Biopython/C2/Manipulating-Sequences/Khasi

Time	Narration
00:01	Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Manipulating Sequences.
00:06	Ha kane ka tutorial, ngin pyndonkam ki tools ka Biopython: Ban pynmih ia u random DNA sequence
00:13	Phiah ia u DNA sequence ha ki jaka ba lah buh
00:17	Pyndait lang ia artylli ki sequences ban pynlong kawei ka sequence ba thymmai
00:22	Wad ia ka jingjrong jong ka sequence
00:26	Niew ia ki number jong ki base lane ki dkhot jong u string
00:31	Wad ia u base ba la thmu lane bynta jong u string
00:35	Pynkylla ia ka sequence object sha ka mutable sequence object
00:40	Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka undergraduate Biochemistry lane Bioinformatics
00:47	Bad ka basic Python programming
00:51	Lada em, peit ia ki tutorials Python ha ka link ba la ai
00:56	Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 14.10
01:03	Python version 2.7.8
01:07	Ipython interpreter version 2.3.0
01:12	Biopython version 1.64.
01:16	To ngin ia plie iaka terminal bad sdang ia ka ipython interpreter
01:21	Nion lang ia u Ctrl, Alt and t key
01:26	Ha ka prompt, type: "ipython" bad sa nion Enter
01:31	Ka Ipython prompt kan sa mih ha ka screen
01:35	Da kaba pyndonkam ia ka Biopython, ngi lah ban pynmih ia ka sequence object na ka bynta ka random DNA sequence ha kano kano ka jingjrong
01:44	To mynta ngin ia pynmih ia ka sequence object na ka bynta ka DNA sequence kaba 20 bases
01:50	Ha ka prompt, type: "import random", bad nion Enter.
01:56	Nangta, import Seq module na Bio package.
02:01	Bunsien Seq ngi ju pronounce kum seek.
02:06	Ha ka prompt, type: From Bio dot Seq import Seq. Nion Enter.
02:15	Ngin pyndonkam da ka Bio.Alphabet module ban pyntikna ia ki alphabets ha ka DNA sequence
02:22	Type: from Bio dot Alphabet import generic underscore dna. Nion Enter.
02:32	Type ia ki command harum ban shna ia ka sequence object na ka bynta ka randon DNA sequence.
02:38	Buh ia ka sequence ha ka variable dna1
02:42	Sngewbha peit: ha kane ka command, pyndonkam ar tylli ki single quotes ha ka jaka ka double quote. Nion Enter
02:50	Na ka bynta ka output, type: dna1. Nion Enter
02:55	Ka output ka pyni ia ka sequence object na ka bynta ka random DNA sequence.
03:00	Lada phi kwah ka sequence ba thymmai, nion u up-arrow key ban ioh ia ka juh ka command kum haneng. Nion Enter.
03:11	Na ka bynta ka output, type ia ka kyrteng ka variable dna1. Nion Enter
03:17	Ka output ka pyni iaka DNA sequence ba thymmai kaba pher na kaba nyngkong.
03:23	Shaphang ka Sequence Objects
03:25	Ka sequence objects ha ka jingshisha ka long kum ka Python strings.
03:30	Te, bud beit ia ka rukom leh kumba leh ia ka Python strings.
03:35	Ha ka Python, ngi niew ia ki characters ha ka string kaba sdang na 0, ha jaka u 1.
03:41	U character ba nyngkong ha ka sequence u dei u zero.
03:45	Phai biang sha ka terminal.
03:47	Teng teng ngi hap ban trei tang shi bynta jong ka sequence
03:52	Mynta, ngin ia peit kumno ban sei ia ki bynta jong ka string bad buh ia ki kum ki sequence objects.
03:58	Nuksa, ngin slice ia ka DNA sequence ha ki ar bynta.
04:04	Nyngkong, hapdeng ki bases 6 bad 7.
04:08	Kane kan sei ia ka fragment na kaba sdang jong ka sequence sha ka base ba 6 ha ka sequence.
04:15	Ka slice ba ar kan dei hapdeng ka bases 11 bad 12.
04:20	Ka fragment ba ar kan dei na ka base ba 12 shaduh ba kut jong ka sequence.
04:26	Type ia ka command ba harum, ha ka prompt, ban sei ia ka fragment ba nyngkong.
04:31	String1 equal to dna1 within brackets 0 semi-colon 6.
04:39	string1 ka dei ka variable ban lum ia ka fragment ba nyngkong.
04:43	Ki command ba bud kin long beit kum ha ka Python.
04:47	Hapoh kine ki brackets dei ki jaka ba sdang bad ba sangeh ba ba la pyniakhlad da u colon.
04:53	Kine ki jaka ki kynthup lang ia kaba sdang hynrei khlem kynthup ïaki ha ka jaka ba sangeh. Nion Enter.
05:01	Ban peit ia ka output, type: "string1", nion Enter.
05:04	Ka output ka pyni ia ka fragment ba nyngkong kum ka sequence object.
05:10	Ban sei ia ka string ba ar na ka sequence, nion ia u up-arrow key bad edit ia ka command kumne harum:
05:17	Pynkylla ia ka ka kyrteng jong u variable sha ka string2 bad jaka sha u 11 bad 20.
05:24	Na ka bynta ka output, type: "string2". Nion Enter.
05:30	Mynta ngi ioh ia ka fragment ba ar ruh kum ka sequence object.
