View source for Biopython/C2/Parsing-Data/Khasi

{| Border=1
! <center>Time</center>
! <center>Narration</center>

|-
| 00:01
| Khublei baroh. Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Parsing Data.'''

|-
| 00:06
| Ha kane ka tutorial, ngin pule kumno ban download ia ki '''FASTA''' bad '''GenBank''' files na ka '''NCBI''' database website. 

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| 00:14
| Bad ban '''Parse'''ia ki data files da kaba pyndonkam ia ki '''function'''s  ha ka  '''Sequence Input/Output''' module.

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| 00:19
| Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka undergraduate biochemistry lane bioinformatics. 

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| 00:26
| bad ka basic '''Python''' programming.

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| 00:30
| Peit ia ka '''Python''' tutorials ha ka link ba la ai.

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| 00:34
| Ban record ia kane ka tutorial, nga pyndonkam da ka : * '''Ubuntu OS''' version 14.10

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| 00:40
| '''Python''' version 2.7.8

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| 00:44
| '''Ipython interpretor''' version 2.3.0

|-
| 00:48
| '''Biopython''' version 1.64 bad * '''Mozilla Firefox '''browser 35.0.

|-
| 00:56
| Ki scientific data jong ka biology ju store barabor ia ki ha ka text file kum '''FASTA''', '''GenBank''', '''EMBL''', '''Swiss-Prot''' kumta ter ter.

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| 01:07
| Ia ki data files lah ban download na ka database websites.

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| 01:12
| Plie ia ka website link ba lah ai harum, da uno uno u web browser.

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| 01:17
| Ka web-page ka plie.

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| 01:19
| To ngin download iaka '''FASTA''' bad '''GenBank''' files na ka bynta ka human '''insulin gene'''.

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| 01:25
| Ha ka search box, type: "human insulin", click ha '''Search''' button.

|-
| 01:31
| Ka web-page ka pyni shibun tylli ki flies na ka bynta ka human '''insulin gene'''.

|-
| 01:35
| Ban pyni nuksa, ngan jied 4 tylli ki files kiba kyrteng “Homo sapiens Insulin mRNA”.

|-
| 01:43
| Ngan jied ia ki files ba duna ia ka 500 '''base''' pairs.

|-
| 01:48
| Click ha ka check-box ban jied ia ka file ban download. 

|-
| 01:56
| Wanrah ia u cursor sha “'''Send to'''” option, kaba don ha kyndong khlieh ka mon jong ka page.

|-
| 02:02
| Click ha i selection button barit ba don u khnam ba kdew shapoh, ba don hajan ka “'''Send to'''” button.

|-
| 02:09
| Hapoh ka heading “'''Choose destination'''”, click ha '''File''' option.

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| 02:13
| Phi lah ban '''save''' ia kane ka file ha kano kano ka format, kiba don hapoh '''format''' drop-down list box.

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| 02:21
| Jied '''FASTA''' na ki options ba ai hapoh.

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| 02:25
| Nangta sa click ha '''Create file''' option.

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| 02:29
| Ka dialog-box kan sa mih ha ka screen. 

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|02:32 
| Jied '''Open with''', click ha '''OK.'''

|-
| 02:36
| Ka file ka plie ha ka '''text editor'''.

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| 02:39
| Kane ka file ka pyni 4 tylli ki records, namar ngi la jied 4 tylli ki files ban download. 

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| 02:46
| U line banyngkong ha kawei pa kawei ka record u dei u '''identifier''' line.

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| 02:50
| U sdang da u “greater than >)” symbol.

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| 02:53
| Nangta la pynbud da u'''sequence'''.

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| 02:56
| '''Save''' ia ka file ha '''home'''folder jong phi kum ka“sequence.fasta'”.

|-
| 03:01
| Khang ia ka text editor. 

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| 03:03
| Pynbud ki juh ki syn jam kum haneng ban download ia ki files ha '''GenBank''' format

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| 03:08
| na ka bynta ki files ba la jied hashwa. 

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| 03:12
| Jied ia ka '''file format''' kum '''GenBank.'''

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| 03:16
| Shna ia ka file. Plie da u text editor. 

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| 03:21
| Peit thuh ba ka sequence file ha '''GenBank''' format ka kham bun features ban ia ka '''FASTA''' file.

