View source for Biopython/C2/Introduction-to-Biopython/Khasi


{| border=1
| '''Time'''
| '''Narration'''

|- 
|00:01 
| Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.

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|00:05 
|Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka '''Biopython'''

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|00:10
|Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System

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| 00:15
| Bad ka '''translation''' ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka '''Biopython'''

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| 00:22
| Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka

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|00:25
| Undergraduate Biochemistry lane Bioinformatics

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|00:29
| bad ka basic ''' Python''' programming

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|00:31
| Peit ia ki ''Python''' tutorial ha ka link bala ai

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|00:35
| Ban record ia kane ka tutorial, nga pyndonkam da ka * '''Ubuntu OS''' version 12.04

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| 00:41 
|'''Python''' version 2.7.3

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| 00:44
| '''Ipython''' version 0.12.1 bad

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| 00:48
| '''Biopython''' version 1.58.

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|00:51
| Ka '''Biopython''' ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology

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|00:57
| ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics

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|01:03
| Ia ki tiar jong ka '''Biopython''' lah ju pyndonkam na ka bynta

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|01:05
| '''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter

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|01:14 
|Ban download data na ka database websites kum ka '''NCBI''', '''ExPASY''', bad kumta ter ter

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|01:22
|Ban '''Run''' ïaki '''Bioinformatic algorithm'''s kum ka '''BLAST'''.

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|01:26
| Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih

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| 01:31 
|Kum ka nuksa – ban ioh '''complements''', '''transcription''',''' translation''' bad kumta ter ter 

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| 01:38
|Ki code ban peit ia ka alignment

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|  01:40
| bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher

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| 01:46
| Jingpyntip shaphang ban download

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| 01:48 
|Ka '''Biopython''' kam dei kaba wan lang bad ka '''Python'''; ngi hap ban download ïaka laka jong 

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| 01:54
| Ban tip kham bniah, peit ia ka link ba harum.

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| 01:59
| Ban install ha ka system '''Linux'''

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| 02:02
| Install ia ka '''Python, Ipython''' bad '''Biopython'''da kaba pyndonkam da ka '''Synaptic Package Manager'''

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| 02:08
|  Ki software ba donkam kin sa install hi

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| 02:13 
|  Ngi hap ban install hi ia ki software jong ka '''graphic output'''s bad '''plot'''s

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| 02:18
|  Plie ia ka '''terminal''' da kaba nion lang ia u '''Ctrl, Alt''' bad '''T''' key

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| 02:24
|  Nga lah dep install lypa ia ka '''Python, Ipython''' bad '''Biopython''' ha system jong nga

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| 02:30
|  Plie ia ka '''Ipython''' interpreter da kaba type "ipython" bad sa nion '''Enter'''

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| 02:35
|Ka '''IPython'''prompt ka paw ha screen

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| 02:38  
|Ban pyntikna ba lah install ia ka'''Biopython''', ha ka prompt, type "import Bio",  bad sa press '''Enter'''

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| 02:48 
| Lada phim ioh message error ei ei, ka mut ka '''Biopython''' lah install

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| 02:54 
| Hangne, to ngan pynkynmaw ia phi ba ka'''Python''' language ka long case sensitive

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| 02:59 
| Da phikir bha haba type ki keywords, ki variables lane '''function'''s

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| 03:04 
| Kumba long, u lain haneng, u, “i” ha '''import''' dei u lowercase bad u “B”dei u uppercase ha '''Bio'''

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| 03:12 
| Ha kane ka tutorial, ngin pyndonkam da ka '''Biopython'''modules ban pynkylla ia ka '''DNA sequence''' 

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| 03:19 
| Ki rukom pyndonkam ki long harum

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| 03:22 
| Nyngkong eh, shna ia ka '''sequence object''' ban code ia ka '''DNA strand'''

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| 03:27 
|Nangta, sa ka '''transcription''' ban code ia ka '''DNA''' strand sha ka '''mRNA'''

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| 03:32 
| Khatduh eh, ''' translation''' jong ka '''mRNA''' sha ka '''protein''' sequence.

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| 03:37  
|Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa

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| 03:42 
| Ka code naka bynta u '''protein''' sequence barit

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| 03:46 
| ka synjam ba nyngkong ka dei ban shna ia ka '''sequence object''' na ka bynta ka coding '''DNA''' strand kaba hajrong

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| 03:52  
|To ngin ia phai biang sha ka '''terminal'''

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| 03:55 
|Ban shna ia ka sequence object, import ïaka ka '''Seq''' module na ka '''Bio ''' package

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| 04:02  
| Ka ''' Seq '''module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects

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| 04:08  
|Ha ka prompt, type: '''from Bio dot Seq import Seq ''' bad sa nion '''Enter'''

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|  04:17
| Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka '''sequence object''' jong phi. 

