Biopython/C2/Introduction-to-Biopython/Khasi - Revision history

Hezekiah2016 at 05:40, 29 May 2018

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Hezekiah2016 at 09:59, 23 April 2018

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Hezekiah2016 at 09:59, 23 April 2018

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Hezekiah2016 at 08:18, 4 April 2018

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Hezekiah2016: Created page with " {| Border=1 ! Time ! Narration |- | 00:01 |Welcome to this tutorial on '''Introduction to Biopython'''. (Ngi pdiang sngewbha ia phi sha ka..."

2018-02-02T12:14:04Z

Created page with " {| Border=1 ! <center>Time</center> ! <center>Narration</center> |- | 00:01 |Welcome to this tutorial on '''Introduction to Biopython'''. (Ngi pdiang sngewbha ia phi sha ka..."

New page

{| Border=1
! <center>Time</center>
! <center>Narration</center>

|-
| 00:01
|Welcome to this tutorial on '''Introduction to Biopython'''.
(Ngi pdiang sngewbha ia phi sha kane ka nuksa ia ka '''Introduction to Biopython'''.)

|-
| 00:05
|In this tutorial, we will learn about: * important features of '''Biopython'''
(Ha kane ka nuksa , ngin ia pule shaphang ki jingmyntoi jong ka '''Biopython''')

|-
| 00:10
|Information regarding download and installation on Linux Operating System
(Ki jingtip shaphang ka download bad installation jong ka Linux Operating System)

|-
| 00:15
|And '''translation''' of a DNA sequence to a protein sequence using '''Biopython''' tools.
(Bad ka '''translation''' jong ka DNA sekwen sha ka protein sekwen da kaba pyndonkam da ki tiar (tools) ka '''Biopython''')

|-
| 00:22
|To follow this tutorial, you should be familiar with-
(Ban sngewthuh shuh shuh ia kane ka jingbatai , phi dei ban long kiba shemphang ha ka)

|-
| 00:25
| Undergraduate Biochemistry or Bioinformatics
(Undergraduate Biochemistry lane Bioinformatics)

|-
| 00:29
| and basic''' Python''' programming.
(bad ka basic ''' Python''' programming)

|-
| 00:31
|Refer to the '''Python''' tutorials at the given link.
(Pyndonkam da ka nuksa '''Python''' ba lah ai ha ka link hapoh )
|-
| 00:35
|To record this tutorial, I am using: * '''Ubuntu OS''' version 12.04
(Ban record ia kane ka nuksa , nga pyndonkam da ka * '''Ubuntu OS''' version 12.04)

|-
| 00:41
| '''Python''' version 2.7.3
('''Python''' version 2.7.3)

|-
| 00:44
|'''Ipython''' version 0.12.1 and
('''Ipython''' version 0.12.1 bad)

|-
| 00:48
| '''Biopython''' version 1.58.
('''Biopython''' version 1.58.)

|-
| 00:51
| '''Biopython''' is a collection of modules for computational biology.
(Ka '''Biopython''' ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology)

|-
| 00:57
| It can perform most basic to advanced tasks required for bioinformatics.
(ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics)

|-
| 01:03
|'''Biopython''' tools are used for:
(Ia ki tiar (tools) jong ka '''Biopython''' shait pyndonkam ha ka

|-
| 01:05
|'''Parsing''', that is extracting information from various file formats such as '''FASTA''', '''Genbank''' etc.
('''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter)

|-
| 01:14
| Download data from database websites such as '''NCBI''', '''ExPASY''' etc.
(Ban download data na ka database websites kum ka '''NCBI''', '''ExPASY''', bad kumta ter ter)

|-
| 01:22
| '''Run''' '''Bioinformatic algorithm'''s such as '''BLAST'''.
(Ban '''Run''' ïaki '''Bioinformatic algorithm'''s kum ka '''BLAST'''.)

