Difference between revisions of "Biopython/C2/Introduction-to-Biopython/Khasi"

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{| Border=1
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{| border=1
! <center>Time</center>
+
| '''Time'''
! <center>Narration</center>
+
| '''Narration'''
  
 
|-
 
|-
| 00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
+
|
 +
|00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
  
  
 
|-
 
|-
| 00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka '''Biopython'''
+
|
 +
|00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka '''Biopython'''
  
 
|-
 
|-
 +
|
 
| 00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System
 
| 00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System
  
 
|-
 
|-
 +
|
 
| 00:15 Bad ka '''translation''' ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka '''Biopython'''
 
| 00:15 Bad ka '''translation''' ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka '''Biopython'''
  
 
|-
 
|-
 +
|
 
| 00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka
 
| 00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka
  
 
|-
 
|-
| 00:25 Undergraduate Biochemistry lane Bioinformatics
+
|
 +
|00:25 Undergraduate Biochemistry lane Bioinformatics
  
 
|-
 
|-
| 00:29 bad ka basic ''' Python''' programming
+
|
 +
|00:29 bad ka basic ''' Python''' programming
  
 
|-
 
|-
| 00:31 Peit ia ki ''Python''' tutorial ha ka link bala ai
+
|
 +
|00:31 Peit ia ki ''Python''' tutorial ha ka link bala ai
 +
 
 
|-
 
|-
| 00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * '''Ubuntu OS''' version 12.04
+
|
 +
|00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * '''Ubuntu OS''' version 12.04
  
 
|-
 
|-
 +
|
 
| 00:41 '''Python''' version 2.7.3
 
| 00:41 '''Python''' version 2.7.3
  
 
|-
 
|-
 +
|
 
| 00:44 '''Ipython''' version 0.12.1 bad
 
| 00:44 '''Ipython''' version 0.12.1 bad
  
 
|-
 
|-
 +
|
 
| 00:48 '''Biopython''' version 1.58.
 
| 00:48 '''Biopython''' version 1.58.
  
 
|-
 
|-
|  00:51 Ka '''Biopython''' ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology
+
|   
 +
|00:51 Ka '''Biopython''' ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology
  
 
|-
 
|-
|  00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics
+
|   
 +
|00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics
  
 
|-
 
|-
|  01:03 Ia ki tiar jong ka '''Biopython''' lah ju pyndonkam na ka bynta
+
|   
 +
|01:03 Ia ki tiar jong ka '''Biopython''' lah ju pyndonkam na ka bynta
  
 
|-
 
|-
|  01:05 '''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter
+
|   
 +
|01:05 '''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter
  
  
 
|-
 
|-
|  01:14 Ban download data na ka database websites kum ka '''NCBI''', '''ExPASY''', bad kumta ter ter
+
|   
 +
|01:14 Ban download data na ka database websites kum ka '''NCBI''', '''ExPASY''', bad kumta ter ter
  
 
|-
 
|-
| 01:22
+
|01:22
Ban '''Run''' ïaki '''Bioinformatic algorithm'''s kum ka '''BLAST'''.
+
|Ban '''Run''' ïaki '''Bioinformatic algorithm'''s kum ka '''BLAST'''.
  
 
|-
 
|-
| 01:26 Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih
+
|01:26
 +
| Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih
  
 
|-
 
|-
| 01:31 Kum ka nuksa – ban ioh '''complements''', '''transcription''',''' translation''' bad kumta ter ter  
+
| 01:31  
 +
|Kum ka nuksa – ban ioh '''complements''', '''transcription''',''' translation''' bad kumta ter ter  
  
 
|-
 
|-
| 01:38 Ki code ban peit ia ka alignment
+
| 01:38
 +
|Ki code ban peit ia ka alignment
  
 
|-
 
|-
|  01:40 bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher
+
|  01:40
 +
| bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher
  
 
|-
 
|-
| 01:46 Jingpyntip shaphang ban download
+
| 01:46
 +
| Jingpyntip shaphang ban download
  
 
|-
 
|-
Line 79: Line 102:
  
 
|-
 
|-
| 01:54 Ban tip kham bniah, peit ia ka link ba harum.
+
| 01:54
 +
| Ban tip kham bniah, peit ia ka link ba harum.
  