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| 03:27
| '''Save''' ia ka file kum "sequence.gb" ha ka '''home''' folder. Khang noh u text editor.

|-
| 03:34
| Ban peit nuksa, ngi donkam ia ka FASTA file ba don '''record''' tang iwei. 

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| 03:39
| Na ka bynta kane, pynkhuid ia ki jingjied ba hashwa da kaba click biang ha ki check box.

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| 03:48
| Mynta, jied ia ka file “'''Human insulin gene complete cds'''”.

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| 03:54
| Click ha ka check-box.

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| 03:57
| Bad sa bud ia ki rukom kumba la pyni mynne ban '''save''' ia ka file ha ka '''home''' folder.

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| 04:01
| '''Save''' ia ka file kum "insulin.fasta".

|-
| 04:08
| Ia ki Biological data ba lah store ha kine ki files lah ban sei bad pynkylla da kaba pyndonkam da ka '''Biopython''' libraries. 

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| 04:16
| Khang ia u text-editor. 

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| 04:19
| Ban sei ia ki data na data files la khot '''Parsing'''.

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| 04:23
| Jan ia baroh ki file formats lah ban parsed da kaba pyndonkam '''function'''s kiba don ha '''SeqIO''' module.

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| 04:30
| Ki function jong ka '''SeqIO''' module kiba ju kham pyndonkam bha baroh ki long: '''parse, read, write''' bad '''convert'''.

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| 04:38
| Plie ia ka terminal da kaba nion lang ia '''Ctrl, Alt''' bad '''t''' keys. 

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| 04:44
| Plie ia ka '''Ipython''' da kaba type "ipython" ha ka prompt. Nion '''Enter'''.

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| 04:51
| Nangta, '''import''' "SeqIO" module na '''Bio''' package.

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| 04:56
| Ha ka prompt, type: '''from Bio import SeqIO'''. Nion '''Enter'''.

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| 05:04
| Ngin ia sdang da ka function kaba kham donkam ka “'''parse'''”.

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|05:07
| Na ka bynta ka nuksa, ngan pyndonkam ka '''FASTA''' file ka ba don bun '''record'''s kaba ngi download hashwa na ka database. 

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| 05:17
| Na ba bynta ka '''FASTA''' parsing ba kham suk, type kumne harum ha ka prompt. 

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| 05:22
| Hangne, ngi pyndonkam ia ka '''parse''' function ban read ia ki contents jong ka '''sequence.fasta''' file.

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| 05:30
| Na ka bynta ka output, print '''record id''', sequence kaba don ha ka record bad ruh ia ka jingjrong jong ka sequence. 

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| 05:41
| Peit bha ruh ba ia ka '''parse''' function la pyndonkam ban read sequence data kum '''Sequence record objects'''.

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| 05:48
| La ju pyndonkam barabor bad ka '''for''' loop. 

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| 05:52
| Ka pdiang ar tylli ki '''arguments''', kaba nyngkong dei ka file name ban read ia ka data. 

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| 05:59
| Ka ba ar ka batai shai ia ka format jong ka file. 

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| 06:02
| Nion ia u '''Enter''' key ar sien ban ioh ia ka output. 

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| 06:07
| Ka output ka pyni ia u ''' identifier line, nangta bud sa u sequence uba don ha ka file, bad ruh ka jingjrong jong ka sequence na ka bynta baroh ki records ha ka file. 

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| 06:21
| Peit thuh ba ka '''FASTA''' format kam batai shai ia u alphabet. 

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| 06:26
| Te, ka output kan nym pyni ia ka kum ka '''DNA sequence'''.

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| 06:31
| Ki juh ki synjam lah ban leh biang ban parse iaka '''GenBank''' file.

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| 06:36
| Ban pyni nuksa ngin pyndonkam ia ka '''GenBank''' file kaba ngi lah download mynne na ka database. 

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| 06:43
| Nion ia u up-arrow key ban ioh ia ki lines jong ki code kiba ngi lah pyndonkam hashwa.

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| 06:49
| Pynkylla ia ka kyrteng jong ka file sha ka '''sequence.gb '''.

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| 06:53
| Pynkylla ia ka file format sha ka '''genbank.'''

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| 06:56
| Ki code ba sah kin neh kumjuh. 