|-
| 01:26
|It has tools for performing common operations on sequences.
(Ka don ia ki tiar (tools) ban trei ki kam ha ka rukom ba ryntih)

|-
| 01:31
| For example- to obtain '''complements''', '''transcription''',''' translation''' etc.
(Nuksa – ban ioh '''complements''', '''transcription''',''' translation''' bad kumta ter ter)

|-
| 01:38
| Code for dealing with alignments
(Ki code ban peit ia ka alignment)

|-
| 01:40
| and code to split up tasks into separate processes.
(bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher)

|-
| 01:46
|Information regarding download:
(Jingpyntip shaphang ban download)

|-
| 01:48
| '''Biopython''' package is not part of the '''Python''' distribution; it needs to be downloaded independently.
(Ka '''Biopython''' kam dei kaba wan lang bad ka '''Python'''; ngi hap ban download ïaka laka jong)

|-
| 01:54
| For details, refer to the following link.
(Ban tip kham bniah, peit ia ka link ba hapoh )

|-
| 01:59
|Installation on '''Linux''' system:
(Ban install ha ka system '''Linux'''

|-
| 02:02
|Install '''Python, Ipython''' and '''Biopython''' packages using '''Synaptic Package Manager'''.
(Install ia ka '''Python, Ipython''' bad '''Biopython'''da kaba pyndonkam da ka '''Synaptic Package Manager'''

|-
| 02:08
|Prerequisite software will be installed automatically.
(Ki software ba donkam kin sa install hi)

|-
| 02:13
| Additional packages must be installed for '''graphic output'''s and '''plot'''s.
(Ngi hap ban install hi ia ki software jong ka '''graphic output'''s bad '''plot'''s

|-
| 02:18
| Open the '''terminal''' by pressing '''Ctrl, Alt''' and '''T''' keys simultaneously.
(Plie ia ka '''terminal''' da kaba nion sah u '''Ctrl, Alt''' bad '''T''' key)

|-
| 02:24
|I have already installed '''Python, Ipython''' and '''Biopython''' on my system.
(Nga lah dep install lypa ka '''Python, Ipython''' bad '''Biopython''' ha system jong nga)

|-
| 02:30
| Start '''Ipython''' interpreter by typing "ipython" and press '''Enter'''.
(Plie ia ka '''Ipython''' interpreter da kaba type "ipython" bad sa nion '''Enter''')

|-
| 02:35
| '''IPython''' prompt appears on screen.
(Ka '''IPython'''prompt kan sa paw ha screen)

|-
| 02:38
|To check the installation of '''Biopython'''- at the prompt, type: "import Bio", press '''Enter'''.
(Ban pyntikna ba lah install ka '''Biopython''', ha ka prompt, type "import Bio", bad sa press '''Enter''')

|-
| 02:48
| If you don't get any error message, it means '''Biopython''' is installed.
(Lada phim ioh message error ei ei, ka mut ka '''Biopython''' ka lah long )

|-
| 02:54
| Here, let me remind you, '''Python''' language is case sensitive.
(Hangne, ai ba ngan pynkynmaw ba ka '''Python''' language ka long case sensitive)

|-
| 02:59
| Take precaution while typing keywords, variables or '''function'''s.
(Da phikir bha haba type ki keywords, ki variables lane '''function'''s)

|-
| 03:04
| For instance, in the above line “i” in '''import''' is lowercase and “B” is uppercase in '''Bio'''.
(Kumba long, hajrong utei u lain , “i” ha '''import''' dei u lowercase bad u “B”dei u uppercase ha '''Bio''')

|-
| 03:12
|In this tutorial, we will make use of '''Biopython''' modules to translate a '''DNA sequence'''.
(Ha kane ka nuksa , ngin pyndonkam da ka '''Biopython'''modules ban pynkylla ia ka sekwen ka '''DNA''' )

|-
| 03:19
|It involves the following steps.
(Ki rukom pyndonkam ki long harum)

|-
| 03:22
|First, create a '''sequence object''' for coding '''DNA strand'''.
(Nyngkong eh, shna ia ka '''sequence object''' ban code ia ka '''DNA strand''')

|-
| 03:27
|Next, '''transcription''' of coding '''DNA''' strand to '''mRNA'''.
(Nangta, sa ka '''transcription''' ban code ia ka '''DNA''' strand sha ka '''mRNA'''