 
|-
 
|-
| 01:59 Ban install ha ka system '''Linux'''
+
| 01:59
 +
| Ban install ha ka system '''Linux'''
  
 
|-
 
|-
| 02:02 Install ia ka '''Python, Ipython''' bad '''Biopython'''da kaba pyndonkam da ka '''Synaptic Package Manager'''
+
| 02:02
 +
| Install ia ka '''Python, Ipython''' bad '''Biopython'''da kaba pyndonkam da ka '''Synaptic Package Manager'''
  
 
|-
 
|-
| 02:08  Ki software ba donkam kin sa install hi
+
| 02:08
 +
| Ki software ba donkam kin sa install hi
  
 
|-
 
|-
| 02:13   Ngi hap ban install hi ia ki software jong ka '''graphic output'''s bad '''plot'''s
+
| 02:13  
 +
Ngi hap ban install hi ia ki software jong ka '''graphic output'''s bad '''plot'''s
  
 
|-
 
|-
| 02:18  Plie ia ka '''terminal''' da kaba nion lang ia u '''Ctrl, Alt''' bad '''T''' key
+
| 02:18
 +
| Plie ia ka '''terminal''' da kaba nion lang ia u '''Ctrl, Alt''' bad '''T''' key
  
 
|-
 
|-
| 02:24  Nga lah dep install lypa ia ka '''Python, Ipython''' bad '''Biopython''' ha system jong nga
+
| 02:24
 +
| Nga lah dep install lypa ia ka '''Python, Ipython''' bad '''Biopython''' ha system jong nga
  
 
|-
 
|-
| 02:30  Plie ia ka '''Ipython''' interpreter da kaba type "ipython" bad sa nion '''Enter'''
+
| 02:30
 +
| Plie ia ka '''Ipython''' interpreter da kaba type "ipython" bad sa nion '''Enter'''
  
 
|-
 
|-
| 02:35   Ka '''IPython'''prompt ka paw ha screen
+
| 02:35
 +
|Ka '''IPython'''prompt ka paw ha screen
  
 
|-
 
|-
| 02:38  Ban pyntikna ba lah install ia ka'''Biopython''', ha ka prompt, type "import Bio",  bad sa press '''Enter'''
+
| 02:38   
 +
|Ban pyntikna ba lah install ia ka'''Biopython''', ha ka prompt, type "import Bio",  bad sa press '''Enter'''
  
 
|-
 
|-
| 02:48 Lada phim ioh message error ei ei, ka mut ka '''Biopython''' lah install
+
| 02:48  
 +
| Lada phim ioh message error ei ei, ka mut ka '''Biopython''' lah install
  
 
|-
 
|-
| 02:54 Hangne, to ngan pynkynmaw ia phi ba ka'''Python''' language ka long case sensitive
+
| 02:54  
 +
| Hangne, to ngan pynkynmaw ia phi ba ka'''Python''' language ka long case sensitive
  
 
|-
 
|-
| 02:59 Da phikir bha haba type ki keywords, ki variables lane '''function'''s
+
| 02:59  
 +
| Da phikir bha haba type ki keywords, ki variables lane '''function'''s
  
 
|-
 
|-
| 03:04 Kumba long, u lain haneng, u, “i” ha '''import''' dei u lowercase bad u “B”dei u uppercase ha '''Bio'''
+
| 03:04  
 +
| Kumba long, u lain haneng, u, “i” ha '''import''' dei u lowercase bad u “B”dei u uppercase ha '''Bio'''
  
