Difference between revisions of "UCSF-Chimera/C3/Superimposing-and-Morphing/English-timed"
From Script | Spoken-Tutorial
Sandhya.np14 (Talk | contribs) |
Sandhya.np14 (Talk | contribs) |
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− | | This structure is bound to the | + | | This structure is bound to the '''substrate analog Farnesyl thiopyrophosphate''', '''FPS''' in short. |
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| 01:46 | | 01:46 | ||
− | |For this we will fetch a structure of the same enzyme without substrate. | + | |For this, we will fetch a structure of the same enzyme without substrate. |
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| 01:52 | | 01:52 | ||
− | | On the '''command line''' type “'''open 2zco'''” press | + | | On the '''command line''', type: “'''open 2zco'''”, press '''Enter'''. |
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− | | Now these two structures are ready to be | + | | Now, these two structures are ready to be superimposed. |
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| 02:10 | | 02:10 | ||
− | | '''Superimposition or Structural alignment''' is a tool to compare two or more '''protein''' structures. | + | | '''Superimposition''' or '''Structural alignment''' is a tool to compare two or more '''protein''' structures. |
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| 02:18 | | 02:18 | ||
− | |The alignment is based on their shape and three-dimensional conformation. | + | |The alignment is based on their shape and three-dimensional '''conformation'''. |
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| 02:24 | | 02:24 | ||
− | | Before we start | + | | Before we start superimposing, let us place some '''icon'''s in the tool bar. |
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| 02:30 | | 02:30 | ||
− | | Click on '''Favorites | + | | Click on '''Favorites''' menu. |
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| 02:32 | | 02:32 | ||
− | | | + | |Scroll down to '''Add to favorites/Tool bar '''option. |
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| 02:53 | | 02:53 | ||
− | | Check boxes for | + | | Check boxes for '''Command line''' , '''Model Panel''' , '''Side View'''. |
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| 02:59 | | 02:59 | ||
− | | Scroll down and click on | + | | Scroll down and click on '''MatchMaker ''' and '''Match-Align'''. |
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| 03:08 | | 03:08 | ||
− | |Select | + | |Select '''tool'''s according to your requirement. |
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| 03:12 | | 03:12 | ||
− | | Observe the panel. | + | | Observe the '''panel'''. |
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| 03:43 | | 03:43 | ||
− | | Now let us '''superimpose''' the structures using '''MatchMaker''' function. | + | | Now, let us '''superimpose''' the structures using '''MatchMaker''' function. |
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|04:01 | |04:01 | ||
− | |For now, let us continue with the default | + | |For now, let us continue with the '''default setting'''s. Press '''OK''' button. |
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| 04:09 | | 04:09 | ||
− | |The two structures are | + | |The two structures are superimposed on each other. |
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|04:13 | |04:13 | ||
− | | The two structures are almost completely | + | | The two structures are almost completely superimposable with each other |
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|04:19 | |04:19 | ||
− | | | + | |except for a small part which is non-superimposable. |
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|04:23 | |04:23 | ||
− | | The fragment involved in this | + | | The fragment involved in this non-superimposable part starts from the '''amino acid''' number 53 to '''amino acid''' number 57. |
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|04:55 | |04:55 | ||
− | | To select the | + | | To select the '''chain'''s, click on their '''pdb Ids'''. |
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|05:05 | |05:05 | ||
− | | In this box the '''amino acid '''pairs used in the final fit, are shown with light orange boxes. | + | | In this box, the '''amino acid '''pairs used in the final fit, are shown with light orange boxes. |
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Revision as of 12:43, 28 June 2018
Time | Narration |
00:01 | Welcome to this tutorial on Superimposing and Morphing. |
00:06 | In this tutorial, we will learn to: superimpose and compare different structures of the same protein, |
00:13 | morph conformations and create a trajectory. |
00:17 | To follow this tutorial, you should be familiar with Chimera interface. |
00:22 | If not, for relevant tutorials, please visit our website. |
00:27 | Here I am using :Ubuntu OS version 14.04, Chimera version 1.10.2 , Mozilla firefox browser 42.0 and a working internet connection. |
00:43 | Here, I have opened a Chimera window. |
00:46 | From the graphic access interface, click on the file 3w7f. |
00:53 | The structure of Squalin Synthase opens on the screen. |
00:57 | Now, let us prepare this structure for superimposing. |
01:01 | It contains two copies of the same protein. |
01:05 | Using the command line, delete one of the chains. |
01:09 | I will recall the command by clicking on the command history. |
01:14 | From the command history dialog box, select the command “delete:.a”. |
01:20 | Press Enter. |
01:22 | Next, to remove solvent molecules from the structure, I will select the command “del solvent”. Press Enter. |
01:32 | This structure is bound to the substrate analog Farnesyl thiopyrophosphate, FPS in short. |
01:40 | We will superimpose two similar proteins to compare their secondary structures. |