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|  04:24 
|Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka '''nucleotides''' lane '''amino acids'''

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| 04:32 
| Ban leh kumta, ngin pyndonkam da ka '''IUPAC '''module na ka '''Alphabet '''package

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| 04:38 
| Ha ka prompt, type: '''from Bio dot Alphabet import IUPAC'''. Sa nion '''Enter'''

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| 04:48 
|Kynmaw ba ngi lah pyndonkam '''import''' bad '''from''' statements ban '''load''' "Seq" bad "IUPAC" modules

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| 04:56  
|Buh ia u sequence object ha ka variable kaba kyrteng '''cdna'''

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| 05:01 
|Ha ka prompt, type: '''cdna equal to Seq''' kum ha ki normal '''string'''

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| 05:08 
| Pyndait ia ki sequence hapoh ki double quotes bad parentheses

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|  05:13
|  Ngi tip ba ka sequence jong ngi ka dei ka '''DNA''' ba lyngkhot. Te, type '''unambiguous DNA '''alphabet object kum ka '''argument'''

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| 05:21
|  Na ka bynta ka output, type: '''cdna'''. Nion '''Enter'''

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| 05:26  
|Ka output ka pyni ia ka '''DNA sequence''' kum ka sequence object

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| 05:30 
| To ngin thoh ia ka coding '''DNA''' strand sha ka ''' mRNA.''' Ba ïadei bad kane.

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| 05:35  
| Ngin pyndonkam da ka rukom  '''Seq''' module's built-in “transcribe”'''

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| 05:39 
| Type ia ki code kiba harum 

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| 05:41 
|Buh ia ka output ha ka variable '''mrna'''

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|  05:45 
| Ha ka prompt, type: '''mrna equal to cdna dot transcribe open and close parentheses''', nion '''Enter''' 

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|  05:55 
| Na ka bynta ka output, type: ''' mrna.''' Nion '''Enter''' 

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|  06:01
|Khmih thuh ia ka output. Ka rukom '''transcribe''' kan bujli ia ka '''Thiamin''' ha ka '''DNA''' sequence da ka '''Uracil'''

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| 06:09 
|Kaba bud, ban '''translate''' ia kane ka '''mRNA''' sha ka corresponding '''protein''' sequence pyndonkam da ka rukom jong ka '''translate'''

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| 06:16 
| Type ia kine ki code harum: '''protein equal to mrna dot translate open and close parentheses'''. Nion '''Enter'''

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|  06:27  
|Ka translate method '''RNA''' lane '''DNA''' sequence da kaba pyndom ia ka standard genetic code

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| 06:36 
| Ka output ka pyni ia ka amino acid sequence.

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| 06:40 
|Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka '''stop codons''' ha ka sequence lah pynkylla. 

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| 06:47 
|Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka '''stop codon'''.

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| 06:53 
|  Ha u code ba ha jrong, ngi lah pyndonkam ia u coding '''DNA''' strand na ka bynta ka '''transcription'''
  
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| 06:59
| Ha Biopython, ka '''transcribe'''method ka treikam tang ha ka coding DNA strand 

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| 07:04
|Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka '''transcription''' ka sdang da ka '''template strand'''

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| 07:11  
|Lada phi sdang da ka ''' template strand''', pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka '''reverse complement''' method, kumba lah pyni ha ka terminal.

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| 07:20 
|Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand 

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| 07:24 
| Da kaba pyndonkam da ki rukom ha '''Biopython'''  ngi la dep ban pynkylla ia ka '''DNA''' sequence sha ka '''protein''' sequence.    

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| 07:31
| Ka '''DNA''' sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code

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|  07:37 
|To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang:

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| 07:41
| Ki jinglong ba kongsan jong ka '''Biopython'''

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| 07:43
| Ka thup jingtip shaphang ka download bad installation ha '''Linux OS'''

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| 07:48
| Ban shna sequence object na ka bynta ka '''DNA''' strand

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| 07:52
| '''Transcription''' ia ka DNA sequence sha ka '''mRNA'''

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| 07:56
| '''Translation''' ia ka '''mRNA''' sha ka protein sequence. 

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| 08:00
| Mynta, na ka bynta ka assignment

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| 08:02 
|Pynkylla ka '''DNA''' sequence ba lah ai sha ka '''protein''' sequence. 

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| 08:06
| Khmih thuh ia ka output

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|  08:08 
|Ka protein sequence ka don ia ka '''stop codon'''

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| 08:11 
|Kumba ka ju long shisha, pynkylla ia ka '''DNA''' shaduh U '''stop codon'''uba nyngkong haka frame

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| 08:17
| Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum 

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| 08:20 
|Peit thuh ba ngi lah pyndonkam ka argument'''to underscore stop'''  ha ka '''translate''' method. Peit ka output

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| 08:27
| Ia u '''stop codon''' hi khlem shym la pynkylla

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| 08:31
| U stop symbol u khlem shah kynthup ha kaba kut ka '''protein''' sequence.

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| 08:36
| Kane ka video ka batai lyngkot ia ka Spoken Tutorial project 

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| 08:39
| Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka 

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|08:43
|   Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test

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| 08:50
| Ban tip kham bniah, sngewbha thoh sha ngi

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| 08:53
| Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India

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| 08:59
|  Khambuh ki jingtip halor kane ka mission ka don ha kane ka link

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| 09:03 
|ïa kane ka script la pynkylla sha ka Ktien Khasi da i Yuwanki Yolande Kharlukhi na Shillong, khublei ba phi la ïasnoh lang.