|-
| 03:32
| Finally,''' translation''' of '''mRNA''' to a '''protein''' sequence.
(Khatduh eh, ''' translation''' jong ka '''mRNA''' sha ka '''protein''' sekwen )

|-
| 03:37
|We will be using the coding DNA strand shown on this slide, as an example.
(Ngin pyndonkam ia ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa)

|-
| 03:42
| It codes for a small '''protein''' sequence.
(Ka pyndonkam code da u '''protein''' sekwen barit

|-
| 03:46
| The first step is to create a '''sequence object''' for the above coding '''DNA''' strand.
(Nyngkong eh ngi dei ban shna ia ka '''sekwen object''' na ka bynta ka coding '''DNA''' strand kaba hajrong)

|-
| 03:52
|Let us go back to the '''terminal'''.
(To ngin ia phai biang sha ka '''terminal''')

|-
| 03:55
|For creating a sequence object, import the '''Seq''' module from '''Bio '''package.
(Ban shna ia ka sekwen object, wanrah ka '''Seq''' module na ka '''Bio ''' package)

|-
| 04:02
| The''' Seq '''module provides methods to store and process sequence objects.
( Ka ''' Seq '''module ka ai jingmyntoi ban pyllang bad ban shna ia ki sekwen objects)

|-
| 04:08
| At the prompt, type: '''from Bio dot Seq import Seq ''' press '''Enter'''.
(Ha ka prompt, type: '''from Bio dot Seq import Seq ''' bad sa nion '''Enter'''

|-
| 04:17
|Next, specify the alphabets in the strand explicitly, when creating your '''sequence object'''.
(Nangta, pyntikna ia ki alphabets ha ka strand lakajong , haba shna ia ka '''sequence object''' j

|-
| 04:24
| That is to specify whether the sequence of alphabets code for '''nucleotides''' or '''amino acids'''.
(Kata ka mut ban pynthikna ia ka sekwen jong ki alphabets code na ka bynta ka '''nucleotides''' lane '''amino acids''')

|-
| 04:32
|To do so, we will use '''IUPAC '''module from '''Alphabet '''package.
(Ban leh ia kane , ngin pyndonkam da ka '''IUPAC '''module na ka '''Alphabet '''package)

|-
| 04:38
| At the prompt, type: '''from Bio dot Alphabet import IUPAC'''. Press '''Enter'''.
(Ha ka prompt, type: '''from Bio dot Alphabet import IUPAC'''. Sa nion '''Enter''')

|-
| 04:48
| Note that we have used '''import''' and '''from''' statements to '''load''' "Seq" and "IUPAC" modules.
(Kynmaw ba ngi lah pyndonkam '''import''' bad '''from''' kyntien (statements) ban '''load''' "Seq" bad "IUPAC" modules)

|-
| 04:56
|Store the sequence object in a variable called '''cdna'''.
(Lum ia ki sekwen object ha ka variable kaba kyrteng '''cdna'''

|-
| 05:01
| At the prompt, type: '''cdna equal to Seq''' as in normal '''string'''s.
(Ha ka prompt, type: '''cdna equal to Seq''' kum kaba normal '''string'''

|-
| 05:08
| Enclose the sequence within double quotes and parentheses.
(Pyndait lang ka sekwen hapoh ki double quotes bad parentheses)

|-
| 05:13
| We know our sequence is a '''DNA''' fragment. So, type: '''unambiguous DNA '''alphabet object as an '''argument'''.
(Ngi tip ba ka sekwen jong ngi ka dei ka '''DNA''' ba lyngkhot (fragment). Te, type '''unambiguous DNA '''alphabet object kum ka '''argument''')
|-
| 05:21
| For the output, type: '''cdna'''. Press '''Enter'''.
(Na ka bynta ka output, type: '''cdna'''. Nion '''Enter''')

|-
| 05:26
| The output shows the '''DNA sequence''' as a sequence object.
(Ka output ka pyni ia ka '''DNA sequence''' kum ka sekwen object)

|-
| 05:30
|Let’s transcribe the coding '''DNA''' strand into the corresponding''' mRNA.'''
(To ngin ia thoh shai (transcribe) ia ka coding '''DNA''' strand sha ka corresponding ''' mRNA.''')