01:46 | For this, we will fetch a structure of the same enzyme without substrate. |
01:52 | On the command line, type: “open 2zco”, press Enter. |
02:02 | The new structure is in blue color. |
02:05 | Now, these two structures are ready to be superimposed. |
02:10 | Superimposition or Structural alignment is a tool to compare two or more protein structures. |
02:18 | The alignment is based on their shape and three-dimensional conformation. |
02:24 | Before we start superimposing, let us place some icons in the tool bar. |
02:30 | Click on Favorites menu. |
02:32 | Scroll down to Add to favorites/Tool bar option. |
02:37 | A Preferences dialog box opens. |
02:40 | From the Category drop-down, select Tools. |
02:44 | Under the heading “Settings”, check boxes under “On Tool bar” column. |
02:53 | Check boxes for Command line , Model Panel , Side View. |
02:59 | Scroll down and click on MatchMaker and Match-Align. |
03:05 | You can customize your own tool bar. |
03:08 | Select tools according to your requirement. |
03:12 | Observe the panel. |
03:14 | Icons have been added to the Tool bar on top of the panel. |
03:19 | To change the position of the tool bar, click on the Toolbar placement button. |
03:25 | And select the option from the drop-down menu. |
03:29 | When you finish adding all icons to the tool bar, click on Save button. |
03:35 | And click on Close to close the dialog box. |
03:39 | The structures are now in different positions. |
03:43 | Now, let us superimpose the structures using MatchMaker function. |
03:48 | Click on MatchMaker tool on the Tool bar. |
03:53 | A MatchMaker dialog box opens. |
03:56 | Click on 3w7f as Reference structure. |
04:01 | For now, let us continue with the default settings. Press OK button. |
04:07 | Observe the panel. |
04:09 | The two structures are superimposed on each other. |
04:13 | The two structures are almost completely superimposable with each other |
04:19 | except for a small part which is non-superimposable. |
04:23 | The fragment involved in this non-superimposable part starts from the amino acid number 53 to amino acid number 57. |
04:34 | The MatchMaker generates a sequence alignment using residue types and secondary structure. |
04:40 | And then fits the sequence-aligned residues in 3D. |
04:44 | Let us now check the Match-Align tool. |
04:48 | Click on Match-Align tool from the tool bar. A dialog box opens. |
04:55 | To select the chains, click on their pdb Ids. |
04:59 | Click on OK button. |
05:03 | A sequence alignment dialog box opens. |
05:05 | In this box, the amino acid pairs used in the final fit, are shown with light orange boxes. |
05:13 | The structures are mostly similar except for the loop at residues 52-54. |
05:21 | Place the cursor over the corresponding one letter code. |
05:25 | The residues from 52 to 54 are shifted due to the presence of gaps in the sequence. |
05:33 | Select this part using the cursor. |
05:38 | Observe the panel. |
05:41 | The part of the sequence selected is highlighted. |
05:45 | This corresponds to the non-superimposable part of the structure. |
05:50 | You can change the color of this non-superimposing parts using Actions menu. |
05:55 | Click on Actions menu. |
05:57 | Scroll down to Color, click on orange-red option. |
06:02 | Now this part is highlighted. |
06:05 | Clear the selection and close the dialog box. |
06:10 | Now, let me demonstrate how to morph conformations and create a trajectory. |
06:15 | Morphing involves calculating a series of intermediate structures between the original input structures. |
06:23 | The series of intermediate structures created can be saved as Molecular Dynamics Movie.- in short MD Movie. |
06:32 | Back to the panel. |
06:34 | Start the morphing tool from the Tools menu. |
06:37 | Click on Tools menu scroll down to Structure Comparison. |
06:42 | Click on Morph conformations option. |
06:46 | A Morph conformations dialog box opens. |
06:50 | Click on Add. |
06:52 | In the resulting list of the models dialog box; double click on model number zero, that is 3w7f to add to the conformations. |
07:03 | Next click on model number 1 that is 2zco. |
07:10 | Then again click on 3w7f, that is model number zero. |
07:16 | This sequence corresponds to : a morph trajectory from ligand , bound to the structure to empty structure , and then back. |
07:26 | Close the model list dialog. |
07:29 | Click on Create in the Morph conformations dialog box. |
07:34 | After a few seconds a MD Movie is created. |
07:39 | The dialog box appears on the screen. |
07:42 | The dialog box has buttons to play or pause the movie. |
07:46 | Click on the arrow button to play. |
07:50 | Observe the panel. |
07:52 | The morphing of conformations is being played as a movie. |
07:57 | Pause the movie and close the MD movie dialog box. |
08:02 | Now let's summarize what we have learnt. |
08:05 | In this tutorial we have learnt to, Superimpose and compare different structures of the same protein. |
08:12 | Morph conformations and create a trajectory. |
08:16 | Save the trajectory as Molecular Dynamics Movie. |
08:20 | For the assignment, Open the structures of GTP binding proteins with the PDB code: 1TAG and 1TND |
08:31 | Superimpose the structures using MatchMaker tool. |
08:34 | Using Sequence Alignment tool, find out the regions that are non-identical. |
08:41 | The video at the following link summarizes the Spoken Tutorial project. Please download and watch it. |
08:48 | The Spoken Tutorial Project Team conducts workshops and gives certificates for those who pass an online test. For more details, please write to us. |
08:57 | Spoken Tutorial Project is funded by NMEICT, MHRD, Government of India. More information on this Mission is available at the link shown |
09:09 | This is Snehalatha from IIT Bombay signing off. Thank you for joining. |