View source for Jmol-Application/C4/Proteins-and-Macromolecules/Khasi

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|'''Time'''
| '''Narration'''

|-
|00:01
| Pdiang sngewbha sha kane ka tutorial halor  '''Proteins and Macromolecules.'''

|-
|00:06
| Ha kane ka tutorial, ngin pule ban:

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|00:09
|'''Load''' ki structure jong ki '''protein''' na ka '''Protein Data Bank (PDB)'''

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|00:13
|Download ki file '.pdb' naka '''PDB''' database

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|00:18
|Display ka secondary structure jong ki protein haki format ba pher ba pher.

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|00:24
|Highlight ki '''hydrogen bonds ''' bad ki '''disulfide bonds'''. 

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|00:29
| Ban bud ïa kane ka tutorial, phi dei ban tip bha shaphang ka rukom treikam naka  '''Jmol Application window.'''

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|00:37
|| Lada em, peit ïa ki tutorial ba kum kane kiba don haka website jong ngi. 

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|00:42
| Ban record ïa kane ka tutorial, nga pyndonkam ka:

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|00:46
|'''Ubuntu '''OS version '''12.04'''

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|00:50
|'''Jmol''' version '''12.2.2'''

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|00:54
|'''Java''' version '''7''' 

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|00:57
|'''Mozilla Firefox Browser''' '''22.0'''.

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|01:02
|'''Structure Analysis''' jong ki biomolecules baheh kum ki - 

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|01:06
|'''Proteins''' bad '''Macromolecules'''

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|01:10
|'''Nucleic acids, DNA''' bad '''RNA'''

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|01:13
|'''Crystal structures''' bad '''polymers''' lah ban leh dakaba pyndonkam ka '''Jmol Application.'''

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|01:19
|Hangne, Nga la plie ka '''Jmol window''' bathymmai.

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|01:24
|ïaki '''3D structure''' jong ki biomolecules lah ban peit dakaba download beit beit naka database. 

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|01:29
|Ban leh kumta, click ha ka '''File '''menu, hiar sha ''' Get PDB.''' 

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|01:36
|KA '''Input''' dialog-box ka mih haka '''screen'''. 

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|01:40
|Ngi dei ban type ia ki saw tylli ki dak '''PDB code''' naka bynta u  '''protein,''' bala thmu haka '''Input''' box. 

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|01:48
|ïa Une u code lah ban ioh naka '''Protein Data Bank (PDB) '''website.

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|01:53
|Kane ka dei ka web-page jong ka  '''Protein Data Bank.'''

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|01:57
|Ka don ïaka thup jingtip shaphang ki biomolecules kum ki '''proteins''' bad  ki '''nucleic acids.'''

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|02:04
|Kum ka nuksa, To ngin pyrshang ban wad ïa u '''PDB code '''naka bynta u  '''Pancreatic Enzyme Insulin '''naka '''PDB '''website.

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|02:13
|Ha u '''search box''', type ïaka kyrteng jong u protein kum "human insulin". Ñion u '''Enter''' key haka keyboard.

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|02:24
|Mynta, haka web-page kaba la pyni, Hiar shapoh.

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|02:28
|KA list jong ki  '''Human Insulin''' structure ba ngi tip ryngkat bad ki '''PDB codes '''ka paw haka screen.

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|02:36
|Kumka nuksa, to ngin jied ''' Human Insulin''' ryngkat u code '''4EX1.'''

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|02:44
|Click ha ka kyrteng jong u protein.

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|02:47
|KA '''window'''ka plie ryngkat bad baroh ki jingbatai jong ka structure.

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|02:52
|Ki thup jingtip kum- 

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|02:54
|'''Primary Citation'''

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|02:56
|'''Molecular Description '''bad

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|02:58
|'''Structure Validation''' ki don ha kane ka page.
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|03:02
|Ngi lah ban '''save''' ïa ki structure jong ki proteins kum ki file '.pdb'  bad peit ïa ki haka '''3D mode''' haka '''Jmol.'''

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|03:12
|Click ha ka link '''Download Files''', ba shong ha khlieh ka mon ha kyndong jong ka '''page'''. 

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|03:20
|Naka drop-down menu, jied ïa u jingjied '''PDB file (gz) '''. 

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|03:28
|KA dialog-box ka plie haka screen. 

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|03:32
|JIed ïa u jingjied '''Save file'''. 

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|03:35
|Click ha u jingshon '''OK'''.

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|03:39
|Ka structure jong u '''protein''' ynsa save kum '''4EX1.pdb.gz''', haka '''Downloads '''folder.

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|03:51
|Kumjuh ruh, phi lah ban download ïaki file '.pdb' ba donkam jong ki proteins bapher bapher bad '''save''' ïaki hala ki jong ki jong ki file. 