 
|-
 
|-
| 03:12 Ha kane ka tutorial, ngin pyndonkam da ka '''Biopython'''modules ban pynkylla ia ka '''DNA sequence'''  
+
| 03:12  
 +
| Ha kane ka tutorial, ngin pyndonkam da ka '''Biopython'''modules ban pynkylla ia ka '''DNA sequence'''  
  
 
|-
 
|-
| 03:19 Ki rukom pyndonkam ki long harum
+
| 03:19  
 +
| Ki rukom pyndonkam ki long harum
  
 
|-
 
|-
| 03:22 Nyngkong eh, shna ia ka '''sequence object''' ban code ia ka '''DNA strand'''
+
| 03:22  
 +
| Nyngkong eh, shna ia ka '''sequence object''' ban code ia ka '''DNA strand'''
  
 
|-
 
|-
| 03:27 Nangta, sa ka '''transcription''' ban code ia ka '''DNA''' strand sha ka '''mRNA'''
+
| 03:27  
 +
|Nangta, sa ka '''transcription''' ban code ia ka '''DNA''' strand sha ka '''mRNA'''
  
 
|-
 
|-
| 03:32 Khatduh eh, ''' translation''' jong ka '''mRNA''' sha ka '''protein''' sequence.
+
| 03:32  
 +
| Khatduh eh, ''' translation''' jong ka '''mRNA''' sha ka '''protein''' sequence.
 +
 
 
|-
 
|-
| 03:37  Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa
+
| 03:37   
 +
|Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa
  
 
|-
 
|-
| 03:42 Ka code naka bynta u '''protein''' sequence barit
+
| 03:42  
 +
| Ka code naka bynta u '''protein''' sequence barit
  
 
|-
 
|-
| 03:46 ka synjam ba nyngkong ka dei ban shna ia ka '''sequence object''' na ka bynta ka coding '''DNA''' strand kaba hajrong
+
| 03:46  
 +
| ka synjam ba nyngkong ka dei ban shna ia ka '''sequence object''' na ka bynta ka coding '''DNA''' strand kaba hajrong
  
 
|-
 
|-
| 03:52  To ngin ia phai biang sha ka '''terminal'''
+
| 03:52   
 +
|To ngin ia phai biang sha ka '''terminal'''
  
 
|-
 
|-
| 03:55 Ban shna ia ka sequence object, import ïaka ka '''Seq''' module na ka '''Bio ''' package
+
| 03:55  
 +
|Ban shna ia ka sequence object, import ïaka ka '''Seq''' module na ka '''Bio ''' package
  
 
|-
 
|-
| 04:02   Ka ''' Seq '''module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects
+
| 04:02
 +
| Ka ''' Seq '''module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects
  
 
|-
 
|-
| 04:08  Ha ka prompt, type: '''from Bio dot Seq import Seq ''' bad sa nion '''Enter'''
+
| 04:08   
 +
|Ha ka prompt, type: '''from Bio dot Seq import Seq ''' bad sa nion '''Enter'''
  
 
|-
 
|-
|  04:17 Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka '''sequence object''' jong phi.  
+
|  04:17
 +
| Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka '''sequence object''' jong phi.  
  
 
|-
 
|-
|  04:24 Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka '''nucleotides''' lane '''amino acids'''
+
|  04:24  
 +
|Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka '''nucleotides''' lane '''amino acids'''
  
 
|-
 
|-
| 04:32 Ban leh kumta, ngin pyndonkam da ka '''IUPAC '''module na ka '''Alphabet '''package
+
| 04:32  
 +
| Ban leh kumta, ngin pyndonkam da ka '''IUPAC '''module na ka '''Alphabet '''package
  
 
|-
 
|-
| 04:38 Ha ka prompt, type: '''from Bio dot Alphabet import IUPAC'''. Sa nion '''Enter'''
+
| 04:38  
 +
| Ha ka prompt, type: '''from Bio dot Alphabet import IUPAC'''. Sa nion '''Enter'''
  
