Biopython/C2/Introduction-to-Biopython/Khasi

Time	Narration
00:01 Ngi pdiang sngewbha ia phi sha kane ka tutorial halor ka Introduction to Biopython.
00:05 Ha kane ka tutorial, ngin ia pule shaphang ki jingling ba kongsan jong ka Biopython
00:10 Ki jingpyntip shaphang ban download bad ban install ha ka Linux Operating System
00:15 Bad ka translation ia ka DNA sequence sha ka protein sequence pyndonkam da ki tiar ka Biopython
00:22 Ban bud ia kane ka tutorial, phi dei ban tip bha ia ka
00:25 Undergraduate Biochemistry lane Bioinformatics
00:29 bad ka basic Python programming
00:31 Peit ia ki Python' tutorial ha ka link bala ai
00:35 Ban record ia kane ka tutorial, nga pyndonkam da ka * Ubuntu OS version 12.04
00:41 Python version 2.7.3
00:44 Ipython version 0.12.1 bad
00:48 Biopython version 1.58.
00:51 Ka Biopython ka dei ka jinglum kyllum ia ki modules na ka bynta ka computational biology
00:57 ka lah ban trei ia kiba suk eh haduh kiba khia tam ki kam kiba donkam ha ka bioinformatics
01:03 Ia ki tiar jong ka Biopython lah ju pyndonkam na ka bynta
01:05 Parsing, kata kaba mut ba wad jingtip na kiba bun ki file formats kum ka FASTA, Genbank, bad kumta ter ter
01:14 Ban download data na ka database websites kum ka NCBI, ExPASY, bad kumta ter ter
01:22 Ban Run ïaki Bioinformatic algorithms kum ka BLAST.
01:26 Ka don ia ki tools ban trei ki kam ha ka rukom ba ryntih
01:31 Kum ka nuksa – ban ioh complements, transcription, translation bad kumta ter ter
01:38 Ki code ban peit ia ka alignment
01:40 bad ki code ban phiah ia ki kam ha ki bynta ba pher ba pher
01:46 Jingpyntip shaphang ban download
01:48 Ka Biopython kam dei kaba wan lang bad ka Python; ngi hap ban download ïaka laka jong
01:54 Ban tip kham bniah, peit ia ka link ba harum.
01:59 Ban install ha ka system Linux
02:02 Install ia ka Python, Ipython bad Biopythonda kaba pyndonkam da ka Synaptic Package Manager
02:08 Ki software ba donkam kin sa install hi
02:13 Ngi hap ban install hi ia ki software jong ka graphic outputs bad plots
02:18 Plie ia ka terminal da kaba nion lang ia u Ctrl, Alt bad T key
02:24 Nga lah dep install lypa ia ka Python, Ipython bad Biopython ha system jong nga
02:30 Plie ia ka Ipython interpreter da kaba type "ipython" bad sa nion Enter
02:35 Ka IPythonprompt ka paw ha screen
02:38 Ban pyntikna ba lah install ia kaBiopython, ha ka prompt, type "import Bio", bad sa press Enter
02:48 Lada phim ioh message error ei ei, ka mut ka Biopython lah install
02:54 Hangne, to ngan pynkynmaw ia phi ba kaPython language ka long case sensitive
02:59 Da phikir bha haba type ki keywords, ki variables lane functions
03:04 Kumba long, u lain haneng, u, “i” ha import dei u lowercase bad u “B”dei u uppercase ha Bio
03:12 Ha kane ka tutorial, ngin pyndonkam da ka Biopythonmodules ban pynkylla ia ka DNA sequence
03:19 Ki rukom pyndonkam ki long harum
03:22 Nyngkong eh, shna ia ka sequence object ban code ia ka DNA strand
03:27 Nangta, sa ka transcription ban code ia ka DNA strand sha ka mRNA
03:32 Khatduh eh, translation jong ka mRNA sha ka protein sequence.
03:37 Ngin pyndonkam da ka coding DNA strand kumba lah pyni ha ka slide, kum ka nuksa
03:42 Ka code naka bynta u protein sequence barit
03:46 ka synjam ba nyngkong ka dei ban shna ia ka sequence object na ka bynta ka coding DNA strand kaba hajrong
03:52 To ngin ia phai biang sha ka terminal
03:55 Ban shna ia ka sequence object, import ïaka ka Seq module na ka Bio package
04:02 Ka Seq module ka ai iaki rukom ban pyllang bad ban shna ia ki sequence objects
04:08 Ha ka prompt, type: from Bio dot Seq import Seq bad sa nion Enter
04:17 Nangta, batai shai ia ki alphabets ha ka strand, haba shna ia ka sequence object jong phi.