|-
| 05:35
| We will use the '''Seq''' module's built-in '''“transcribe”''' method.
(Ngin pyndonkam da ka rukom '''Seq''' module's built-in “transcribe”''')

|-
| 05:39
|Type the following code:
(Thoh ia ki code kiba harum)

|-
| 05:41
| Store the output in a variable '''mrna'''.
(Lum ia ka output ha ka variable '''mrna''')

|-
| 05:45
| At the prompt, type: '''mrna equal to cdna dot transcribe open and close parentheses''', press '''Enter'''.
(Ha ka prompt, thoh : '''mrna equal to cdna dot transcribe open and close parentheses''', nion '''Enter''')

|-
| 05:55
| For the output, type: ''' mrna.''' Press '''Enter'''.
(Na ka bynta ka output, thoh : ''' mrna.''' Nion '''Enter''')

|-
| 06:01
|Observe the output.The '''transcribe''' method replaces the '''Thiamin''' in the '''DNA''' sequence by '''Uracil'''.
(Peit ia ka output. Ka rukom '''transcribe''' kan bujli ia ka '''Thiamin''' ha ka '''DNA''' sekwen da ka '''Uracil''')

|-
| 06:09
|Next, to '''translate''' this '''mRNA''' to corresponding '''protein''' sequence, use the '''translate''' method.
(Hadien , ban '''translate''' ia kane ka '''mRNA''' sha ka corresponding '''protein''' sekwen , pyndonkam da ka rukom jong ka '''translate''')

|-
| 06:16
|Type the following code: '''protein equal to mrna dot translate open and close parentheses'''. Press '''Enter'''.
(Thoh ia kine ki code : '''protein equal to mrna dot translate open and close parentheses'''. Nion '''Enter''')

|-
| 06:27
| The '''translate''' method translates '''RNA''' or '''DNA''' sequence using the standard genetic code, if unspecified.
(Ka rukom '''translate''' kan translate '''RNA''' lane '''DNA''' sekwen da kaba pyndom ia ka standard genetic code)

|-
| 06:36
|The output shows an amino acid sequence.
(Ka output kan pyni ia ka amino acid sekwen )

|-
| 06:40
| The output also shows information regarding the presence of '''stop codons''' in the translated sequence.
(Ka output kan pyni ruh ia ka jingpyntip ia ka jingdon jong ka '''stop codons''' ha ka sekwen ba lah pykylla (translate)

|-
| 06:47
| Observe the asterisk at the end of the protein sequence. It indicates the '''stop codon'''.
(Peit ia u asterisk ha kaba kut jong ka protein sekwen . Ka pyni ia ka '''stop codon'''.)

|-
| 06:53
|In the above code, we have used a coding '''DNA''' strand for '''transcription'''.
(Ha u code ba ha jrong, ngi lah pyndonkam ia u coding '''DNA''' strand na ka bynta ka '''transcription''')

|-
| 06:59
|In Biopython, '''transcribe''' method works only on coding DNA strand.
(Ha Biopython, ka '''transcribe'''method ka treikam tang ha ka coding DNA strand)
|-
| 07:04
|However, in real biological systems, the process of '''transcription''' starts with a '''template strand'''.
(Hynrei, ha ka biological system ba shisha, ka rukom long jong ka '''transcription''' ka sdang da ka '''template strand''')

|-
| 07:11
|If you are starting with a''' template strand''', convert it to coding strand by using '''reverse complement''' method, as shown on the terminal.
(Lada phi sdang da ka ''' template strand''', pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka '''reverse complement''' method, kumba lah pyni ha ka terminal.)