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| 06:58
| Nion ia u '''Enter''' key arsien ban ioh ia ka output. 

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| 07:03
| Hangne ruh ka output ka pyni ia ka '''record id''', '''sequence''' bad ka jingjrong jong ka sequence na ka bynta baroh ki records ha ka file. 

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| 07:12
| Peit thuh ba ka '''GenBank''' format ka pyntikna ia ka sequence kum ka DNA sequence.

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| 07:19
| Kumjuh ruh, ia ki '''Swiss-prot''' bad ki file '''EMBL''' lah ban parse da kaba pyndonkam ia u juh u code kum haneng. 

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| 07:27
| Lada ka file jong phi ka don uwei u record phi hap type ia ki line harum ban leh '''parsing'''. 

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| 07:34
| Hangne, ngin pyndonkam iaka  '''FASTA''' file ba lah save mynne, ba tang uwei u record, uta u dei '''insulin.fasta ''' kum ka nuksa. 

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| 07:43
| Peit thuh ba ngi lah dep pyndonkam ia ka '''read''' function ha jaka jong '''parse''' function. Nion '''Enter'''.

|-
| 07:50
| Ka output ka pyni ki contents na ka bynta ka file '''insulin.fasta'''.

|-
| 07:55
| Ka pyni ia ka sequence kum '''sequence record object'''.

|-
| 07:59
| Bad kiwei ki jinglong kum '''GI, accession number '''bad '''description'''.

|-
| 08:06
| Ngi lah ruh ban peit ia ki jinglong ba shimet jong kane ka record kumne harum.

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| 08:11
| Ha ka prompt, type: '''record dot seq'''. Nion '''Enter'''.

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| 08:18
| Ka output ka pyni ia ka sequence ba don ha ka file. 

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| 08:22
| Ban peit ia ki identifiers jong kane ka record, type: '''record dot id. Nion '''Enter'''.

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| 08:29
| Ka output ka pyni ia u '''GI''' number bad accession number bad kumta ter ter. 

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| 08:34
| Phi lah ban pyndonkam ia u function ba lah ong haneng ban '''parse''' ia ki data files kiba phi kwah. 

|-
| 08:40
| Mynta to ngin batai lyngkot).

|-
| 08:42
| Ha kane ka tutorial, ngi lah pule ban: download'''FASTA''' bad '''GenBank''' files na ka '''NCBI''' database website bad pyndonkam iaka  '''parse''' bad '''read''' functions na ka '''SeqIO''' module.

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| 08:55
| Ban sei ia ki data kum ki  '''record id'''s, description bad sequences na '''FASTA''' bad '''GenBank''' files.

|-
| 09:03
| Mynta, na ka bynta ka assignment-

|-
| 09:06
| Download ia ki '''FASTA''' files na ka bynta ka nucleotide sequence haka jingjied jong phi na '''NCBI''' database.

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| 09:13
| Pynkylla ia ki file jong ki sequences sha ki '''reverse complement'''s jong ki.

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| 09:17
| Ka assignment ba lah dep jong phi ka dei ban don ki lines of code kumne harum.

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| 09:22
| Pyndonkam ka  '''parse''' function ban '''load''' ia nucleotide sequences na ka '''FASTA''' file.

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| 09:28
| Nangta, print ia ka reverse complements da kaba pyndonkam ia ka Sequence object’s built in '''reverse complement''' method.

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| 09:37
| Ka video ha ka link harum ka batai lyngkot ia kane ka spoken-tutorial project.

|-
| 09:42
| Sngewbha download bad peit ia ka. 

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| 09:44
| Ka Spoken Tutorial Project team ka ju pynlong ia ki workshops bad ai ruh ia ki certificates sha kito ba pass ia ka on-line test.

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| 09:51
| Ban tip kham bniah, sngewbha thoh sha ngi.

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| 09:55
| Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Sorkar India.

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| 10:01
| Khambun ki jingtip halor kane ka mission ka don ka ha link harum.

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| 10:06
|ïa kane ka script la pynkylla sha ka Ktien Khasi da u Yuwanki Kharlukhi na Shillong,bad ma nga u Hezekiah Lyngdoh ngan pynkut noh. khublei ba phi la ïasnoh lang. 
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