Difference between revisions of "UCSF-Chimera/C3/Superimposing-and-Morphing/English"
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| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| In this tutorial, we will learn to | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| In this tutorial, we will learn to | ||
− | + | *'''Superimpose''' and compare different structures of the same protein. | |
− | '''Superimpose''' and compare different structures of the same protein. | + | *Morph '''conformations''' and create a '''trajectory'''. |
− | + | ||
− | + | ||
− | Morph '''conformations''' and create a '''trajectory'''. | + | |
− | + | ||
|- | |- | ||
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'''spoken-tutorial.org''' | '''spoken-tutorial.org''' | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| To follow this tutorial you should be familiar with, | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| To follow this tutorial you should be familiar with, '''Chimera''' Interface. |
− | + | ||
− | + | ||
− | '''Chimera''' Interface. | + | |
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| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Here I am using | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Here I am using | ||
− | '''Ubuntu''' OS version. 14.04 | + | *'''Ubuntu''' OS version. 14.04 |
− | + | *'''Chimera''' version 1.10.2 | |
− | '''Chimera''' version 1.10.2 | + | *'''Mozilla firefox''' browser 42.0 |
− | + | *And a working Internet connection. | |
− | '''Mozilla firefox''' browser 42.0 | + | |
− | + | ||
− | And a working Internet connection. | + | |
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Double-click on the Chimera | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Double-click on the Chimera | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Here I have opened a '''Chimera''' window. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Here I have opened a '''Chimera''' window. | ||
− | |||
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on the file '''3w7f '''from the side panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on the file '''3w7f '''from the side panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| From the '''graphic access''' interface, | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| From the '''graphic access''' interface, click on the file '''3w7f'''. |
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The structure of Squalin Synthase opens on the screen. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The structure of '''Squalin Synthase''' opens on the screen. |
Now let us prepare this structure for '''superimposing'''. | Now let us prepare this structure for '''superimposing'''. | ||
− | |||
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| It contains two copies of the same protein. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| It contains two copies of the same '''protein'''. |
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− | + | Press '''Enter'''. | |
|- | |- | ||
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press enter. | press enter. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Next to remove '''solvent '''molecules from the structure | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Next, |
+ | *to remove '''solvent '''molecules from the structure | ||
+ | *I will select the command “'''del solvent'''”. | ||
− | + | Press '''Enter'''. | |
− | + | ||
− | + | ||
|- | |- | ||
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− | We will superimpose two similar proteins to compare their secondary structures. | + | We will '''superimpose''' two similar proteins to compare their secondary structures. |
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|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| On the command line type “'''open 2zco'''” press enter. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| On the command line type “'''open 2zco'''” press enter. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| On the command line type “'''open 2zco'''” press enter. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| On the '''command line''' type “'''open 2zco'''” press enter. |
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|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now these two structures are ready to be superimposed. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now these two structures are ready to be '''superimposed'''. |
|- | |- | ||
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'''Structural alignment''' | '''Structural alignment''' | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| '''Superimposition or Structural alignment''' is a tool to compare two or more protein structures. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| '''Superimposition or Structural alignment''' is a tool to compare two or more '''protein''' structures. |
− | + | ||
− | |||
+ | The alignment is based on their shape and three-dimensional conformation. | ||
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Before we start superimposing, let us place some icons in the tool bar. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Before we start '''superimposing''', let us place some icons in the tool bar. |
|- | |- | ||
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'''Match-Align''' | '''Match-Align''' | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| From the Category drop-down, select '''Tools'''. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| From the '''Category''' drop-down, select '''Tools'''. |
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Check boxes for, | Check boxes for, | ||
− | '''Command line''' | + | *'''Command line''' |
− | + | *'''Model Panel''' | |
− | '''Model Panel''' | + | *'''Side View''' |
− | + | ||
− | '''Side View''' | + | |
Scroll down and click on, | Scroll down and click on, | ||
− | + | *'''MatchMaker ''' | |