 
|-
 
|-
| 04:48 Kynmaw ba ngi lah pyndonkam '''import''' bad '''from''' statements ban '''load''' "Seq" bad "IUPAC" modules
+
| 04:48  
 +
|Kynmaw ba ngi lah pyndonkam '''import''' bad '''from''' statements ban '''load''' "Seq" bad "IUPAC" modules
  
 
|-
 
|-
| 04:56  Buh ia u sequence object ha ka variable kaba kyrteng '''cdna'''
+
| 04:56   
 +
|Buh ia u sequence object ha ka variable kaba kyrteng '''cdna'''
  
 
|-
 
|-
| 05:01 Ha ka prompt, type: '''cdna equal to Seq''' kum ha ki normal '''string'''
+
| 05:01  
 +
|Ha ka prompt, type: '''cdna equal to Seq''' kum ha ki normal '''string'''
  
 
|-
 
|-
| 05:08 Pyndait ia ki sequence hapoh ki double quotes bad parentheses
+
| 05:08  
 +
| Pyndait ia ki sequence hapoh ki double quotes bad parentheses
  
 
|-
 
|-
|  05:13  Ngi tip ba ka sequence jong ngi ka dei ka '''DNA''' ba lyngkhot. Te, type '''unambiguous DNA '''alphabet object kum ka '''argument'''
+
|  05:13
 +
| Ngi tip ba ka sequence jong ngi ka dei ka '''DNA''' ba lyngkhot. Te, type '''unambiguous DNA '''alphabet object kum ka '''argument'''
 +
 
 
|-
 
|-
| 05:21  Na ka bynta ka output, type: '''cdna'''. Nion '''Enter'''
+
| 05:21
 +
| Na ka bynta ka output, type: '''cdna'''. Nion '''Enter'''
  
 
|-
 
|-
| 05:26  Ka output ka pyni ia ka '''DNA sequence''' kum ka sequence object
+
| 05:26   
 +
|Ka output ka pyni ia ka '''DNA sequence''' kum ka sequence object
  
 
|-
 
|-
| 05:30 To ngin thoh ia ka coding '''DNA''' strand sha ka ''' mRNA.''' Ba ïadei bad kane.
+
| 05:30  
 +
| To ngin thoh ia ka coding '''DNA''' strand sha ka ''' mRNA.''' Ba ïadei bad kane.
  
 
|-
 
|-
| 05:35   Ngin pyndonkam da ka rukom  '''Seq''' module's built-in “transcribe”'''
+
| 05:35
 +
| Ngin pyndonkam da ka rukom  '''Seq''' module's built-in “transcribe”'''
  
 
|-
 
|-
| 05:39 Type ia ki code kiba harum  
+
| 05:39  
 +
| Type ia ki code kiba harum  
  
 
|-
 
|-
| 05:41 Buh ia ka output ha ka variable '''mrna'''
+
| 05:41  
 +
|Buh ia ka output ha ka variable '''mrna'''
  
 
|-
 
|-
|  05:45 Ha ka prompt, type: '''mrna equal to cdna dot transcribe open and close parentheses''', nion '''Enter'''  
+
|  05:45  
 +
| Ha ka prompt, type: '''mrna equal to cdna dot transcribe open and close parentheses''', nion '''Enter'''  
  
 
|-
 
|-
|  05:55 Na ka bynta ka output, type: ''' mrna.''' Nion '''Enter'''  
+
|  05:55  
 +
|Na ka bynta ka output, type: ''' mrna.''' Nion '''Enter'''  
 +
 