|-
| 07:20
|Follow the rest of the code as shown above, for the coding strand.
(Leh ia kiba sah ki code kumba lah pyni hajrong, na ka bynta ka coding strand)

|-
| 07:24
|Using methods in '''Biopython''' we have translated a '''DNA''' sequence to a '''protein''' sequence.
(Da kaba pyndonkam da ki rukom ha '''Biopython''' ngi la dep ban pynkylla (translated) ia ka '''DNA''' sekwen sha ka '''protein''' sekwen )

|-
| 07:31
|'''DNA''' sequence of any size can be translated to a protein sequence using this code.
(Ka '''DNA''' sekwen kaba ha kano kano ka size lah ban pynkylla sha ka protein sekwen da kaba pyndonkam da une u code)

|-
| 07:37
|Let's summarize.In this tutorial, we have learnt:
(To ngin ia pynkut . Ha kane ka nuksa , ngi leh shaphang:)

|-
| 07:41
| Important features of '''Biopython'''
(Ki mat ba donkam ka '''Biopython''')

|-
| 07:43
| Information regarding download and installation on '''Linux OS'''
(Jingpyntip shaphang ka download bad installation ha '''Linux OS''')
|-
| 07:48
| Create a sequence object for the given '''DNA''' strand.
(Ban shna sekwen object na ka bynta ka '''DNA''' strand)

|-
| 07:52
| '''Transcription''' of the DNA sequence to '''mRNA'''.
('''Transcription''' jong ka DNA sekwen sha ka '''mRNA''')
|-
| 07:56
| '''Translation''' of '''mRNA''' to protein sequence.
('''Translation''' jong ka '''mRNA''' sha ka protein sekwen )

|-
| 08:00
|Now, for the assignment-
(Mynta, na ka bynta ki kam )

|-
| 08:02
|Translate the given '''DNA''' sequence into '''protein''' sequence.
(Pynkylla ka '''DNA''' sekwen ba lah ai sha ka '''protein''' sekwen )

|-
| 08:06
|Observe the output.
(Peit ia ka output)

|-
| 08:08
|The protein sequence has an internal '''stop codon'''.
(Ka protein sekwen ka don lypa ka '''stop codon''')

|-
| 08:11
|As it happens in nature, translate the '''DNA''' till first in frame '''stop codon'''.
(Kumba ka ju long shisha, pynkylla ia ka '''DNA''' shaduh kaba nyngkong ka frame '''stop codon''')

|-
| 08:17
|Your completed assignment should have the following code.
(Ka kam ba lah dep jong phi ka dei ban don ki code kiba harum)

|-
| 08:20
|Notice that we have used '''to underscore stop''' argument in the '''translate()''' method. Notice the output.
(To khmih bha ba ngi lah pyndonkam ka rukom (argument)'''to underscore stop''' ha ka '''translate()''' method. Peit ka output)
|-
| 08:27
|The '''stop codon''' itself is not translated.
(U '''stop codon''' marwei khlem shah pynkylla)

|-
| 08:31
|The stop symbol is not included at the end of your '''protein''' sequence.
(U symbol sangeh u khlem shah kynthup ha kaba kut ka '''protein''' sekwen )

|-
| 08:36
|This video summarizes the Spoken Tutorial project.
(Kane ka video ka lum kyllum ia ka Spoken Tutorial project)

|-
| 08:39
|If you do not have good bandwidth, you can download and watch it.
(Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka)

|-
|08:43
|The Spoken Tutorial Project team conducts workshops and gives certificates for those who pass an online test.
(Ka Spoken Tutorial Project team ka shait pynlong workshop bad ai ruh ia ki khusnam certificates na ka bynta kito ba pass ia ka online test)

|-
| 08:50
|For more details, please write to us.
(Ban tip kham bniah, sngewbha thoh sha ngi)

|-
| 08:53
|Spoken Tutorial Project is funded by NMEICT, MHRD, Government of India.
(Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India)

|-
| 08:59
|More information on this mission is available at this link.
(Na ka bynta ki jingtip ba kham pura jong kane ka mission phin lap ha kane ka link)

|-
| 09:03
|This is Snehalatha from '''IIT Bombay''', signing off. Thank you for joining.
(Ha kaba kut, Nga dei ka Snehalatha na '''IIT Bombay'''. Khublei shibun
|}