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|04:02
|Mynta, to ngin leit pat sha ka '''Jmol''' window ban peit ïa ka '''3D''' structure jong u '''Insulin'''.

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|04:09 
|Lada phi long connect sha ka internet, phi lah ban download beit beit ïa ka structure u protein haka '''Jmol panel'''.
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|04:15
|Type ïaki  saw tylli ki  dak '''PDB code “4EX1” ''' haka text-box bad click ha u jingshon '''OK'''. 

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|04:25
|Lada phim long connect sha ka internet te click ha u icon  '''Open a File''' ha u tool-bar.

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|04:34
|KA dialog-box ka plie ha ka panel.

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|04:38
|ïaid sha ka jaka shong jong ka file '''4EX1.pdb.gz''' . kata. Sha ka '''Downloads '''folder.

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|04:47
|JIed ïaka  '''Downloads''' folder bad click ha u jingshon '''Open '''. 

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|04:52
|JIed ïaka file  '''4EX1.pdb.gz''' bad click ha u jingshon '''Open'''.

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|05:00
|Ka '3D' Structure jong u Insulin ka plie haka screen.

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|05:05
|Ka display balong hi jong u protein haka panel ka dei '''ball and stick.'''

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|05:12
|ïaka model jong u protein haka panel la pyni khlem ki hydrogen atoms.

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|05:17
|Ban pyni ïa u model ryngkat ki hydrogen atoms, plie ïaka  '''modelkit''' menu.

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|05:23
|Hiar sha u jingjied '''add hydrogens''' bad click ha u.

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|05:28
|Mynta la pyni ïa U model ryngkat ki hydrogen atom haka panel. 
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|05:33
|ka  '''protein''' structure la pyni ruh ryngkat ki water molecules.

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|05:38
|Ban buhrieh ïaki water molecules, bud ïaki synjam harum kumba la pyni.

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|05:43
|Nyngkong, plie ïaka pop-up menu bad leit sha '''Select.'''

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|05:48
|Naka sub-menu, Jied '''Hetero''' bad click ha u jingjied '''“All Water'''.

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|05:55
|Plie biang  ïaka pop-up menu, leit sha '''Style''' >> '''Scheme '''bad click ha u jingjied '''CPK spacefill '''.

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|06:05
|Kane kan pynkylla ïa baroh ki water molecules sha ka '''CPK Spacefill '''display.

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|06:11 
|Plie biang ïaka pop-up menu bad leit sha '''Style''', hiar sha '''Atoms '''bad click ha u jingjied '''Off '''.

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|06:22
|Mynta ngi don ka '''Insulin''' structure bakhlem kano  kano ka water molecules ha ka panel.

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|06:27
|Nangta, to ngin pule kumno ban pyni ïa ka structure ba –ar jong u '''protein''' haki format bapher bapher.

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|06:35
|Plie ïaka '''pop-up menu'''. 

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|06:37
|Leit sha u jingjied '''Select '''. 

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|06:39
|Hiar sha u '''Protein '''bad click ha u jingjied ''' All '''.

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|06:44
|Plie biang  ïa ka pop-up menu bad hiar sha '''Style, ''' nangta ''' Scheme.'''

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|06:50
|Ka   sub-menu ka plie bad ki jingjied kum '''CPK Spacefill''', '''Ball and Stick, Sticks, Wireframe, cartoon, trace''' ter ter. 

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|07:02  
|Click ha u jingjied ''' Cartoon '''naka sub-menu.

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|07:07
|Kane ka display ka pyni ïa ka structure ba- ar jong u protein kum '''helices, random coils, strands, sheets '''ter ter.

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|07:17
|Na bynta ki jingjied ba khambun jong ka -

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|07:19
|Plie ïa ka pop-up menu bad hiar sha '''Style,''' nangta sha ''' Structures'''.

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|07:25
|Hangne , ngi iohi shuh shuh ki jingjied ban pyni ïaki structure ba-ar jong u protein.

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|07:31
|Kum ka nuksa, click ha u jingjied '''Strands'''.

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|07:35
|ïa u protein la pyni mynta haka panel kum ki '''Strands'''.

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|07:40
|Ban pynkylla ïaka rong jong ka display: Plie ïaka Pop-up-menu. Hiar sha '''Color'''. Jied '''Atoms''' bad click ha u jingjied  '''Blue'''.

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|07:52
|Khmih ïaka jingkylla rong ha ka panel.

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|07:56
|Ban pynkylla biang ïaka structure sha ka '''Ball-and-stick''' display:

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|07:59
|Plie ïaka pop-up menu, jied '''Style''', nangta '''Scheme''' bad click ha u jingjied  '''Ball and stick '''.