  
 
|-
 
|-
|  06:01 Khmih thuh ia ka output. Ka rukom '''transcribe''' kan bujli ia ka '''Thiamin''' ha ka '''DNA''' sequence da ka '''Uracil'''
+
|  06:01
 +
|Khmih thuh ia ka output. Ka rukom '''transcribe''' kan bujli ia ka '''Thiamin''' ha ka '''DNA''' sequence da ka '''Uracil'''
  
 
|-
 
|-
| 06:09 Kaba bud, ban '''translate''' ia kane ka '''mRNA''' sha ka corresponding '''protein''' sequence pyndonkam da ka rukom jong ka '''translate'''
+
| 06:09  
 +
|Kaba bud, ban '''translate''' ia kane ka '''mRNA''' sha ka corresponding '''protein''' sequence pyndonkam da ka rukom jong ka '''translate'''
  
 
|-
 
|-
| 06:16 Type ia kine ki code harum: '''protein equal to mrna dot translate open and close parentheses'''. Nion '''Enter'''
+
| 06:16  
 +
| Type ia kine ki code harum: '''protein equal to mrna dot translate open and close parentheses'''. Nion '''Enter'''
  
 
|-
 
|-
|  06:27  Ka translate method '''RNA''' lane '''DNA''' sequence da kaba pyndom ia ka standard genetic code
+
|  06:27   
 +
|Ka translate method '''RNA''' lane '''DNA''' sequence da kaba pyndom ia ka standard genetic code
  
 
|-
 
|-
| 06:36 Ka output ka pyni ia ka amino acid sequence.
+
| 06:36  
 +
| Ka output ka pyni ia ka amino acid sequence.
  
 
|-
 
|-
| 06:40 Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka '''stop codons''' ha ka sequence lah pynkylla.  
+
| 06:40  
 +
|Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka '''stop codons''' ha ka sequence lah pynkylla.  
  
 
|-
 
|-
| 06:47 Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka '''stop codon'''.
+
| 06:47  
 +
|Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka '''stop codon'''.
  
 
|-
 
|-
| 06:53   Ha u code ba ha jrong, ngi lah pyndonkam ia u coding '''DNA''' strand na ka bynta ka '''transcription'''
+
| 06:53  
 +
Ha u code ba ha jrong, ngi lah pyndonkam ia u coding '''DNA''' strand na ka bynta ka '''transcription'''
 
    
 
    
 
|-
 
|-
| 06:59 Ha Biopython, ka '''transcribe'''method ka treikam tang ha ka coding DNA strand  
+
| 06:59
 +
| Ha Biopython, ka '''transcribe'''method ka treikam tang ha ka coding DNA strand  
  
 
|-
 
|-
 
| 07:04
 
| 07:04
Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka '''transcription''' ka sdang da ka '''template strand'''
+
|Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka '''transcription''' ka sdang da ka '''template strand'''
  
 
|-
 
|-
| 07:11  Lada phi sdang da ka ''' template strand''', pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka '''reverse complement''' method, kumba lah pyni ha ka terminal.
+
| 07:11   
 +
|Lada phi sdang da ka ''' template strand''', pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka '''reverse complement''' method, kumba lah pyni ha ka terminal.
  
 
|-
 
|-
| 07:20 Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand  
+
| 07:20  
 +
|Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand  
  
 
|-
 
|-
| 07:24 Da kaba pyndonkam da ki rukom ha '''Biopython'''  ngi la dep ban pynkylla ia ka '''DNA''' sequence sha ka '''protein''' sequence.     
+
| 07:24  
 +
| Da kaba pyndonkam da ki rukom ha '''Biopython'''  ngi la dep ban pynkylla ia ka '''DNA''' sequence sha ka '''protein''' sequence.     
  
