Difference between revisions of "Biopython/C2/Introduction-to-Biopython/Khasi"
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| − | | | + | |00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''. |
Revision as of 15:28, 23 April 2018
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| 00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Introduction to Biopython.
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| 00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka Biopython | |
| 00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System | |
| 00:15 Bad ka translation ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka Biopython | |
| 00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka | |
| 00:25 Undergraduate Biochemistry lane Bioinformatics | |
| 00:29 bad ka basic Python programming | |
| 00:31 Peit ia ki Python' tutorial ha ka link bala ai | |
| 00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 12.04 | |
| 00:41 Python version 2.7.3 | |
| 00:44 Ipython version 0.12.1 bad | |
| 00:48 Biopython version 1.58. | |
| 00:51 Ka Biopython ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology | |
| 00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics | |
| 01:03 Ia ki tiar jong ka Biopython lah ju pyndonkam na ka bynta | |
| 01:05 Parsing, kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka FASTA, Genbank, bad kumta ter ter
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| 01:14 Ban download data na ka database websites kum ka NCBI, ExPASY, bad kumta ter ter | |
01:22
Ban Run ïaki Bioinformatic algorithms kum ka BLAST. | |
| 01:26 Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih | |
| 01:31 Kum ka nuksa – ban ioh complements, transcription, translation bad kumta ter ter | |
| 01:38 Ki code ban peit ia ka alignment | |
| 01:40 bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher | |
| 01:46 Jingpyntip shaphang ban download | |
| 01:48 Ka Biopython kam dei kaba wan lang bad ka Python; ngi hap ban download ïaka laka jong | |
| 01:54 Ban tip kham bniah, peit ia ka link ba harum. | |
| 01:59 Ban install ha ka system Linux | |
| 02:02 Install ia ka Python, Ipython bad Biopythonda kaba pyndonkam da ka Synaptic Package Manager | |
| 02:08 Ki software ba donkam kin sa install hi | |
| 02:13 Ngi hap ban install hi ia ki software jong ka graphic outputs bad plots | |
| 02:18 Plie ia ka terminal da kaba nion lang ia u Ctrl, Alt bad T key | |
| 02:24 Nga lah dep install lypa ia ka Python, Ipython bad Biopython ha system jong nga | |
| 02:30 Plie ia ka Ipython interpreter da kaba type "ipython" bad sa nion Enter | |
| 02:35 Ka IPythonprompt ka paw ha screen | |
| 02:38 Ban pyntikna ba lah install ia kaBiopython, ha ka prompt, type "import Bio", bad sa press Enter | |
| 02:48 Lada phim ioh message error ei ei, ka mut ka Biopython lah install | |
| 02:54 Hangne, to ngan pynkynmaw ia phi ba kaPython language ka long case sensitive | |
| 02:59 Da phikir bha haba type ki keywords, ki variables lane functions | |
| 03:04 Kumba long, u lain haneng, u, “i” ha import dei u lowercase bad u “B”dei u uppercase ha Bio | |
| 03:12 Ha kane ka tutorial, ngin pyndonkam da ka Biopythonmodules ban pynkylla ia ka DNA sequence | |
| 03:19 Ki rukom pyndonkam ki long harum | |
| 03:22 Nyngkong eh, shna ia ka sequence object ban code ia ka DNA strand | |
| 03:27 Nangta, sa ka transcription ban code ia ka DNA strand sha ka mRNA | |
| 03:32 Khatduh eh, translation jong ka mRNA sha ka protein sequence. | |
| 03:37 Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa | |
| 03:42 Ka code naka bynta u protein sequence barit | |
| 03:46 ka synjam ba nyngkong ka dei ban shna ia ka sequence object na ka bynta ka coding DNA strand kaba hajrong | |
| 03:52 To ngin ia phai biang sha ka terminal | |
| 03:55 Ban shna ia ka sequence object, import ïaka ka Seq module na ka Bio package | |
| 04:02 Ka Seq module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects | |
| 04:08 Ha ka prompt, type: from Bio dot Seq import Seq bad sa nion Enter | |
| 04:17 Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka sequence object jong phi. | |