− | '''MatchMaker ''' | + | *'''and Match-Align''' |
− | + | ||
− | + | ||
− | '''and Match-Align''' | + | |
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| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Hold the mouse button and drag on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Hold the mouse button and drag on the panel. | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The structures are now in different positions. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The structures are now in different positions. | ||
− | |||
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now let us '''superimpose''' the structures using '''MatchMaker''' function. | |
− | + | ||
− | + | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now let us superimpose the structures using '''MatchMaker''' function. | + | |
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''MatchMaker '''tool on the''' Tool bar'''. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''MatchMaker '''tool on the''' Tool bar'''. | ||
− | |||
− | |||
− | |||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on '''MatchMaker '''tool on the''' Tool bar'''. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on '''MatchMaker '''tool on the''' Tool bar'''. | ||
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Press '''OK '''button. | Press '''OK '''button. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on''' 3w7f '''as Reference structure. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on''' 3w7f '''as '''Reference structure'''. |
− | For now let us continue with the default settings. | + | For now, let us continue with the default settings. |
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|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Observe the panel. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Observe the '''panel'''. |
The two structures are '''superimposed''' on each other. | The two structures are '''superimposed''' on each other. | ||
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− | + | The '''amino acid''' number 53 to '''amino acid''' number 57. | |
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| '''The MatchMaker''' generates a '''sequence alignment''' using residue types and secondary structure. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| '''The MatchMaker''' generates a '''sequence alignment''' using '''residue types''' and secondary structure. |
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− | To select the chains, click on their pdb Ids. | + | To select the chains, click on their '''pdb Ids'''. |
|- | |- | ||
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Cursor on the '''sequence alignment dialog box.''' | Cursor on the '''sequence alignment dialog box.''' | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on OK button. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on '''OK''' button. |
A '''sequence alignment''' dialog box opens. | A '''sequence alignment''' dialog box opens. | ||
− | In this box the '''amino acid '''pairs used in the final fit are shown with light orange boxes. | + | In this box the '''amino acid '''pairs used in the final fit, are shown with light orange boxes. |
|- | |- | ||
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− | This corresponds to the '''non- | + | This corresponds to the '''non-superimposable''' part of the structure. |
+ | |||
You can change the color of this '''non-superimposing''' parts using '''Actions''' menu. | You can change the color of this '''non-superimposing''' parts using '''Actions''' menu. | ||
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|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''Actions''' menu, scroll down to '''Color''', click on '''orange-red '''option. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''Actions''' menu, scroll down to '''Color''', click on '''orange-red '''option. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on '''Actions''' menu | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on '''Actions''' menu. |
+ | |||
+ | Scroll down to '''Color''', click on '''orange-red '''option. | ||
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|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now let me demonstrate how to morph '''conformations''' and create a trajectory. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now, let me demonstrate how to morph '''conformations''' and create a trajectory. |
|- | |- | ||
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'''Morphing''' | '''Morphing''' | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Morphing involves calculating a series of intermediate structures between the original input structures. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| '''Morphing''' involves calculating a series of intermediate structures between the original input structures. |
− | The series of intermediate structures created can be saved as '''Molecular Dynamics Movie '''in short''' MD Movie.''' | + | The series of intermediate structures created can be saved as '''Molecular Dynamics Movie.'''- in short''' MD Movie.''' |
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''Tools menu >> Structure Comparison >> Morph conformations.''' | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''Tools menu >> Structure Comparison >> Morph conformations.''' | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Back to the panel. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Back to the '''panel'''. |
Start the morphing tool from the '''Tools''' menu. | Start the morphing tool from the '''Tools''' menu. | ||
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Click on '''Tools menu scroll down to Structure Comparison.''' | Click on '''Tools menu scroll down to Structure Comparison.''' | ||
− | '''Click on Morph conformations '''option | + | '''Click on Morph conformations '''option. |