 
|-
 
|-
| 07:31 Ka '''DNA''' sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code
+
| 07:31
 +
| Ka '''DNA''' sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code
  
 
|-
 
|-
|  07:37 To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang:
+
|  07:37  
 +
|To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang:
  
 
|-
 
|-
| 07:41 Ki jinglong ba kongsan jong ka '''Biopython'''
+
| 07:41
 +
| Ki jinglong ba kongsan jong ka '''Biopython'''
  
 
|-
 
|-
| 07:43 Ka thup jingtip shaphang ka download bad installation ha '''Linux OS'''
+
| 07:43
 +
| Ka thup jingtip shaphang ka download bad installation ha '''Linux OS'''
  
 
|-
 
|-
| 07:48 Ban shna sequence object na ka bynta ka '''DNA''' strand
+
| 07:48
 +
| Ban shna sequence object na ka bynta ka '''DNA''' strand
  
 
|-
 
|-
| 07:52 '''Transcription''' ia ka DNA sequence sha ka '''mRNA'''
+
| 07:52
 +
| '''Transcription''' ia ka DNA sequence sha ka '''mRNA'''
  
 
|-
 
|-
| 07:56 '''Translation''' ia ka '''mRNA''' sha ka protein sequence.  
+
| 07:56
 +
| '''Translation''' ia ka '''mRNA''' sha ka protein sequence.  
  
 
|-
 
|-
| 08:00 Mynta, na ka bynta ka assignment
+
| 08:00
 +
| Mynta, na ka bynta ka assignment
  
 
|-
 
|-
| 08:02 Pynkylla ka '''DNA''' sequence ba lah ai sha ka '''protein''' sequence.  
+
| 08:02  
 +
|Pynkylla ka '''DNA''' sequence ba lah ai sha ka '''protein''' sequence.  
  
 
|-
 
|-
| 08:06 Khmih thuh ia ka output
+
| 08:06
 +
| Khmih thuh ia ka output
  
 
|-
 
|-
|  08:08 Ka protein sequence ka don ia ka '''stop codon'''
+
|  08:08  
 +
|Ka protein sequence ka don ia ka '''stop codon'''
  
 
|-
 
|-
| 08:11 Kumba ka ju long shisha, pynkylla ia ka '''DNA''' shaduh U '''stop codon'''uba nyngkong haka frame
+
| 08:11  
 +
|Kumba ka ju long shisha, pynkylla ia ka '''DNA''' shaduh U '''stop codon'''uba nyngkong haka frame
  
 
|-
 
|-
| 08:17 Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum  
+
| 08:17
 +
| Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum  
  
 
|-
 
|-
| 08:20 Peit thuh ba ngi lah pyndonkam ka argument'''to underscore stop'''  ha ka '''translate''' method. Peit ka output
+
| 08:20  
 +
|Peit thuh ba ngi lah pyndonkam ka argument'''to underscore stop'''  ha ka '''translate''' method. Peit ka output
  
 
|-
 
|-
| 08:27 Ia u '''stop codon''' hi khlem shym la pynkylla
+
| 08:27
 +
| Ia u '''stop codon''' hi khlem shym la pynkylla
  
 
|-
 
|-
| 08:31 U stop symbol u khlem shah kynthup ha kaba kut ka '''protein''' sequence.
+
| 08:31
 +
| U stop symbol u khlem shah kynthup ha kaba kut ka '''protein''' sequence.
  
| 08:36 Kane ka video ka batai lyngkot ia ka Spoken Tutorial project  
+
| 08:36
 +
| Kane ka video ka batai lyngkot ia ka Spoken Tutorial project  
  
 
|-
 
|-
| 08:39 Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka  
+
| 08:39
 +
| Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka  
  
 
|-
 
|-
|08:43  Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test
+
|08:43
 +
|   Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test
  
 
|-
 
|-
| 08:50 Ban tip kham bniah, sngewbha thoh sha ngi
+
| 08:50
 +
| Ban tip kham bniah, sngewbha thoh sha ngi
  
 
|-
 
|-
| 08:53 Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India
+
| 08:53
 +
| Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India
  
 
|-
 
|-
| 08:59  Khambuh ki jingtip halor kane ka mission ka don ha kane ka link
+
| 08:59
 +
| Khambuh ki jingtip halor kane ka mission ka don ha kane ka link
  
 
|-
 
|-
| 09:03 ïa kane ka script la pynkylla sha ka Ktien Khasi da i Yuwanki Yolande Kharlukhi na Shillong, khublei ba phi la ïasnoh lang.|}
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| 09:03  
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|ïa kane ka script la pynkylla sha ka Ktien Khasi da i Yuwanki Yolande Kharlukhi na Shillong, khublei ba phi la ïasnoh lang.|}

Revision as of 15:36, 23 April 2018

Time Narration
00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Introduction to Biopython.