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|08:08
|Ngi lah ruh ban highlight ki '''hydrogen bonds '''bad''' di-sulpfide bonds ''' ha u '''protein model'''.

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|08:14
|Ban display ki '''hydrogen bonds''': Plie ïaka pop-up menu bad Hiar sha u '''Style''' bad nangta sha u jingjied '''Hydrogen Bonds'''.

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|08:25 
|Ki jingjied jong  '''The Hydrogen Bonds''' haka pop-up menu ka don ki jinglong kiba harum:

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|08:30
|'''Calculate''', '''Set Hydrogen Bonds Side Chain''',

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|08:35
|'''Set Hydrogen Bonds in the Backbone''' bad ruh ka don ki jingjied ban pynkylla ïaka jingrben jong ki bond.

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|08:42
|Click ha u jingjied  '''Calculate''' ban pyni ïaki '''hydrogen bonds''' haka model.

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|08:47
|ïa ki  '''hydrogen bonds''' la pyni kum ki dash bajrong haka  rong lieh bad rong saw.

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|08:53
|Ban pynkylla ïaka jingrben jong ki '''hydrogen bonds''', click ha u jingjied ''' 0.10 A '''naka ''' pop-up-menu'''.

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|09:02
|Mynta ngi lah ban iohi ïaka jingrben jong ki ''' hydrogen bonds''' haka panel. 

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|09:06
|Ngi lah ruh ban pynkylla ïaka rong jong ki hydrogen bonds. 

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|09:11
|Naka Pop-up-menu, Hiar sha '''Color''', nangta '''Hydrogen Bonds''', nangta click ha u jingjied  '''orange'''.

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|09:20
|Ngi don ïa baroh ki '''hydrogen bonds''' rong orange haka panel.

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|09:25
|Ki '''Disulfide bonds''' bad '''sulphur''' atoms la pyni haka model, haki rong yellow.

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|09:31
|Ban pynpher ïaki disulfide bonds, plie u jingjied '''disulfide bonds''' haka pop-up menu.

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|09:38
|Click ha ki jing long kiba phi kwah ban pynkylla, kum ki, '''size''', '''color''' ter ter.

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|09:44
|Kumjuh ruh, pyrshang ban plie ïaki file '.pdb' jong ki '''enzymes ''' bapher bad peit ïaki '''3D''' structure jong ki.

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|09:51
| To ngin Batai lyngkot. Ha kane ka tutorial, ngi la pule ban:

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|09:57
|'''Load''' ki structure jong ki protein naka '''Protein Data Bank''' (PDB).

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|10:00
|Download ki file  '.pdb' naka database.

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|10:05
|Peit ïaka '''3D structure''' jong u insulin  pyndonkam u '''PDB code.''' 

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|10:10
|Peit ka '''Protein''' structure ba khlem ki water molecules.

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|10:14
|Display ka secondary structure haki  format bapher bapher.

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|10:17
|Highlight ki  '''hydrogen bonds '''bad '''disulfide bonds.'''

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|10:22
|Kane ka assignment naka bynta jong phi-

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|10:24
|Download ïaka file '.pdb' jong  ka '''Human Hemoglobin''' naka '''PDB '''database.

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|10:31
|Pyni ia ka  secondary structure haka '''cartoon '''display.

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|10:35
|Highlight ki  “'''porphyrin'''” units jong u  protein. 

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|10:39
|Peit naka ling harum naka bynta u '''PDB code.'''

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|10:42
| Peit ïaka video badon ha kane ka URL:
http://spoken-tutorial.org/What_is_a_Spoken_Tutorial

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|10:46
| Ka batai lyngkot ïaka '''Spoken Tutorial''' project.

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|10:50
| Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ïaka.

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|10:55
|Ka Spoken Tutorial Project team: 

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|10:57
| Ka pynlong workshop dakaba pyndonkam ki spoken tutorials.

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|11:01
| Ka ai certificates sha kito kiba pass ïaka on-line test.
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|11:06
| Ban tip kham bniah, sngewbha thoh sha:
'''contact@spoken-tutorial.org''' 

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|11:13
|'''Spoken Tutorial''' project kadei shi bynta jong ka ‘‘‘Talk to a Teacher''' project.

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|11:18
| La kyrshan ïaka daka National Mission on Education Lyngba ka ICT, MHRD, Sorkar India.


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|11:25
| Khambun ka jingtip halor kane ka mission kadon ha kane ka link harum:
'''http://spoken-tutorial.org/NMEICT-Intro'''. 

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|11:31
|ïa kane ka script la pynkylla sha ka ktien Khasi da I Hezekiah Lyngdoh na Shillong. Khublei ba phi la ïasnohlang.
|}