Difference between revisions of "Biopython/C2/Introduction-to-Biopython/Khasi"

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! <center>Time</center>

! <center>Narration</center>

|00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.

| 00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka '''Biopython'''

| 00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System

| 00:15 Bad ka '''translation''' ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka '''Biopython'''

| 00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka

| 00:25 Undergraduate Biochemistry lane Bioinformatics

| 00:29 bad ka basic ''' Python''' programming

| 00:31 Peit ia ki ''Python''' tutorial ha ka link bala ai

| 00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * '''Ubuntu OS''' version 12.04

| 00:41 '''Python''' version 2.7.3

| 00:44 '''Ipython''' version 0.12.1 bad

| 00:48 '''Biopython''' version 1.58.

| 00:51 Ka '''Biopython''' ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology

| 00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics

| 01:03 Ia ki tiar jong ka '''Biopython''' lah ju pyndonkam na ka bynta

| 01:05 '''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter

 

| 01:14 Ban download data na ka database websites kum ka '''NCBI''', '''ExPASY''', bad kumta ter ter

| 01:22

Ban '''Run''' ïaki '''Bioinformatic algorithm'''s kum ka '''BLAST'''.

| 01:26 Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih

| 01:31 Kum ka nuksa – ban ioh '''complements''', '''transcription''',''' translation''' bad kumta ter ter

| 01:38 Ki code ban peit ia ka alignment

| 01:40 bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher

| 01:46 Jingpyntip shaphang ban download

| 01:48 Ka '''Biopython''' kam dei kaba wan lang bad ka '''Python'''; ngi hap ban download ïaka laka jong

| 01:54 Ban tip kham bniah, peit ia ka link ba harum.

| 01:59 Ban install ha ka system '''Linux'''

| 02:02 Install ia ka '''Python, Ipython''' bad '''Biopython'''da kaba pyndonkam da ka '''Synaptic Package Manager'''

| 02:08 Ki software ba donkam kin sa install hi

| 02:13  Ngi hap ban install hi ia ki software jong ka '''graphic output'''s bad '''plot'''s

| 02:18 Plie ia ka '''terminal''' da kaba nion lang ia u '''Ctrl, Alt''' bad '''T''' key

| 02:24  Nga lah dep install lypa ia ka '''Python, Ipython''' bad '''Biopython''' ha system jong nga

| 02:30 Plie ia ka '''Ipython''' interpreter da kaba type "ipython" bad sa nion '''Enter'''

| 02:35  Ka '''IPython'''prompt ka paw ha screen

| 02:38  Ban pyntikna ba lah install ia ka'''Biopython''', ha ka prompt, type "import Bio",  bad sa press '''Enter'''

| 02:48  Lada phim ioh message error ei ei, ka mut ka '''Biopython''' lah install

| 02:54  Hangne, to ngan pynkynmaw ia phi ba ka'''Python''' language ka long case sensitive

| 02:59  Da phikir bha haba type ki keywords, ki variables lane '''function'''s

| 03:04  Kumba long, u lain haneng, u, “i” ha '''import''' dei u lowercase bad u “B”dei u uppercase ha '''Bio'''

| 03:12  Ha kane ka tutorial, ngin pyndonkam da ka '''Biopython'''modules ban pynkylla ia ka '''DNA sequence'''  

| 03:19  Ki rukom pyndonkam ki long harum

| 03:22  Nyngkong eh, shna ia ka '''sequence object''' ban code ia ka '''DNA strand'''

| 03:27 Nangta, sa ka '''transcription''' ban code ia ka '''DNA''' strand sha ka '''mRNA'''

| 03:32  Khatduh eh, ''' translation''' jong ka '''mRNA''' sha ka '''protein''' sequence.

| 03:42  Ka code naka bynta u '''protein''' sequence barit

| 03:46 ka synjam ba nyngkong ka dei ban shna ia ka '''sequence object''' na ka bynta ka coding '''DNA''' strand kaba hajrong

| 03:52  To ngin ia phai biang sha ka '''terminal'''

| 03:55 Ban shna ia ka sequence object, import ïaka ka '''Seq''' module na ka '''Bio ''' package

| 04:02  Ka ''' Seq '''module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects

| 04:08  Ha ka prompt, type: '''from Bio dot Seq import Seq ''' bad sa nion '''Enter'''

|  04:17 Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka '''sequence object''' jong phi.