@@ Line 6: / Line 6: @@
 |-
 |00:01
-|Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
+| Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
-−
 |-
@@ Line 68: / Line 67: @@
 |01:05
 | '''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter
 |-
@@ Line 99: / Line 97: @@
 |-
-| 01:48 Ka '''Biopython''' kam dei kaba wan lang bad ka '''Python'''; ngi hap ban download ïaka laka jong
+| 01:48
 |-
@@ Line 271: / Line 270: @@
 |-
 |  05:55
-|Na ka bynta ka output, type: ''' mrna.''' Nion '''Enter'''
+| Na ka bynta ka output, type: ''' mrna.''' Nion '''Enter'''
-−
 |-
@@ Line 390: / Line 388: @@
 | U stop symbol u khlem shah kynthup ha kaba kut ka '''protein''' sequence.
 | 08:36
 | Kane ka video ka batai lyngkot ia ka Spoken Tutorial project
@@ Line 415: / Line 414: @@
 |-
 | 09:03
-|ïa kane ka script la pynkylla sha ka Ktien Khasi da i Yuwanki Yolande Kharlukhi na Shillong, khublei ba phi la ïasnoh lang.|}
+|ïa kane ka script la pynkylla sha ka Ktien Khasi da i Yuwanki Yolande Kharlukhi na Shillong, khublei ba phi la ïasnoh lang.

@@ Line 4: / Line 4: @@
 | '''Narration'''
 |-
 |00:01
-|00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
+|Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
 |-
 |00:05
-|00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka '''Biopython'''
+|Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka '''Biopython'''
 |-
 |00:10
-| 00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System
+|Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System
 |-
 | 00:15
-| 00:15 Bad ka '''translation''' ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka '''Biopython'''
+| Bad ka '''translation''' ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka '''Biopython'''
 |-
 | 00:22
-| 00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka
+| Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka
 |-
 |00:25
-|00:25 Undergraduate Biochemistry lane Bioinformatics
+| Undergraduate Biochemistry lane Bioinformatics
 |-
 |00:29
-|00:29 bad ka basic ''' Python''' programming
+| bad ka basic ''' Python''' programming
 |-
 |00:31
-|00:31 Peit ia ki ''Python''' tutorial ha ka link bala ai
+| Peit ia ki ''Python''' tutorial ha ka link bala ai
 |-
 |00:35
-|00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * '''Ubuntu OS''' version 12.04
+| Ban record ia kane ka tutorial, nga pyndonkam da ka * '''Ubuntu OS''' version 12.04
 |-
 | 00:41
-| 00:41 '''Python''' version 2.7.3
+|'''Python''' version 2.7.3
 |-
 | 00:44
-| 00:44 '''Ipython''' version 0.12.1 bad
+| '''Ipython''' version 0.12.1 bad
 |-
 | 00:48
-| 00:48 '''Biopython''' version 1.58.
+| '''Biopython''' version 1.58.
 |-
 |00:51
-|00:51 Ka '''Biopython''' ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology
+| Ka '''Biopython''' ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology
 |-
 |00:57
-|00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics
+| ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics
 |-
 |01:03
-|01:03 Ia ki tiar jong ka '''Biopython''' lah ju pyndonkam na ka bynta
+| Ia ki tiar jong ka '''Biopython''' lah ju pyndonkam na ka bynta
 |-
 |01:05
-|01:05 '''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter
+| '''Parsing''', kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka '''FASTA''', '''Genbank''', bad kumta ter ter
 |-
 |01:14
-|01:14 Ban download data na ka database websites kum ka '''NCBI''', '''ExPASY''', bad kumta ter ter
+|Ban download data na ka database websites kum ka '''NCBI''', '''ExPASY''', bad kumta ter ter
 |-

@@ Line 5: / Line 5: @@
 |-
 | 00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
 :01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.

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−	\| 00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.	+	\|00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.

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 |  00:01
-|Welcome to this tutorial on '''Introduction to Biopython'''.
+|Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka '''Introduction to Biopython'''.
-(Ngi pdiang sngewbha ia phi sha kane ka nuksa ia  ka '''Introduction to Biopython'''.)
 |-
 | 00:05
-|In this tutorial, we will learn about: * important features of '''Biopython'''
+|Ha kane ka tutorial , ngin pule shaphang ki jinglong ba kongsan  jong ka '''Biopython'''
-(Ha kane ka nuksa , ngin ia pule shaphang ki jingmyntoi  jong ka '''Biopython''')
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