05:34	To ngin pynlong ïaki kawei, kata, ban pyndait lang ia baroh ar ki strings ban pynlong u fragment ba thymmai.
05:42	Buh ia ka sequence ba thymmai ha ka variable dna2.
05:46	Type: dna2 equal to string1 plus string2. Nion Enter.
05:53	Sngewbha kynmaw ba: ngim lah ban thep ki sequence da ki alphabets ki bym iahap.
05:59	Kata ka mut, ngim lah ban pynlong kawei ia ka DNA sequence bad ka protein sequence ban ioh ia ka sequence ba thymmai.
06:07	Ki ar tylli ki sequences ki dei ban don kajuh ka alphabet attribute.
06:12	Ban peit ia ka output, type: "dna2". Nion Enter.
06:17	Ka output ka pyni ia ka sequence ba thymmai kaba dei ka jing pyndait lang jong u string1 bad string2.
06:23	Ban ioh ia ka jingjrong jong ka sequence ba thymmai, ngin pyndonkam da ka len function.
06:29	Type: "len" hapoh parenthesis"dna2". Nion Enter.
06:34	Ka output ka pyni ia ka sequence kaba jrong 15 bases.
06:39	Ngi lah ruh ban niew ia ka jingdon jong ki bases bapher bapher ha ka sequence.
06:44	Ban leh ia kane, ngin pyndonkam da ka count function.
06:47	Kum ka nuksa – ban niew ia ka jingdon jong u alanines ba don ha ka sequence, ngi type ia ka command ka ba harum: dna2 dot count hapoh parenthesis hapoh double quotes alphabet A.
07:02	Nion Enter.
07:04	Ka output ka pyni ia ka jingdon jong u alanines ha ka sequence dna2.
07:10	Ban shem ia u jait base ne u dkhot jong u string, ngin pyndonkam da u find function.
07:16	Type: dna2 dot find hapoh parenthesis hapoh double quotes "GC". Nion Enter.
07:26	Ka output ka pyni ia ka jaka ba nyngkong kaba mih ka GC ha u string.
07:32	Ha ka jingshisha ia ka sequence object um ju lah ban pynkylla.
07:35	Ban pynkylla ia ka sequence, ngi dei ban pynkylla iaka sha ka mutable sequence object.
07:41	Ban leh ia kane, type: dna3 equal to dna2 dot to mutable plie bad khang ia u parenthesis. Nion Enter.
07:52	Na ka bynta ka output, type: dna3 Nion Enter.
07:55	Mynta ngin sa lah ban pynkylla ia ka sequence object.
07:59	To ngin ia bujli ia u base na ka sequence.
08:01	Kum ka nuksa – ban bujli ia u base uba don ha ka jaka ba 5 sha ka alanine, type: dna3 within brackets 5 equal to within double quotes alphabet A. Nion Enter.
08:19	Na ka bynta ka output, type: dna3. Nion Enter.
08:24	Khmih thuh ia ka output. Ka cytosine ha ka jaka ba 5 la shah bujli da u alanine.
08:31	Ban bujli ia ka shi bynta jong u string, type ia ka command ba harum.
08:35	Dna3 within brackets 6 semi-colon 10 equal to within double quotes ATGC. Nion Enter.
08:45	Na ka bynta ka output, type: dna3. Nion Enter.
08:52	Ka output ka pyni ia ki 4 tylli ki bases na ka jaka ba 6 haduh 9 kiba shah bujli da ki bases ATGC ba thymmai.
09:01	Shisien ba phi lahdep dep pynkylla ia ka sequence object, pynkylla biang ia ka sha ka “read only” form.
09:07	Type kumne harumdna4 equal to dna3 dot to seq open and close parenthesis. Nion Enter.
09:19	Na ka bynta ka output, type: dna4. Nion Enter.
09:25	To ngin batai lyngkot
09:27	Ha kane ka tutorial, ngi lah pule ban * Generate a random DNA sequence
09:32	Slice ia ka DNA sequence ha ki jaka ba lah buh.
09:36	Pyndait lang ar tylli ki sequence ban ioh ia ka sequence ba thymmai, kata ka mut, ban pynlong kawei.
09:43	Ngi lah pule ruh kumno ban: * use len, count bad find functions.
09:49	Ban pynkylla ia ka sequence object sha ka mutable sequence object bad ban bujli ia u base lane shi bynta jong u string.
09:57	Na ka bynta ka assignment, wad ia uno uno u DNA sequence uba 30 bases.
10:02	Da kaba pyndonkam da u Biopython tools, khein ia ka GC percentage bad molecular weight jong ka sequence.
10:09	Ka assignment ba lah dep jong phi kan long kumne harum.
10:13	Ka output ka pyni ia ka GCcontent ha ka percentage.
10:18	Ka output ka pyni ia ka molecular weight jong ka DNA sequence.
10:23	Kane ka video ka batai lyngkot ia ka Spoken Tutorial project.
10:26	Lada phim don ka bandwidth ba biang, phi lah ban download bad peit ia ka.
10:30	Ngi pynlong workshops bad ai certificates.
10:32	Sngewbha thoh sha ngi
10:35	Ia ka Spoken-Tutorial project la kyrshan da ka National Mission on Education through ICT, MHRD, Government of India.
10:43	ïa kane ka script la pynkylla sha ka Ktien Khasi da u Yuwanki Yolande Kharlukhi na Shillong, Bad ma nga u Hezekiah Lyngdoh, ngan pynkut noh. Khublei ba phi la ïasnoh lang.

Contributors and Content Editors

Hezekiah2016, PoojaMoolya