| 04:24 Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka '''nucleotides''' lane '''amino acids'''

| 04:32  Ban leh kumta, ngin pyndonkam da ka '''IUPAC '''module na ka '''Alphabet '''package

| 04:38  Ha ka prompt, type: '''from Bio dot Alphabet import IUPAC'''. Sa nion '''Enter'''

| 04:48 Kynmaw ba ngi lah pyndonkam '''import''' bad '''from''' statements ban '''load''' "Seq" bad "IUPAC" modules

| 04:56  Buh ia u sequence object ha ka variable kaba kyrteng '''cdna'''

| 05:01 Ha ka prompt, type: '''cdna equal to Seq''' kum ha ki normal '''string'''

| 05:08 Pyndait ia ki sequence hapoh ki double quotes bad parentheses

| 05:13  Ngi tip ba ka sequence jong ngi ka dei ka '''DNA''' ba lyngkhot. Te, type '''unambiguous DNA '''alphabet object kum ka '''argument'''

|-

| 05:21 Na ka bynta ka output, type: '''cdna'''. Nion '''Enter'''

| 05:26  Ka output ka pyni ia ka '''DNA sequence''' kum ka sequence object

| 05:30 To ngin thoh ia ka coding '''DNA''' strand sha ka ''' mRNA.''' Ba ïadei bad kane.

| 05:35   Ngin pyndonkam da ka rukom  '''Seq''' module's built-in “transcribe”'''

| 05:39 Type ia ki code kiba harum  

| 05:41 Buh ia ka output ha ka variable '''mrna'''

|  05:45 Ha ka prompt, type: '''mrna equal to cdna dot transcribe open and close parentheses''', nion '''Enter'''  

|  05:55 Na ka bynta ka output, type: ''' mrna.''' Nion '''Enter'''  

|  06:01 Khmih thuh ia ka output. Ka rukom '''transcribe''' kan bujli ia ka '''Thiamin''' ha ka '''DNA''' sequence da ka '''Uracil'''

| 06:09 Kaba bud, ban '''translate''' ia kane ka '''mRNA''' sha ka corresponding '''protein''' sequence pyndonkam da ka rukom jong ka '''translate'''

| 06:16 Type ia kine ki code harum: '''protein equal to mrna dot translate open and close parentheses'''. Nion '''Enter'''

|  06:27  Ka translate method '''RNA''' lane '''DNA''' sequence da kaba pyndom ia ka standard genetic code

| 06:36 Ka output ka pyni ia ka amino acid sequence.

| 06:40 Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka '''stop codons''' ha ka sequence lah pynkylla.  

| 06:47 Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka '''stop codon'''.

| 06:53   Ha u code ba ha jrong, ngi lah pyndonkam ia u coding '''DNA''' strand na ka bynta ka '''transcription'''

| 06:59 Ha Biopython, ka '''transcribe'''method ka treikam tang ha ka coding DNA strand  

Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka '''transcription''' ka sdang da ka '''template strand'''

| 07:11  Lada phi sdang da ka ''' template strand''', pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka '''reverse complement''' method, kumba lah pyni ha ka terminal.

| 07:20 Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand  

| 07:24 Da kaba pyndonkam da ki rukom ha '''Biopython'''  ngi la dep ban pynkylla ia ka '''DNA''' sequence sha ka '''protein''' sequence.     

| 07:31 Ka '''DNA''' sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code

|  07:37 To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang:

| 07:41 Ki jinglong ba kongsan jong ka '''Biopython'''

| 07:43 Ka thup jingtip shaphang ka download bad installation ha '''Linux OS'''

| 07:48 Ban shna sequence object na ka bynta ka '''DNA''' strand

| 07:52 '''Transcription''' ia ka DNA sequence sha ka '''mRNA'''

| 07:56 '''Translation''' ia ka '''mRNA''' sha ka protein sequence.  

| 08:00 Mynta, na ka bynta ka assignment

| 08:02 Pynkylla ka '''DNA''' sequence ba lah ai sha ka '''protein''' sequence.  

| 08:06 Khmih thuh ia ka output

|  08:08 Ka protein sequence ka don ia ka '''stop codon'''

| 08:11 Kumba ka ju long shisha, pynkylla ia ka '''DNA''' shaduh U '''stop codon'''uba nyngkong haka frame

| 08:17 Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum  

| 08:20 Peit thuh ba ngi lah pyndonkam ka argument'''to underscore stop'''  ha ka '''translate''' method. Peit ka output

| 08:27 Ia u '''stop codon''' hi khlem shym la pynkylla

| 08:31 U stop symbol u khlem shah kynthup ha kaba kut ka '''protein''' sequence.

| 08:36 Kane ka video ka batai lyngkot ia ka Spoken Tutorial project  

| 08:39 Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka  

|08:43  Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test

| 08:50 Ban tip kham bniah, sngewbha thoh sha ngi

| 08:53 Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India

| 08:59  Khambuh ki jingtip halor kane ka mission ka don ha kane ka link

| 09:03 ïa kane ka script la pynkylla sha ka Ktien Khasi da i Yuwanki Yolande Kharlukhi na Shillong, khublei ba phi la ïasnoh lang.|}