| 04:24 Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka nucleotides lane amino acids | |
| 04:32 Ban leh kumta, ngin pyndonkam da ka IUPAC module na ka Alphabet package | |
| 04:38 Ha ka prompt, type: from Bio dot Alphabet import IUPAC. Sa nion Enter | |
| 04:48 Kynmaw ba ngi lah pyndonkam import bad from statements ban load "Seq" bad "IUPAC" modules | |
| 04:56 Buh ia u sequence object ha ka variable kaba kyrteng cdna | |
| 05:01 Ha ka prompt, type: cdna equal to Seq kum ha ki normal string | |
| 05:08 Pyndait ia ki sequence hapoh ki double quotes bad parentheses | |
| 05:13 Ngi tip ba ka sequence jong ngi ka dei ka DNA ba lyngkhot. Te, type unambiguous DNA alphabet object kum ka argument | |
| 05:21 Na ka bynta ka output, type: cdna. Nion Enter | |
| 05:26 Ka output ka pyni ia ka DNA sequence kum ka sequence object | |
| 05:30 To ngin thoh ia ka coding DNA strand sha ka mRNA. Ba ïadei bad kane. | |
| 05:35 Ngin pyndonkam da ka rukom Seq module's built-in “transcribe” | |
| 05:39 Type ia ki code kiba harum | |
| 05:41 Buh ia ka output ha ka variable mrna | |
| 05:45 Ha ka prompt, type: mrna equal to cdna dot transcribe open and close parentheses, nion Enter | |
| 05:55 Na ka bynta ka output, type: mrna. Nion Enter | |
| 06:01 Khmih thuh ia ka output. Ka rukom transcribe kan bujli ia ka Thiamin ha ka DNA sequence da ka Uracil | |
| 06:09 Kaba bud, ban translate ia kane ka mRNA sha ka corresponding protein sequence pyndonkam da ka rukom jong ka translate | |
| 06:16 Type ia kine ki code harum: protein equal to mrna dot translate open and close parentheses. Nion Enter | |
| 06:27 Ka translate method RNA lane DNA sequence da kaba pyndom ia ka standard genetic code | |
| 06:36 Ka output ka pyni ia ka amino acid sequence. | |
| 06:40 Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka stop codons ha ka sequence lah pynkylla. | |
| 06:47 Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka stop codon. | |
| 06:53 Ha u code ba ha jrong, ngi lah pyndonkam ia u coding DNA strand na ka bynta ka transcription | |
| 06:59 Ha Biopython, ka transcribemethod ka treikam tang ha ka coding DNA strand | |
07:04
Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka transcription ka sdang da ka template strand | |
| 07:11 Lada phi sdang da ka template strand, pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka reverse complement method, kumba lah pyni ha ka terminal. | |
| 07:20 Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand | |
| 07:24 Da kaba pyndonkam da ki rukom ha Biopython ngi la dep ban pynkylla ia ka DNA sequence sha ka protein sequence. | |
| 07:31 Ka DNA sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code | |
| 07:37 To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang: | |
| 07:41 Ki jinglong ba kongsan jong ka Biopython | |
| 07:43 Ka thup jingtip shaphang ka download bad installation ha Linux OS | |
| 07:48 Ban shna sequence object na ka bynta ka DNA strand | |
| 07:52 Transcription ia ka DNA sequence sha ka mRNA | |
| 07:56 Translation ia ka mRNA sha ka protein sequence. | |
| 08:00 Mynta, na ka bynta ka assignment | |
| 08:02 Pynkylla ka DNA sequence ba lah ai sha ka protein sequence. | |
| 08:06 Khmih thuh ia ka output | |
| 08:08 Ka protein sequence ka don ia ka stop codon | |
| 08:11 Kumba ka ju long shisha, pynkylla ia ka DNA shaduh U stop codonuba nyngkong haka frame | |
| 08:17 Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum | |
| 08:20 Peit thuh ba ngi lah pyndonkam ka argumentto underscore stop ha ka translate method. Peit ka output | |
| 08:27 Ia u stop codon hi khlem shym la pynkylla | |
| 08:31 U stop symbol u khlem shah kynthup ha kaba kut ka protein sequence. | 08:36 Kane ka video ka batai lyngkot ia ka Spoken Tutorial project |
| 08:39 Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka | |
| 08:43 Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test | |
| 08:50 Ban tip kham bniah, sngewbha thoh sha ngi | |
| 08:53 Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India | |
| 08:59 Khambuh ki jingtip halor kane ka mission ka don ha kane ka link | |
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