04:24 Kata ban thikna lada ia ka sequence jong ki alphabets code na ka bynta ka nucleotides lane amino acids
04:32 Ban leh kumta, ngin pyndonkam da ka IUPAC module na ka Alphabet package
04:38 Ha ka prompt, type: from Bio dot Alphabet import IUPAC. Sa nion Enter
04:48 Kynmaw ba ngi lah pyndonkam import bad from statements ban load "Seq" bad "IUPAC" modules
04:56 Buh ia u sequence object ha ka variable kaba kyrteng cdna
05:01 Ha ka prompt, type: cdna equal to Seq kum ha ki normal string
05:08 Pyndait ia ki sequence hapoh ki double quotes bad parentheses
05:13 Ngi tip ba ka sequence jong ngi ka dei ka DNA ba lyngkhot. Te, type unambiguous DNA alphabet object kum ka argument
05:21 Na ka bynta ka output, type: cdna. Nion Enter
05:26 Ka output ka pyni ia ka DNA sequence kum ka sequence object
05:30 To ngin thoh ia ka coding DNA strand sha ka mRNA. Ba ïadei bad kane.
05:35 Ngin pyndonkam da ka rukom Seq module's built-in “transcribe”
05:39 Type ia ki code kiba harum
05:41 Buh ia ka output ha ka variable mrna
05:45 Ha ka prompt, type: mrna equal to cdna dot transcribe open and close parentheses, nion Enter
05:55 Na ka bynta ka output, type: mrna. Nion Enter
06:01 Khmih thuh ia ka output. Ka rukom transcribe kan bujli ia ka Thiamin ha ka DNA sequence da ka Uracil
06:09 Kaba bud, ban translate ia kane ka mRNA sha ka corresponding protein sequence pyndonkam da ka rukom jong ka translate
06:16 Type ia kine ki code harum: protein equal to mrna dot translate open and close parentheses. Nion Enter
06:27 Ka translate method RNA lane DNA sequence da kaba pyndom ia ka standard genetic code
06:36 Ka output ka pyni ia ka amino acid sequence.
06:40 Ka output ka pyni ruh ia ka thup jingtip naka bynta ka jingdon jong ka stop codons ha ka sequence lah pynkylla.
06:47 Khmih thuh ia u asterisk ha kaba kut jong ka protein sequence. U kdew ia ka stop codon.
06:53 Ha u code ba ha jrong, ngi lah pyndonkam ia u coding DNA strand na ka bynta ka transcription
06:59 Ha Biopython, ka transcribemethod ka treikam tang ha ka coding DNA strand
07:04 Hynrei, ha ka biological system ba shisha, ka rukom iaid jong ka transcription ka sdang da ka template strand
07:11 Lada phi sdang da ka template strand, pynkylla ia ka sha ka coding strand da kaba pyndonkam da ka reverse complement method, kumba lah pyni ha ka terminal.
07:20 Bud ïaki code ba dang sah kumba lah pyni hajrong, na ka bynta ka coding strand
07:24 Da kaba pyndonkam da ki rukom ha Biopython ngi la dep ban pynkylla ia ka DNA sequence sha ka protein sequence.
07:31 Ka DNA sequence kaba ha kano kano ka size lah ban pynkylla sha ka protein sequence da kaba pyndonkam da une u code
07:37 To ngin batai lyngkot. Ha kane ka tutorial, ngi pule shaphang:
07:41 Ki jinglong ba kongsan jong ka Biopython
07:43 Ka thup jingtip shaphang ka download bad installation ha Linux OS
07:48 Ban shna sequence object na ka bynta ka DNA strand
07:52 Transcription ia ka DNA sequence sha ka mRNA
07:56 Translation ia ka mRNA sha ka protein sequence.
08:00 Mynta, na ka bynta ka assignment
08:02 Pynkylla ka DNA sequence ba lah ai sha ka protein sequence.
08:06 Khmih thuh ia ka output
08:08 Ka protein sequence ka don ia ka stop codon
08:11 Kumba ka ju long shisha, pynkylla ia ka DNA shaduh U stop codonuba nyngkong haka frame
08:17 Ka assignment ba lah dep jong phi ka dei ban don ki code kiba harum
08:20 Peit thuh ba ngi lah pyndonkam ka argumentto underscore stop ha ka translate method. Peit ka output
08:27 Ia u stop codon hi khlem shym la pynkylla
08:31 U stop symbol u khlem shah kynthup ha kaba kut ka protein sequence.	08:36 Kane ka video ka batai lyngkot ia ka Spoken Tutorial project
08:39 Lada phim don ka bandwidth ba bha, phi lah ban download bad peit ia ka
08:43 Ka Spoken Tutorial Project team ka pynlong workshop bad ai certificates sha kito ba pass ia ka online test
08:50 Ban tip kham bniah, sngewbha thoh sha ngi
08:53 Ia ka Spoken Tutorial Project la bei tyngka da ka NMEICT, MHRD, Government of India
08:59 Khambuh ki jingtip halor kane ka mission ka don ha kane ka link
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