00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka Biopython
00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System
00:15 Bad ka translation ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka Biopython
00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka
00:25 Undergraduate Biochemistry lane Bioinformatics
00:29 bad ka basic Python programming
00:31 Peit ia ki Python' tutorial ha ka link bala ai
00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 12.04
00:41 Python version 2.7.3
00:44 Ipython version 0.12.1 bad
00:48 Biopython version 1.58.
00:51 Ka Biopython ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology
00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics
01:03 Ia ki tiar jong ka Biopython lah ju pyndonkam na ka bynta
01:05 Parsing, kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka FASTA, Genbank, bad kumta ter ter


01:14 Ban download data na ka database websites kum ka NCBI, ExPASY, bad kumta ter ter
01:22 Ban Run ïaki Bioinformatic algorithms kum ka BLAST.
01:26 Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih
01:31 Kum ka nuksa – ban ioh complements, transcription, translation bad kumta ter ter
01:38 Ki code ban peit ia ka alignment
01:40 bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher
01:46 Jingpyntip shaphang ban download
01:48 Ka Biopython kam dei kaba wan lang bad ka Python; ngi hap ban download ïaka laka jong
01:54 Ban tip kham bniah, peit ia ka link ba harum.
01:59 Ban install ha ka system Linux
02:02 Install ia ka Python, Ipython bad Biopythonda kaba pyndonkam da ka Synaptic Package Manager
02:08 Ki software ba donkam kin sa install hi
02:13 Ngi hap ban install hi ia ki software jong ka graphic outputs bad plots
02:18 Plie ia ka terminal da kaba nion lang ia u Ctrl, Alt bad T key
02:24 Nga lah dep install lypa ia ka Python, Ipython bad Biopython ha system jong nga
02:30 Plie ia ka Ipython interpreter da kaba type "ipython" bad sa nion Enter
02:35 Ka IPythonprompt ka paw ha screen
02:38 Ban pyntikna ba lah install ia kaBiopython, ha ka prompt, type "import Bio", bad sa press Enter
02:48 Lada phim ioh message error ei ei, ka mut ka Biopython lah install
02:54 Hangne, to ngan pynkynmaw ia phi ba kaPython language ka long case sensitive
02:59 Da phikir bha haba type ki keywords, ki variables lane functions
03:04 Kumba long, u lain haneng, u, “i” ha import dei u lowercase bad u “B”dei u uppercase ha Bio
03:12 Ha kane ka tutorial, ngin pyndonkam da ka Biopythonmodules ban pynkylla ia ka DNA sequence
03:19 Ki rukom pyndonkam ki long harum
03:22 Nyngkong eh, shna ia ka sequence object ban code ia ka DNA strand
03:27 Nangta, sa ka transcription ban code ia ka DNA strand sha ka mRNA
03:32 Khatduh eh, translation jong ka mRNA sha ka protein sequence.
03:37 Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa
03:42 Ka code naka bynta u protein sequence barit
03:46 ka synjam ba nyngkong ka dei ban shna ia ka sequence object na ka bynta ka coding DNA strand kaba hajrong
03:52 To ngin ia phai biang sha ka terminal
03:55 Ban shna ia ka sequence object, import ïaka ka Seq module na ka Bio package
04:02 Ka Seq module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects
04:08 Ha ka prompt, type: from Bio dot Seq import Seq bad sa nion Enter
04:17 Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka sequence object jong phi.
04:24 Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka nucleotides lane amino acids
04:32 Ban leh kumta, ngin pyndonkam da ka IUPAC module na ka Alphabet package
04:38 Ha ka prompt, type: from Bio dot Alphabet import IUPAC. Sa nion Enter
04:48 Kynmaw ba ngi lah pyndonkam import bad from statements ban load "Seq" bad "IUPAC" modules
04:56 Buh ia u sequence object ha ka variable kaba kyrteng cdna
05:01 Ha ka prompt, type: cdna equal to Seq kum ha ki normal string
05:08 Pyndait ia ki sequence hapoh ki double quotes bad parentheses
05:13 Ngi tip ba ka sequence jong ngi ka dei ka DNA ba lyngkhot. Te, type unambiguous DNA alphabet object kum ka argument
05:21 Na ka bynta ka output, type: cdna. Nion Enter
05:26 Ka output ka pyni ia ka DNA sequence kum ka sequence object
05:30 To ngin thoh ia ka coding DNA strand sha ka mRNA. Ba ïadei bad kane.
05:35 Ngin pyndonkam da ka rukom Seq module's built-in “transcribe”
05:39 Type ia ki code kiba harum
05:41 Buh ia ka output ha ka variable mrna
05:45 Ha ka prompt, type: mrna equal to cdna dot transcribe open and close parentheses, nion Enter
05:55 Na ka bynta ka output, type: mrna. Nion Enter


