09:38	Click ha ki jing long kiba phi kwah ban pynkylla, kum ki, size, color ter ter.
09:44	Kumjuh ruh, pyrshang ban plie ïaki file '.pdb' jong ki enzymes bapher bad peit ïaki 3D structure jong ki.
09:51	To ngin Batai lyngkot. Ha kane ka tutorial, ngi la pule ban:
09:57	Load ki structure jong ki protein naka Protein Data Bank (PDB).
10:00	Download ki file '.pdb' naka database.
10:05	Peit ïaka 3D structure jong u insulin pyndonkam u PDB code.
10:10	Peit ka Protein structure ba khlem ki water molecules.
10:14	Display ka secondary structure haki format bapher bapher.
10:17	Highlight ki hydrogen bonds bad disulfide bonds.
10:22	Kane ka assignment naka bynta jong phi-
10:24	Download ïaka file '.pdb' jong ka Human Hemoglobin naka PDB database.
10:31	Pyni ia ka secondary structure haka cartoon display.
10:35	Highlight ki “porphyrin” units jong u protein.
10:39	Peit naka ling harum naka bynta u PDB code.
10:42	Peit ïaka video badon ha kane ka URL: http://spoken-tutorial.org/What_is_a_Spoken_Tutorial
10:46	Ka batai lyngkot ïaka Spoken Tutorial project.
10:50	Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ïaka.
10:55	Ka Spoken Tutorial Project team:
10:57	Ka pynlong workshop dakaba pyndonkam ki spoken tutorials.
11:01	Ka ai certificates sha kito kiba pass ïaka on-line test.
11:06	Ban tip kham bniah, sngewbha thoh sha: contact@spoken-tutorial.org
11:13	Spoken Tutorial project kadei shi bynta jong ka ‘‘‘Talk to a Teacher project.
11:18	La kyrshan ïaka daka National Mission on Education Lyngba ka ICT, MHRD, Sorkar India.
11:25	Khambun ka jingtip halor kane ka mission kadon ha kane ka link harum: http://spoken-tutorial.org/NMEICT-Intro.
11:31	ïa kane ka script la pynkylla sha ka ktien Khasi da I Hezekiah Lyngdoh na Shillong. Khublei ba phi la ïasnohlang.

Contributors and Content Editors

Hezekiah2016