|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the Morph conformations dialog box. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the Morph conformations dialog box. | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| A '''Morph conformations''' dialog box opens. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| A '''Morph conformations''' dialog box opens. | ||
− | |||
|- | |- | ||
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In the resulting list of the models dialog box; | In the resulting list of the models dialog box; | ||
− | + | *double click on model number zero, that is '''3w7f''' to add to the conformations. | |
− | double click on model number zero, that is '''3w7f''' to add to the conformations. | + | |
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− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| This sequence corresponds to a morph '''trajectory''' | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| This sequence corresponds to |
− | + | * a morph '''trajectory''' from ligand | |
− | + | * bound to the structure to empty structure | |
− | + | * and then back. | |
|- | |- | ||
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|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''Create''' in the Morph conformations dialog. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Click on '''Create''' in the Morph conformations dialog. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on '''Create''' in the Morph conformations dialog box. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Click on '''Create''' in the '''Morph conformations''' dialog box. |
|- | |- | ||
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|- | |- | ||
| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| Cursor on the panel. | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The morphing of conformations is being played as a movie | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The morphing of '''conformations''' is being played as a movie. |
− | + | ||
− | + | ||
+ | Pause the movie and close the '''MD movie''' dialog box. | ||
|- | |- | ||
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Summary | Summary | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| Now let's summarize what we have learnt. |
In this tutorial we have learnt to, | In this tutorial we have learnt to, | ||
− | + | *'''Superimpose '''and compare different structures of the same protein. | |
− | '''Superimpose '''and compare different structures of the same protein. | + | *Morph '''conformations''' and create a '''trajectory'''. |
− | + | *Save the trajectory as '''Molecular Dynamics Movie.''' | |
− | + | ||
− | Morph '''conformations''' and create a '''trajectory'''. | + | |
− | + | ||
− | + | ||
− | Save the trajectory as '''Molecular Dynamics Movie.''' | + | |
− | + | ||
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| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| For the assignment, | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| For the assignment, | ||
− | + | *Open the structures of '''GTP binding '''proteins with the '''PDB code''': '''1TAG''' and '''1TND''' | |
− | Open the structures of '''GTP binding '''proteins with the '''PDB code''': '''1TAG''' and '''1TND''' | + | *Superimpose the structures using '''MatchMaker''' tool. |
− | + | *Using '''Sequence Alignment''' tool, find out the regions that are non-identical. | |
− | + | ||
− | Superimpose the structures using '''MatchMaker''' tool. | + | |
− | + | ||
− | + | ||
− | Using '''Sequence Alignment''' tool, find out the regions that are non-identical. | + | |
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| style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| '''Slide Number 10''' | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:none;padding:0.097cm;"| '''Slide Number 10''' | ||
− | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The '''Spoken Tutorial Project''' Team conducts workshops and gives certificates for those who pass an online test. | + | | style="background-color:#ffffff;border-top:none;border-bottom:1pt solid #000000;border-left:1pt solid #000000;border-right:1pt solid #000000;padding:0.097cm;"| The '''Spoken Tutorial Project''' Team |
+ | *conducts workshops and | ||
+ | *gives certificates for those who pass an online test. | ||
For more details, please write to us. | For more details, please write to us. |
Revision as of 16:11, 1 July 2016
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Slide Number 1
Title Slide |
Welcome to this tutorial on Superimposing and Morphing |
Slide Number 2
Learning Objectives |
In this tutorial, we will learn to
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Slide Number 3
Pre-requisites
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To follow this tutorial you should be familiar with, Chimera Interface.
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Slide Number 4
System Requirement |
Here I am using
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Double-click on the Chimera | Here I have opened a Chimera window. |
Click on the file 3w7f from the side panel. | From the graphic access interface, click on the file 3w7f. |
Cursor on the panel. | The structure of Squalin Synthase opens on the screen.
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Cursor on the panel. | It contains two copies of the same protein.
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Click on the small back triangle at the end of the command line. | I will recall the command by clicking on the command history. |
Scroll down the command history dialog box.
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From the command history dialog box, select the command “delete:.a”
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Select the command “del solvent” from command history.
press enter. |
Next,
Press Enter. |
Cursor on the panel. | This structure is bound to the substrate analog Farnesyl thiopyrophosphate. FPS in short.