06:01 Khmih thuh ia ka output. Ka rukom transcribe kan bujli ia ka Thiamin ha ka DNA sequence da ka Uracil
06:09 Kaba bud, ban translate ia kane ka mRNA sha ka corresponding protein sequence pyndonkam da ka rukom jong ka translate
06:16 Type ia kine ki code harum: protein equal to mrna dot translate open and close parentheses. Nion Enter
06:27 Ka translate method RNA lane DNA sequence da kaba pyndom ia ka standard genetic code
06:36 Ka output ka pyni ia ka amino acid sequence.
06:40 Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka stop codons ha ka sequence lah pynkylla.
06:47 Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka stop codon.
06:53 Ha u code ba ha jrong, ngi lah pyndonkam ia u coding DNA strand na ka bynta ka transcription
06:59 Ha Biopython, ka transcribemethod ka treikam tang ha ka coding DNA strand
07:04 Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka transcription ka sdang da ka template strand
07:11 Lada phi sdang da ka template strand, pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka reverse complement method, kumba lah pyni ha ka terminal.
07:20 Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand
07:24 Da kaba pyndonkam da ki rukom ha Biopython ngi la dep ban pynkylla ia ka DNA sequence sha ka protein sequence.
07:31 Ka DNA sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code
07:37 To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang:
07:41 Ki jinglong ba kongsan jong ka Biopython
07:43 Ka thup jingtip shaphang ka download bad installation ha Linux OS
07:48 Ban shna sequence object na ka bynta ka DNA strand
07:52 Transcription ia ka DNA sequence sha ka mRNA
07:56 Translation ia ka mRNA sha ka protein sequence.
08:00 Mynta, na ka bynta ka assignment
08:02 Pynkylla ka DNA sequence ba lah ai sha ka protein sequence.
08:06 Khmih thuh ia ka output
08:08 Ka protein sequence ka don ia ka stop codon
08:11 Kumba ka ju long shisha, pynkylla ia ka DNA shaduh U stop codonuba nyngkong haka frame
08:17 Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum
08:20 Peit thuh ba ngi lah pyndonkam ka argumentto underscore stop ha ka translate method. Peit ka output
08:27 Ia u stop codon hi khlem shym la pynkylla
08:31 U stop symbol u khlem shah kynthup ha kaba kut ka protein sequence. 08:36 Kane ka video ka batai lyngkot ia ka Spoken Tutorial project
08:39 Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka
08:43 Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test
08:50 Ban tip kham bniah, sngewbha thoh sha ngi
08:53 Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India
08:59 Khambuh ki jingtip halor kane ka mission ka don ha kane ka link
09:03 }

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