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On the command line type “open 2zco” press enter. | On the command line type “open 2zco” press enter.
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Cursor on the panel. | Now these two structures are ready to be superimposed. |
Slide Number 5
Structural alignment |
Superimposition or Structural alignment is a tool to compare two or more protein structures.
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Cursor on the panel. | Before we start superimposing, let us place some icons in the tool bar. |
Click on Favorites menu >>Add to favorites. | Click on Favorites menu.
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Cursor on preferences dialog box.
Model Panel Side View MatchMaker Match-Align |
From the Category drop-down, select Tools.
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Cursor on the panel. | You can customize your own tool bar.
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Cursor pointing tool bar.
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Observe the panel.
Icons have been added to the Tool bar on top of the panel.
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Click on Save button.
Close the window. |
When you finish adding all icons to the tool bar, click on Save button.
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Hold the mouse button and drag on the panel. | The structures are now in different positions. |
Cursor on the panel. | Now let us superimpose the structures using MatchMaker function. |
Click on MatchMaker tool on the Tool bar. | Click on MatchMaker tool on the Tool bar.
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Cursor on MatchMaker dialog box.
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Click on 3w7f as Reference structure.
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Cursor on the panel. | Observe the panel.
The two structures are superimposed on each other. |
Cursor on the panel. | The two structures are almost completely superimposable with each other.
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Cursor on amino acids 53 and 57. | The fragment involved in this non-superimposable part starts from :
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Cursor on the panel. | The MatchMaker generates a sequence alignment using residue types and secondary structure.
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Click on Match-Align tool.
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Let us now check the Match-Align tool.
A dialog box opens.
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Click on OK button.
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Click on OK button.
A sequence alignment dialog box opens.
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Cursor on the sequence alignment dialog box. | The structures are mostly similar except for the loop at residues 52-54. |
Hover the cursor over one letter code. | Place the cursor over the corresponding one letter code. |
Cursor on the sequence alignment dialog box.
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The residues from 52 to 54 are shifted due to the presence of gaps in the sequence.
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Click on Actions menu, Scroll down to Color.
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Observe the panel.
The part of the sequence selected is highlighted.
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Click on Actions menu, scroll down to Color, click on orange-red option. | Click on Actions menu.
Scroll down to Color, click on orange-red option.
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Cursor on the panel. | Now, let me demonstrate how to morph conformations and create a trajectory. |
Slide Number 6
Morphing |
Morphing involves calculating a series of intermediate structures between the original input structures.
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Click on Tools menu >> Structure Comparison >> Morph conformations. | Back to the panel.
Start the morphing tool from the Tools menu. Click on Tools menu scroll down to Structure Comparison. Click on Morph conformations option. |
Cursor on the Morph conformations dialog box. | A Morph conformations dialog box opens. |
Click on Add button.
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Click on Add.
Then again click on 3w7f, that is model number zero. |
Cursor on the Morph conformations dialog box.
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This sequence corresponds to
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Click on x button. | Close the model list dialog. |
Click on Create in the Morph conformations dialog. | Click on Create in the Morph conformations dialog box. |
Cursor on the dialog box. | After a few seconds a MD Movie is created.
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Click on arrow button. | Click on the arrow button to play.
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Cursor on the panel. | The morphing of conformations is being played as a movie.
Pause the movie and close the MD movie dialog box. |
Slide Number 7
Summary |
Now let's summarize what we have learnt.
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Slide Number 8
Assignment |
For the assignment,
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Slide Number 9
Acknowledgement |
The video at the following link summarizes the Spoken Tutorial project.
Please download and watch it. |
Slide Number 10 | The Spoken Tutorial Project Team
For more details, please write to us. |
Slide number 11 | Spoken Tutorial Project is funded by NMEICT, MHRD, Government of India.
More information on this Mission is available at the link shown |
This is Snehalatha from IIT Bombay signing off. Thank you for joining. |